The in vivo antitumor activity of LHRH targeted methotrexate–human serum albumin nanoparticles in 4T1 tumor-bearing Balb/c mice

Taheri, Azade; Dinarvand, Rassoul; Ahadi, Fatemeh; Khorramizadeh, Mohammad Reza; Atyabi, Fatemeh

doi:10.1016/j.ijpharm.2012.04.033

Cited by 54 publications

(37 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Albumin--paclitaxel conjugate was shown to accumulate in the tumor by EPR effect [40][41][42]. Several chemotherapeutic drug conjugates of serum albumin have shown promising antitumor activity in preclinical models, and albumin--paclitaxel conjugate has successfully reached the market [43][44][45][46][47][48][49]. Yao et al (2013) prepared SN38 covalently conjugated to the only free sulfhydryl positioned at cysteine-34 of bovine serum albumin (BSA) site through a thiol-binding spacer.…”

Section: Albumin--sn38 Conjugatementioning

confidence: 99%

Challenges in SN38 drug delivery: current success and future directions

Palakurthi

2015

Expert Opinion on Drug Delivery

View full text Add to dashboard Cite

The challenges in SN38 drug delivery may be overcome by two ways: ensuring multiple layers of protection against degradation and slow but sustained release of therapeutically effective drug concentrations. It may also be achieved by preparing a polymer-drug conjugate and further encapsulating the conjugate in suitable carrier system; tumor-targeted SN38 delivery by using immunoconjugates, enzyme-activated prodrug therapy and antibody-directed nanoparticle delivery. However, selection of a suitable ligand for tumor targeting and use of safe and biocompatible nanoparticle systems play an important role in realizing this goal.

show abstract

Section: Albumin--sn38 Conjugatementioning

confidence: 99%

Challenges in SN38 drug delivery: current success and future directions

Palakurthi

2015

Expert Opinion on Drug Delivery

View full text Add to dashboard Cite

show abstract

“…Distribution studies of fluorescent LNEs in tumor-induced mice by using the IVIS Female Balb/c mice (6-8 weeks) were subcutaneously xenografted by MDA-MB 231 cells and tumors were developed in three weeks (Taitz et al, 1995;Taheri et al, 2012;Devika et al, 2013). After tumor growth, mice (n ¼ 3 for each group) were anaesthetized with 2% isoflurane and were injected into the tail vein with fluorescent tagged LNEs at a dose of 10 mg/kg (Table 1).…”

Section: Estimation Of Albumin Bound To Globulesmentioning

confidence: 99%

Albumin anchored docetaxel lipid nanoemulsion for improved targeting efficiency – preparation, characterization, cytotoxic, antitumor and in vivo imaging studies

et al. 2015

View full text Add to dashboard Cite

The aim was to develop albumin anchored docetaxel lipid nanoemulsion (ALNE) for improving tumor targeted delivery. The O/W lipid nanoemulsion, LNEs were prepared by homogenization and ultrasonication processes. The size of globules and zeta potential were measured by Malvern Zetasizer. Albumin was coupled to stearylamine containing lipid nanoemulsion (SALNE) globules using water soluble EDC reaction. The drug content and entrapment efficiencies for the LNEs were determined by the high-performance liquid chromatography. The in vitro cytotoxic studies of the delivery systems were performed on MCF-7 and Hela cells. The IC 50 values of ALNE on both the cell lines were statistically significant. The in vivo antitumor activity was tested on solid tumors induced in C57BL/6 mice. This study revealed that the percentage tumor inhibition for the groups treated with DLNE, SALNE and ALNE when compared with untreated control was found to be 55.62 ± 5.41%, 54.27 ± 4.85% and 80.01 ± 2.74%, respectively. Furthermore, in vivo distribution studies were carried out in breast cancer MDA-MB231 xenografted Balb/c mice. The LNEs were loaded with fluorescent DiD oil and the distribution in different organs after 6 h was tracked using Caliper life sciences in vivo imaging system. The studies revealed that ALNE was superior in tumor targeting activity when compared with DLNE and SALNE by 3.04 and 2.26 folds, respectively. The average radiance values of ALNE on the tumor tissue were statistically significant when compared with DLNE, SALNE at p50.01. In addition, this strategy can become a platform technology for other lipophilic drugs to target tumors. KeywordsAlbumin tagged docetaxel lipid emulsion, antitumor study, fluorescent imaging study, improved tumor delivery, tumor targeting History

show abstract

“…[28][29][30] In particular, albumin nanoparticles could preferentially accumulate in tumor sites due to their remarkable targetability to tumors. 31 They permeate leaky blood vessels via an efficient extravasation process. 32 Thus, whether the coating of plasma membrane can improve the uptake efficiency and targeting therapy of albumin nanoparticles remains to be explored.…”

Section: Introductionmentioning

confidence: 99%

<p>Paclitaxel-Loaded Macrophage Membrane Camouflaged Albumin Nanoparticles for Targeted Cancer Therapy</p>

Cao

Tan

Zhu

et al. 2020

IJN

View full text Add to dashboard Cite

Background: Melanoma is the most common symptom of aggressive skin cancer, and it has become a serious health concern worldwide in recent years. The metastasis rate of malignant melanoma remains high, and it is highly difficult to cure with the currently available treatment options. Effective yet safe therapeutic options are still lacking. Alternative treatment options are in great demand to improve the therapeutic outcome against advanced melanoma. This study aimed to develop albumin nanoparticles (ANPs) coated with macrophage plasma membranes (RANPs) loaded with paclitaxel (PTX) to achieve targeted therapy against malignant melanoma. Methods: Membrane derivations were achieved by using a combination of hypotonic lysis, mechanical membrane fragmentation, and differential centrifugation to empty the harvested cells of their intracellular contents. The collected membrane was then physically extruded through a 400 nm porous polycarbonate membrane to form macrophage cell membrane vesicles. Albumin nanoparticles were prepared through a well-studied nanoprecipitation process. At last, the two components were then coextruded through a 200 nm porous polycarbonate membrane. Results: Using paclitaxel as the model drug, PTX-loaded RANPs displayed significantly enhanced cytotoxicity and apoptosis rates compared to albumin nanoparticles without membrane coating in the murine melanoma cell line B16F10. RANPs also exhibited significantly higher internalization efficiency in B16F10 cells than albumin nanoparticles without a membrane coating. Next, a B16F10 tumor xenograft mouse model was established to explore the biodistribution profiles of RANPs, which showed prolonged blood circulation and selective accumulation at the tumor site. PTX-loaded RANPs also demonstrated greatly improved antitumor efficacy in B16F10 tumor-bearing mouse xenografts. Conclusion: Albumin-based nanoscale delivery systems coated with macrophage plasma membranes offer a highly promising approach to achieve tumor-targeted therapy following systemic administration.

show abstract

The in vivo antitumor activity of LHRH targeted methotrexate–human serum albumin nanoparticles in 4T1 tumor-bearing Balb/c mice

Cited by 54 publications

References 27 publications

Challenges in SN38 drug delivery: current success and future directions

Challenges in SN38 drug delivery: current success and future directions

Albumin anchored docetaxel lipid nanoemulsion for improved targeting efficiency – preparation, characterization, cytotoxic, antitumor and in vivo imaging studies

<p>Paclitaxel-Loaded Macrophage Membrane Camouflaged Albumin Nanoparticles for Targeted Cancer Therapy</p>

Contact Info

Product

Resources

About