The in vivo estrogenic modulatory effect of bisphenol A (BPA) on Oreochromis mossambicus and prevention of early maturation of ovary by conjugates of intracellular laccase and silica nanoparticles

Abstract

Immobilized laccase can significantly reduce thein vivoestrogenic effect of BPA and protect organisms from endocrine disrupting effect and extinction.

Efficient Removal of Cadmium Using Edible Fungus and Its Quantitative Fluorimetric Estimation Using (Z)-2-(4H-1,2,4-Triazol-4-yl)iminomethylphenol

“…After 24 h, both cell lines were treated with various concentrations of the synthesized probe (50–2000 μg/mL) 37,38 and incubated for 48 h. Resazurin (1 μM) was added in all probe treated AGS and MCF-12F cells, and after 4 h of incubation, the resorufin formation was measured fluorimetrically at 540 nm excitation and 590 nm emission.…”

“…To check the cytotoxic effect of the synthesized probe, AGS and MCF-12F cell lines were used, and resazurin assay was performed in 96 well plates (5000 cells/well) using DMEM (F12/HAM) culture media. After 24 h, both cell lines were treated with various concentrations of the synthesized probe (50–2000 μg/mL) , and incubated for 48 h. Resazurin (1 μM) was added in all probe treated AGS and MCF-12F cells, and after 4 h of incubation, the resorufin formation was measured fluorimetrically at 540 nm excitation and 590 nm emission.…”

Early maturation and liver necrosis in the fingerling stage of Oreochromis mossambicus due to BPA can cause an ecological imbalance

“…First strand cDNA was constructed using RevertAid H Minus First Strand cDNA synthesis kit (Thermo SCIENTIFIC-K1621) for all fingerlings and the differential vitellogenin gene expression was quantified using an ABI Prism 7000 qRT- PCR machine. 2 Each 10 μL qRT-PCR reaction contained 5 μL of 2× SYBr Green Mix, 1 μL of cDNA, 2 μL of MilliQ, 1 μL of (10 pmol μL −1 ) forward and reverse vitellogenin gene (Vtg) specific primers (F-TCGAGCTGGGGTTAAAATC, R-TGGCAGTGGTTCAGGTC). 22 The thermal cycle program was set to 94 °C (3 minutes), followed by 35 cycles of 94 °C (30 seconds), 55 °C for (30 seconds), and 72 °C (45 seconds).…”

“…The peak appearing in the NaCl gradient line was collected separately and conrmed using a vitellogenin specic ELISA kit [CUSABIO Grouper Vitellogenin (VTG) ELISA Kit-CSB-EI4116Fh]. 2,32 A plasma sample was used in a similar manner for the detection of vitellogenin secretion. Aromatase is secreted from the brain in the presence of xenobiotic contaminants.…”

“… 1 Endocrine disrupting chemicals can alter the endocrine system by affecting these hormones. Some EDCs can resemble key hormones 2 and as a consequence, the hormonal regulation can be altered. 3 Exposure to EDCs has been associated with numerous adverse reproductive defects, 4,5 such as reduction in female fecundity as well as male sperm motility, longer time to conception, and massive miscarriage rates.…”