The InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of cuckoo wasps, with the description of new species from the Iberian Peninsula (Hymenoptera, Chrysididae)

Abstract

DNA barcoding technologies have provided a powerful tool for the fields of ecology and systematics. Here, we present a part of the InBIO Barcoding Initiative Database: contribution to the knowledge on DNA barcodes of cuckoo wasps (Hymenoptera, Chrysididae) dataset representing 144 specimens and 103 species, covering approximately 44% of the Iberian and 21% of the European fauna. The InBIO Barcoding Initiative (IBI – DNA Barcoding Portuguese terrestrial invertebrate biodiversity) aims to fill the barcoding gap for the terrestrial invertebrate taxa. All DNA extractions are deposited in the IBI collection at CIBIO, Research Center in Biodiversity and Genetic Resources and specimens are deposited in the University of Mons collection (Belgium) and in the Natur-Museum in Lucerne (Switzerland). This dataset increases the knowledge on the DNA barcodes and distribution of 102 species of cuckoo wasps. A total of 52 species, from 11 different genera, were new additions to the Barcode of Life Data System (BOLD), with DNA barcodes for another 44 species added from under-represented taxa in BOLD. All specimens have their DNA barcodes publicly accessible through the BOLD online database. Nine cuckoo wasp species are newly recorded for Portugal. Additionally, two new species for science are described: Chrysis crossi Rosa, sp. nov. from southern Portugal and Hedychridium calcarium Rosa, sp. nov. from eastern Spain. Several taxonomic changes are proposed and Hedychrum rutilans Dahlbom, 1845 is found to consist of two different taxa that can be found in sympatry, Hedychrum rutilans s. str. and Hedychrum viridaureum Tournier, 1877 stat. nov. Stilbum westermanni Dahlbom, 1845 stat. nov. is confirmed as distinct from Stilbum calens (Fabricius, 1781), with the latter species not confirmed as present in Iberia; barcoded Stilbum material from Australia is distinct and represents Stilbum amethystium (Fabricius, 1775) sp. resurr.; Portuguese material identified as Hedychridium chloropygum Buysson, 1888 actually belongs to Hedychridium caputaureum Trautmann & Trautmann, 1919, the first confirmed record of this species from Iberia. Philoctetes parvulus (Dahlbom, 1845) is confirmed to be a synonym of Philoctetes punctulatus (Dahlbom, 1845). Chrysis lusitanica Bischoff, 1910 is confirmed as a valid species. Chrysis hebraeica Linsenmaier, 1959 stat. nov. is raised to species status.

The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese moths

“…The InBIO Barcoding Initiative (IBI) makes use of High-Throughput Sequencing technologies to construct a reference collection of morphologically identified Portuguese specimens and respective DNA barcodes (e.g. Rebelo et al 2020 , Ferreira et al 2021 , Oliveira et al 2021 , Pauperio et al 2023 , Rosa et al 2023 ). DNA barcoding offers a rapid, cost-effective alternative tool for both the identification of described species and the discovery of new ones ( Hebert et al 2003 , Savolainen et al 2005 , Mitchell 2008 , our papers).…”