The incidence and significance of H. influenzæ in chronic bronchiectasis

The Agglutination Reactions of Haemophilus Paraphrophilus and H. Paraphrohaemolyticus, and Some Observations on the Agglutination of H. Aphrophilus and H. Haemoglobinophilus (H. Canis)

“…Non-capsulate H. infruenzae strains were found to be heterogeneous (Park, Williams and Cooper, 1918;Valentine and Cooper, 1919;Kristensen, 1922;Allison, Gordon and Zinnemann, 1943), but different polysaccharide capsular antigens were demonstrated by Pittman (1931) and six serological types are recognised on this basis. Khairat (1940) noted that the species H. aphrophilus was serologically distinct from the X-dependent, V-independent species H. haemogZobinophilus and H. hfluenzaemurium; he could not determine whether his new species H. aphrophilus was homogeneous or not, because all his strains had been isolated from one subject.…”

Adult Chronic Bronchitis--the Infective Factor and its Treatment

“…Each specimen was cultured on fresh blood and heated blood (chocolate) agar. A simple inoculation technique previously described (Allison et al, 1943;Allibone et al, 1956) was employed. The cultures were examined after 24 and 48 hours' incubation.…”

Meningitis due to Pittman and Non-Pittman Strains of H. influenzae

“…Neither of these two cases was treated with sulphanilamide or any of its compounds. Pittman (1931) has shown that rough strains, and Allison, Gordon, and Zinnemann (1943) have shown that respiratory strains not belonging to the Pittman types, are virulent for white mice, although this virulence is lower than that of strains of the Pittman types. In view, therefore, of the small number of cases reported as being due to non-capsulated strains of H. influenzae, and their theoretical importance, we are recording the following cases of meningitis, which responded very well to sulphapyridine and which could be shown not to belong to any of the Pittman types.…”

“…Agglutinations were carried out with the technique described by Allison, Gordon, and Zinnemann (1943). Neither strain gave a specific agglutination with the agglutinating H. influenzae sera a to f when incubation was carried out at 37' C. An agglutinating serum, prepared by immunizing a rabbit with the strain isolated from Case 1, did not agglutinate the Pittman strains a to f at 37' C., nor did the serum agglutinate the strain from Case 2, but it gave a good agglutination with its own strain.…”