The incidence of Clostridioides difficile and Clostridium perfringens netF -positive strains in diarrheic dogs

Diniz, Amanda Nádia; Coura, Fernanda Morcatti; Rupnik, Maja; Adams, Vicki; Stent, Thomas L.; Rood, Julian I.; Oliveira, Carlos Augusto de; Lobato, Francisco Carlos Faria; Silva, Rodrigo

doi:10.1016/j.anaerobe.2017.12.003

Cited by 32 publications

(16 citation statements)

References 32 publications

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“…The C. perfringens NetF toxin gene was found in 2 dogs (corresponding to 4% of the fecal samples) and therefore we conclude it is unlikely to have played a substantial role in causing diarrhea in the present study population. In previous studies, a higher abundance of C. perfringens , encoding for NetF toxin gene was detected in dogs AHDS, 49,50 but C. perfringens encoding for NetF toxin gene decreased within a few days even without the use of antibiotics 51 . A recent paper showed a prevalence of 48.1% in dogs with acute hemorrhagic diarrhea whereas only 12.1% of the healthy dogs carried the NetF gene 52 …”

Section: Discussionmentioning

confidence: 91%

Effect of amoxicillin‐clavulanic acid on clinical scores, intestinal microbiome, and amoxicillin‐resistant Escherichia coli in dogs with uncomplicated acute diarrhea

Werner

Suchodolski

Straubinger

et al. 2020

Veterinary Internal Medicne

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Section: Discussionmentioning

confidence: 91%

Effect of amoxicillin‐clavulanic acid on clinical scores, intestinal microbiome, and amoxicillin‐resistant Escherichia coli in dogs with uncomplicated acute diarrhea

Werner

Suchodolski

Straubinger

et al. 2020

Veterinary Internal Medicne

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“…Thirty four percent of fecal samples tested in the present investigation had detectable CPnetEF genes. The netF encoding gene has been previously identified in 7.6% of dogs with diarrhea and in 57% of dogs with acute hemorrhagic diarrhea syndrome (49, 50). No Clostridium difficile was detected by PCR in this study.…”

Section: Discussionmentioning

confidence: 99%

“…No Clostridium difficile was detected by PCR in this study. The prevalence is largely unknown with current estimates between 10 and 21% in diarrheal dogs (40, 46, 49).…”

Section: Discussionmentioning

confidence: 99%

A Randomized Double Blinded Placebo-Controlled Clinical Trial of a Probiotic or Metronidazole for Acute Canine Diarrhea

et al. 2019

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Acute diarrhea is a common, often self-limiting, cause of presentation for veterinary care, yet there is a paucity of data on frequently-prescribed treatments. The purpose of this randomized, double blinded placebo-controlled clinical trial was to compare two anecdotally-recommended treatments: a probiotic combination and metronidazole. Sixty dogs without concurrent comorbidities were randomized into three treatment groups. The time to resolution of diarrheal signs was evaluated using owner surveys and fecal scoring charts. Dogs presenting with acute diarrhea achieved acceptable fecal consistency after 3.5 ± 2.2 days when receiving probiotic, 4.6 ± 2.4 days with oral metronidazole, and 4.8 ± 2.9 days with placebo; statistically significant differences were not identified between treatment groups ( p = 0.17). These findings failed to provide evidence for the common use of metronidazole in this cohort of dogs with acute canine diarrhea, and a larger study population would be required to identify a statistically significant effect of probiotics.

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“…Aquatic birds could be contaminated by C. difficile spores from water sources (like nearby rivers) and spreading them to the forest. Regarding dogs, C. difficile has been previously reported with a prevalence varying between 4.8% and 11.9% (Alvarez‐Perez, Blanco, Harmanus, Kuijper, & Garcia, ; Diniz et al, ). Sandboxes used by dogs have been also reported to be contaminated with a C. difficile rate of 60% (12 positive sandboxes out of 20 tested) (Orden et al, ).…”

Section: Discussionmentioning

confidence: 99%

Seasonality ofClostridium difficilein the natural environment

Rodríguez

Bouchafa

Soumillion

et al. 2019

Transbound Emerg Dis

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Clostridium difficile is considered the leading cause of antibiotic‐associated disease worldwide. In the past decade, a large number of studies have focused on identifying the main sources of contamination in order to elucidate the complete life cycle of the infection. Hospitals, animals and retail foods have been considered as potential vectors. However, the prevalence of C. difficile in these types of samples was found to be rather low, suggesting that other contamination routes must exist. This study explores the presence of C. difficile in the natural environment and the seasonal dynamics of the bacterium. C. difficile was isolated from a total of 45 samples out of 112 collected (40.2%) on 56 sampling points. A total of 17 points were positive only during the winter sampling (30.4%), 10 were positive only during the summer sampling (17.9%) and 9 sampling points (16.1%) were positive in both summer sampling and winter sampling. Spore counts in soil samples ranged between 50 and 250 cfu/g for 24.4% of the positive samples, with the highest concentrations detected in samples collected in the forest during winter campaign (200–250 cfu/g). A total of 17 different PCR ribotypes were identified, and 15 of them had the genes coding for toxins A and B. Most of those ribotypes had not previously been found or had been isolated only sporadically (<1% of samples) from hospitals in Belgium. Regarding antimicrobial susceptibility, most of the resistant strains were found during the summer campaign. These findings bear out that C. difficile is present in the natural environment, where the bacterium undergoes seasonal variations.

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The incidence of Clostridioides difficile and Clostridium perfringens netF -positive strains in diarrheic dogs

Cited by 32 publications

References 32 publications

Effect of amoxicillin‐clavulanic acid on clinical scores, intestinal microbiome, and amoxicillin‐resistant Escherichia coli in dogs with uncomplicated acute diarrhea

Effect of amoxicillin‐clavulanic acid on clinical scores, intestinal microbiome, and amoxicillin‐resistant Escherichia coli in dogs with uncomplicated acute diarrhea

A Randomized Double Blinded Placebo-Controlled Clinical Trial of a Probiotic or Metronidazole for Acute Canine Diarrhea

Seasonality ofClostridium difficilein the natural environment

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