The in vivo fertility of cauda epididymal spermatozoa in the horse

Morris, L. H. A.; Tiplady, C.; Allen, W. R.

doi:10.1016/s0093-691x(02)00742-2

Cited by 53 publications

(7 citation statements)

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“…Previous studies have shown that despite certain differences in the characteristics of epididymal spermatozoa and freshly ejaculated sperm, the former can be effectively used for in vitro fertilization (IVF) and artificial insemination (AI) of farm animals, including pigs [ 4 ], bulls [ 5 ] and stallions [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

The Quality and Fertilizing Potential of Red Deer (Cervus elaphus L.) Epididymal Spermatozoa Stored in a Liquid State

Dziekońska

Koziorowska-Gilun

Kordan

et al. 2022

IJMS

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The aim of this study was to assess the quality and fertilizing potential of red deer epididymal spermatozoa stored in a liquid state for up to 11 days (D11). In Experiment 1, sperm quality was determined. In Experiment 2, the efficiency of in vitro fertilization (IVF) and artificial insemination (AI) of stored sperm were evaluated. An analysis of sperm quality on D5 of storage revealed a decrease (p < 0.05) in motility and morphology, and a higher proportion of apoptotic spermatozoa. On D1, D7 and D10, the total motility of sperm for IVF and AI was determined to be 82.6%, 71.0% and 64.8%, respectively. The results of IVF and AI demonstrated that the fertilizing potential of spermatozoa differs between days of storage. The percentage of blastocysts was higher when oocytes were fertilized on D1 (17.4 %) compared to D7 (8.5%) and D10 sperm (10.5%). Differences were noted in the pregnancy rates of inseminated hinds. The insemination with D1, D7 and D10 sperm led to live births (33% from D7 and D10). The results indicate that the quality of red deer epididymal spermatozoa remains satisfactory during ten days of storage in a liquid state, and that these spermatozoa maintain their fertility potential.

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Section: Introductionmentioning

confidence: 99%

The Quality and Fertilizing Potential of Red Deer (Cervus elaphus L.) Epididymal Spermatozoa Stored in a Liquid State

Dziekońska

Koziorowska-Gilun

Kordan

et al. 2022

IJMS

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“…It is necessary to consider that the physiology of each segment of the epididymis may affect the extent to which sperm DNA becomes fragmented [14] [47], with associated influence on the quality of the embryo and its implantation [8] [48]. For instance, pregnancy rates in horses with the use of epididymal sperm have ranged from 8% -92% [49] and this variation could be associated with sperm DNA fragmentation.…”

Section: Discussionmentioning

confidence: 99%

Azteca Breed Horse Epididymal Sperm Evaluation: A Comparison of Head, Corpus and Cauda Sperm Quality

Parra-Forero¹,

Mendoza

Góngora³

et al. 2015

ARSci

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The main objective of this study was to determine differences in sperm recovered from the three segments of the epididymis, i.e., head, corpus and cauda, using routine measurements of sperm quality and DNA fragmentation assessment. There was a larger sperm concentration (P < 0.05) in the head (3.34 × 10 6 sperm/mL) compared with the corpus and cauda. The percentage of normal morphology was higher in the cauda but had lower motility (P < 0.05). The DNA fragmentation index was higher for sperm obtained from the head and body of the epididymis (P < 0.01) compared with the cauda. The DNA fragmentation index values were 12% higher (P < 0.01) using Wright stain compared with DAPI stain in sperm from the head and corpus of the epididymis, but similar in the cauda. Spermatobioscopy parameters match the maturation changes that occur in the epididymal tract with higher quality sperm being from the cauda, but with a lower concentration compared to sperm found in the head.

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“…for sperm harvesting and processing 24-48 h after death or castration (Stawicki et al, 2016), and others have examined the addition of seminal plasma to epididymal sperm (Moore et al, 2005;Morris et al, 2002).…”

Section: Epididymal S Perm Cryop Re S Erv Ationmentioning

confidence: 99%

“…A number of publications offer instructions to collect and process epididymal sperm (Bruemmer, 2006; Monteiro et al., 2011; Papa et al., 2008). Guidelines have been developed for shipping epididymides at 5°C for sperm harvesting and processing 24–48 h after death or castration (Stawicki et al., 2016), and others have examined the addition of seminal plasma to epididymal sperm (Moore et al., 2005; Morris et al., 2002).…”

Section: Epididymal Sperm Cryopreservationmentioning

confidence: 99%

Stallion semen cryopreservation: Frozen semen preparation, handling and breeding

Pasch

2024

Equine Veterinary Education

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SummaryThe use of cryopreserved stallion semen is a significant aspect of equine reproduction, despite the tendency for lower per cycle pregnancy rates than cooled or fresh semen. This decreased fertility of thawed frozen semen is due in part to the innate subfertility of the chosen male breeding population, but also to the direct insults to the sperm cell during the freezing and subsequent thawing process. The mare aspect of frozen semen breeding is intensively managed to compensate for the aforementioned damages, and acceptable pregnancy rates can be achieved. Here we describe the stallion semen cryopreservation process including semen collection, expected causes of damage in processing, freezing and thawing, mare management and future directions.

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The in vivo fertility of cauda epididymal spermatozoa in the horse

Cited by 53 publications

References 0 publications

The Quality and Fertilizing Potential of Red Deer (Cervus elaphus L.) Epididymal Spermatozoa Stored in a Liquid State

The Quality and Fertilizing Potential of Red Deer (Cervus elaphus L.) Epididymal Spermatozoa Stored in a Liquid State

Azteca Breed Horse Epididymal Sperm Evaluation: A Comparison of Head, Corpus and Cauda Sperm Quality

Stallion semen cryopreservation: Frozen semen preparation, handling and breeding

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