The incidence of apoptotic bodies in membrana granulosa can predict prognosis of ova from patients participating in in vitro fertilization programs

Nakahara, Kenji; Saito, Hidekazu; Saito, Toki; Ito, Mariko; Ohta, Nobuhiko; Takahashi, Toshifumi; Hiroi, Masahiko

doi:10.1016/s0015-0282(97)81521-x

Cited by 133 publications

(101 citation statements)

References 21 publications

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“…In some studies a significantly lower incidence of apoptosis in follicles with an oocyte compared to empty ones was reported; the fertilization rate was higher in oocytes originating from the follicles with less apoptosis [3,23,24]. However, we did not observe a significant difference in the percentage of apoptotic granulosa cells between the follicles with a fertilized and those with a nonfertilized corresponding oocyte.…”

Section: Discussioncontrasting

confidence: 82%

“…Granulosa cells are essential in normal follicular maturation process since they produce steroidal hormones and growth factors, and they play a crucial role in follicular atresia [2]. Apoptosis of granulosa cells seems to have a negative effect on conception and pregnancy rates in an in vitro fertilization and embryo transfer (IVF-ET) programme [3,4]. A higher incidence of apoptotic granulosa cells has been associated with a higher incidence of empty follicles, fewer oocytes retrieved and poorer quality of oocytes and embryos [3].…”

Section: Introductionmentioning

confidence: 99%

“…Apoptosis of granulosa cells seems to have a negative effect on conception and pregnancy rates in an in vitro fertilization and embryo transfer (IVF-ET) programme [3,4]. A higher incidence of apoptotic granulosa cells has been associated with a higher incidence of empty follicles, fewer oocytes retrieved and poorer quality of oocytes and embryos [3]. Furthermore, different ovarian stimulation protocols affect the incidence of apoptotic granulosa cells differently [5].…”

Section: Introductionmentioning

confidence: 99%

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Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Jančar

Kopitar

Ihan

et al. 2007

J Assist Reprod Genet

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Purpose: The aim was to establish the impact of human granulosa cell apoptosis and reactive oxygen species (ROS) production on fertilization competence of the oocyte, embryo developmental stage and implantation rate.Methods: Thirty women undergoing IVF-ET for tubal factor infertility were included; GnRH antagonists and gonadotrophins were used for ovarian stimulation. Granulosa cells were isolated from each aspirated follicle using gradient centrifugation. Apoptosis was studied by flow cytometry using annexin V and propidium iodide. ROS production was studied with hydroethidine staining and analyzed by flow cytometry.Results: There were no differences in characteristics of granulosa cells between the follicles with fertilized and nonfertilized oocytes. The analyzed characteristics of granulosa cells in corresponding follicles had no effect on embryo developmental stage on day 5. The percentage of ROS producing granulosa cells was lower in the follicles giving rise to blastocysts that resulted in implantation compared to those that did not (39.9% versus 69.9%, P = 0.031).Conclusions: Apoptosis and ROS production in granulosa cells have no significant impact on fertilization and do not correlate with the development of blastocysts. An increased

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Section: Discussioncontrasting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Jančar

Kopitar

Ihan

et al. 2007

J Assist Reprod Genet

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“…Investigators have previously demonstrated that high rates of cumulus cell apoptosis are associated with a poor outcome of assisted reproductive technology (ART) procedures [15,16].…”

Section: Discussionmentioning

confidence: 99%

Apoptotic Analysis of Cumulus Cells for the Selection of Competent Oocytes to Be Fertilized by Intracytoplasmic Sperm Injection (ICSI)

et al. 2015

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Oocyte quality is one of the main factors for the success of in vitro fertilization protocols. Apoptosis is known to affect oocyte quality and may impair subsequent embryonic development and implantation. The aim of this study was to investigate the apoptosis rate of single and pooled cumulus cells of cumulus cell-oocyte complexes (COCs), as markers of oocyte quality, prior to intracytoplasmatic sperm injection (ICSI Contribution/ OriginalityThis study is one of very few studies which have investigated the apoptosis rate by TUNEL assay and caspase-3 immunoassay of single and pooled human cumulus cells of COCs. The apoptosis rate could be a marker for the selection of the best oocytes to be fertilized by intracytoplasmatic sperm injection.

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“…GC apoptosis and/or necrosis may influence oocyte quality and future embryo development. Studies have reported a positive correlation of GC number with poor IVF/ICSI results [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Effect of GnRH down-regulation on cumulus cell viability and apoptosis as measured by fluorescence-activated cell sorting

Filho¹,

Ribeiro

Rocha

et al. 2008

J Assist Reprod Genet

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Objective To determine whether gonadotropin releasing hormone (GnRH)-agonist or -antagonist induces higher percentages of cumulus cell apoptosis and if the use of either is detrimental to ART outcomes. Patients Women in a private facility under treatment for IVF had their cumulus cells isolated and analyzed by flow cytometry. Viable, apoptotic, and dead cumulus cell rates related to ovarian stimulation by GnRH-agonist orantagonist were measured and compared with fertilization and implantation rates. Results Treatment with GnRH-agonist produced a greater number of follicles than treatment with GnRH-antagonist. No differences in implantation and pregnancy rates were found. While cumulus cell (CC) apoptosis was positively correlated with estradiol on the day of hCG administration, no significant difference in the percentage of apoptotic cells between treatments was detectable. Additionally, implantation rate and the average follicular estradiol production on the day of hCG administration were no different between treatments.Conclusions GnRH-agonist or -antagonist treatment protocols induce similar levels of apoptosis in CCs and are not detrimental to ART outcomes.

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The incidence of apoptotic bodies in membrana granulosa can predict prognosis of ova from patients participating in in vitro fertilization programs

Cited by 133 publications

References 21 publications

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Apoptotic Analysis of Cumulus Cells for the Selection of Competent Oocytes to Be Fertilized by Intracytoplasmic Sperm Injection (ICSI)

Effect of GnRH down-regulation on cumulus cell viability and apoptosis as measured by fluorescence-activated cell sorting

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