The in vivo and in vitro oxidation of molecular form 1 of biliverdin reductase to molecular form 3 by diamide

Abstract

The in vivo and in vitro oxidation of molecular form 1 of biliverdin reductase to molecular form 3 by diamide Administration of phenylhydrazine to rats converted molecular form f of the liver biliverdia reductase into its disulgde bridN dimer (molecular farm 3). This oxidative dimerization was shown not to be mediated by the NAD"-dependent dehydrogenase [(1984) Rio&em. Riophys. Res. Commun. I2 1,2492541. Administration of diamide produced the same conversion, Although hepatic levels of GSH alsa decreased, no mixed disulfides of the reductase and GSH could be detected. Administration of the antioxidants allopurinol and a-tocopherol together with the diamide did not at%ct this conversion of molecular forms produced by the latter. The diamide also axidized molecular form 1 of biliverdin mductase in vitro and molecular form 3 was formed. The ohemical oxidation took place at a high rate and was partially inhibited by GSH but not by cysteine.

Removal of a dimeric form of surfactant protein C from mouse lungs: its acceleration by reduction

“…BSO and DA, potent inhibitors of GSH, are widely used to inhibit GSH action (11,23). In vivo, we could lower the GSH level in lavage fluid to about one-fourth of the control value with BSO and with a high dose of DA.…”

Inhibitory Effects of Palm Tocotrienol-Rich Fraction Supplementation on Bilirubin-Metabolizing Enzymes in Hyperbilirubinemic Adult Rats

“…Therefore, it can be postulated that increased activity of the enzyme might reflect the increased formation of bilirubin, the product of interest in this study. Phenylhydrazine was demonstrated to increase the activity of the hepatic biliverdin reductase with a reduction in glutathione content [42], indicating an increase in oxidative stress. However, α-tocopherol had no significant effect on the enzyme activity [42].…”

“…Phenylhydrazine was demonstrated to increase the activity of the hepatic biliverdin reductase with a reduction in glutathione content [42], indicating an increase in oxidative stress. However, α-tocopherol had no significant effect on the enzyme activity [42]. Studies regarding the effect tocotrienol on biliverdin reductase activity or expression is still lacking.…”