The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. I. Proliferative response.

Kong, A S; Morse, Stephen I.

doi:10.1084/jem.145.1.151

Cited by 48 publications

(17 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, PTX causes a generalized lymphocytosis that is accompanied by a depletion of lymphocytes in the spleen, lymph node, and thymus due to the failure of cells to migrate back to the peripheral lymphoid tissue (28,29). PTX is also reported to be preferentially mitogenic for T cells (30), and it has been reported to enhance delayed-type hypersensitivity responses in an Ag-specific manner (31,32). The enhanced delayed-type hypersensitivity due to PTX correlates with elevated Ag-specific production of IFN-␥ by these cells (33).…”

Section: Discussionmentioning

confidence: 99%

Genetic Analysis of the Influence of Pertussis Toxin on Experimental Allergic Encephalomyelitis Susceptibility: An Environmental Agent Can Override Genetic Checkpoints

Blankenhorn

Butterfield

Rigby

et al. 2000

The Journal of Immunology

View full text Add to dashboard Cite

Pertussis toxin (PTX) is a potent ancillary adjuvant used to elicit several different autoimmune diseases, including experimental allergic encephalomyelitis (EAE). To delineate the genetics of PTX effect in EAE, we mapped EAE-modifying (eae-m) loci in cohorts of backcross mice immunized with and without PTX. In this study, we analyzed the genetic basis of EAE susceptibility and severity and the intermediate phenotypes of mononuclear cell infiltration, suppuration, and demyelination. In animals immunized with PTX, one major locus, eae9, controls disease susceptibility and severity. Eae9 also regulates the extent of mononuclear cell infiltration of the spinal cord in male mice. Without PTX, five eae-m loci were noted, including three new loci in intervals on chromosomes 8 (eae14), 10 (eae17), and 18 (eae18). Taken together, these results suggest that eae9 controls the effects of PTX in EAE susceptibility, and is capable of overriding the other genetic checkpoints in the pathogenesis of this disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic Analysis of the Influence of Pertussis Toxin on Experimental Allergic Encephalomyelitis Susceptibility: An Environmental Agent Can Override Genetic Checkpoints

Blankenhorn

Butterfield

Rigby

et al. 2000

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…Long-standing clinical observations of marked lymphocytosis during the paroxysmal phase ofwhooping cough (31) and several laboratory studies (32)(33)(34) Analysis ofphosphoinositides. Inositol-containing lipids were analyzed by the methods of Berridge (9).…”

Section: Introductionmentioning

confidence: 99%

Bacterial toxins affect early events of T lymphocyte activation.

Stewart¹,

Prpić²,

Johns³

et al. 1989

J. Clin. Invest.

View full text Add to dashboard Cite

The effects of pertussis toxin and cholera toxin on early events of T lymphocyte activation were examined in the T lymphocyte cell line, Jurkat. Pertussis toxin treatment of these T cells increased inositol phosphates production and led to increases in intracellular free calcium concentration. These effects were produced by the isolated B (binding) subunit of pertussis toxin, alone. Inositol phosphates production resulting from perturbation of the T cell antigen receptor-CD3 complex by MAb was not affected by pertussis toxin treatment but was markedly inhibited by cholera toxin. This effect of cholera toxin paralleled elevations in cAMP content. However, forskolin, in concentrations equipotent for cAMP production, was a weaker inhibitor of inositol phosphates production. Cholera toxin inhibition of inositol phosphates production did not result from inhibition of baseline incorporation of inositol into phosphoinositide substrates of phospholipase C. These studies underline the complexity of toxin effects on cellular systems and suggest that other approaches will be required to implicate guanine nucleotide-binding regulatory proteins in control of the early events of T lymphocyte activation. However, the data presented here provide a molecular basis for the clinical observations of lymphocytosis and the in vitro observations of lymphocyte mitogenesis after pertussis toxin stimulation.

show abstract

“…Recently, the lymphocytosis promoting factor (LPF)' has been purified from B. pertussis culture supernatant fluids (3). In addition to its in vivo effects, LPF was found by Kong and Mlorse (4,5) to be a potent mitogen for murine lymphocytes in vitro. LPF stimulated cells from lymph nodes and spleen but not from the thymus or bone marrow.…”

Section: Introductionmentioning

confidence: 99%

The Mitogenic Effect of the Lymphocytosis Promoting Factor from Bordetella Pertussis on Human Lymphocytes

Morse¹,

Kong²,

Lindenbaum³

et al. 1977

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T The purified lymphocytosis promoting factor (LPF) from Bordetella pertussis was found to be a potent mitogen for peripheral blood lymphocytes (PBL) PBL from most patients with chronic lymphatic leukemia and lymphosarcoma cell leukemia were even less responsive to LPF than to phytohemagglutinin, whereas PBL from patients with lymphosarcoma usually responded to both mitogens. It can be inferred from the results of experiments with both normal and leukemic cells that LPF, which is a murine thymtusderived (T)-cell mitogen, is also a T-cell mitogen for human PBL. The exact cell requirement and mode of action, however, are as yet unknown.

show abstract

The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. I. Proliferative response.

Cited by 48 publications

References 11 publications

Genetic Analysis of the Influence of Pertussis Toxin on Experimental Allergic Encephalomyelitis Susceptibility: An Environmental Agent Can Override Genetic Checkpoints

Genetic Analysis of the Influence of Pertussis Toxin on Experimental Allergic Encephalomyelitis Susceptibility: An Environmental Agent Can Override Genetic Checkpoints

Bacterial toxins affect early events of T lymphocyte activation.

The Mitogenic Effect of the Lymphocytosis Promoting Factor from Bordetella Pertussis on Human Lymphocytes

Contact Info

Product

Resources

About