The inactivation of Bacillus subtilis spores at low concentrations of hydrogen peroxide vapour

Malik, Danish J.; Shaw, C.M.; Rielly, Chris D.; Shama, Gilbert

doi:10.1016/j.jfoodeng.2012.08.031

Cited by 24 publications

(14 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Comparison of the D values obtained for spores of B. subtilis obtained by Malik et al (2013) using similar experimental conditions to those for C. difficile obtained in the current work is shown in Table II. In each case the spores were exposed in the form of monolayers deposited and Geobacillus stearothermophilus spores deposited on a variety of indoor surface materials and exposed to HPV.…”

Section: Discussionmentioning

confidence: 53%

An Investigation into the Inactivation Kinetics of Hydrogen Peroxide Vapor Against Clostridium difficile Endospores

Malik

Shaw

Shama

et al. 2016

Chemical Engineering Communications

Self Cite

View full text Add to dashboard Cite

C. difficile spores are resistant to routine cleaning agents and are able to survive on inanimate surfaces for long periods of time. There is increasing evidence of the importance of the clinical environment as a reservoir for pathogenic agents and as a potential source of healthcare-associated infections (HCAIs). In this context, to reduce the risk of cross-transmission, terminal disinfection of hospital wards and isolation rooms using hydrogen peroxide vapor (HPV) is attracting attention. Spores of C. difficile (ribotype 027) were exposed to constant concentrations of HPV ranging between 11 and 92 mg m À3 (ppm) for a range of exposure times in a specially designed chamber. The inactivation data thus obtained was fitted using the modified Chick-Watson inactivation model to obtain decimal reduction values (D values). D values ranged from 23 to 1.3 min at HPV concentrations of 11 and 92 ppm, respectively. We present a simple mathematical model based on the inactivation kinetic data obtained here to estimate the efficacy of commercial HPV processes used in healthcare environmental decontamination. C. difficile spores showed linear inactivation kinetics at steady HPV concentrations ranging between 10 and 90 ppm. The data obtained here was used to provide estimates of the inactivation efficacy of commercial HPV process cycles, which employ unsteady HPV concentrations during the decontamination process.

show abstract

Section: Discussionmentioning

confidence: 53%

An Investigation into the Inactivation Kinetics of Hydrogen Peroxide Vapor Against Clostridium difficile Endospores

Malik

Shaw

Shama

et al. 2016

Chemical Engineering Communications

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, long-lived water-related species such as hydrogen peroxide produced from the reactions contributed to the inactivation of spores. H 2 O 2 is one of the most well-known sporicidal agents and widely used in the sterilization processes [48][49][50]. In addition, reactive nitrogen species, e.g.…”

Section: Discussionmentioning

confidence: 99%

Sporicidal properties from surface micro-discharge plasma under different plasma conditions at different humidities

et al. 2014

View full text Add to dashboard Cite

In the current study, bacterial endospores of Geobacillus stearothermophilus are exposed to the surface micro-discharge plasma for 5 min and the humidity and power consumption are varied. At the low humidity of 5.5 ± 0.5 g m −3 , almost no sporicidal effect (<0.5 log) is observed. At the high humidity of 17.9 ± 0.6 g m −3 , the spore reduction increases monotonically up to 3.5 log with increasing power consumption. At a humidity of 10.4 ± 0.6 g m −3 , the spores are inactivated in a limited range of power consumption with a maximum reduction of ∼2.5 log. The survival curves show a single-slope decrease of the spores. The contribution of heat and UV to the sporicidal effect as well as the inactivation of spores by the short-lived species from the plasma are ruled out. The concentration of ozone, one indicator for the long-lived species, is measured and no correlation with the sporicidal effect is found. In conclusion, water-related reactive species, e.g. hydrogen peroxide, appear to be responsible for the sporicidal effect under the investigated conditions. Furthermore, condensation of water at high humidity enables the plasma-activated water containing both long-lived and short-lived reactive species to contribute to the sporicidal effect.

show abstract

“…No entanto, sabe-se que o uso desses produtos pode interferir positivamente a microbiota nativa do solo, mesmo sendo a aplicação foliar o modo de dispensa. Este fato já foi constatado por Malik et al (2012), que indica que o uso de biofertilizantes pode aumentar a disponibilidade de nutrientes às plantas pelo processo de mineralização através da matéria orgânica do solo e atuar, indiretamente, através do retorno dos nutrientes imobilizados pela massa microbiana. Já Prates e Medeiros (2001) dizem que a maior importância de um biofertilizante como fertilizante, não está nos quantitativos dos seus nutrientes, mas na diversidade da composição mineral, que pode formar compostos quelatizados e esses disponibilizados pela atividade biológica como ativador…”

Section: Conclusãounclassified

Untitled

2019

Ens Cien C Biol Agra Saúde

View full text Add to dashboard Cite

Este estudo avaliou a influência da aplicação foliar de diferentes concentrações do Fert Bokashi® no desenvolvimento de plantas de quatro cultivares de soja. O experimento foi realizado em casa de vegetação durante 75 dias, utilizando-se vasos de cinco litros distribuídos em quatro blocos, para testar diferentes concentrações do biofertilizante (10% ativado; 5; 2,5; 1% e testemunha). Foram realizadas duas aplicações foliares do produto, nos estágios V3 e V6. As variáveis analisadas foram comprimento, massa fresca e seca da parte aérea e radicular da planta; número de folhas; número e massa de nódulos; número e massa fresca e seca de vagens. O efeito da aplicação do biofertilizante variou entre as diferentes cultivares avaliadas, sendo que a cultivar BR5 284 não foi afetada pelo uso do biofertilizante. As demais cultivares (SYN 9070 RR, AS 3730 IPRO e M6410 IPRO) foram afetadas, negativamente, em uma ou mais características de crescimento e/ou de produção, quando submetidas à aplicação a 10%. A aplicação na dose de 1% favoreceu somente a cultivar M6410 IPRO em termos de crescimento radicular e massa fresca de nódulos.

show abstract

The inactivation of Bacillus subtilis spores at low concentrations of hydrogen peroxide vapour

Cited by 24 publications

References 28 publications

An Investigation into the Inactivation Kinetics of Hydrogen Peroxide Vapor Against Clostridium difficile Endospores

An Investigation into the Inactivation Kinetics of Hydrogen Peroxide Vapor Against Clostridium difficile Endospores

Sporicidal properties from surface micro-discharge plasma under different plasma conditions at different humidities

Untitled

Contact Info

Product

Resources

About