The incidence of spontaneous arrhythmias in telemetered beagle dogs, Göttingen Minipigs and Cynomolgus non-human primates: A HESI consortium retrospective analysis

Boulay, Emmanuel; Miraucourt, Loïs S.; Abernathy, Matthew M.; Chui, Ray W.; Dalton, Jill A.; Demers, Marjorie; Dybdal, Noël; Gazaille, Elissa; Greiter‐Wilke, Andrea; Hoffmann, Peter; Huang, Hai; LaDuke, Carrie; Norton, Kevin; Pierson, Jennifer; Reeves, Isabelle; Roche, Brian; Rossman, Eric I.; Schultze, A. Eric; Tang, Hui; Wisialowski, Todd; Authier, Simon

doi:10.1016/j.vascn.2023.107266

Cited by 2 publications

(1 citation statement)

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“…The main consideration of this model is that VA is induced by EP pacing, as the mechanisms behind spontaneously occurring VA might be different. However, a study based on spontaneous nonsustained VT would be challenging as these arrhythmias are rare in most animal models ( 46 , 47 ). Also, the MI is invoked by ligation of a pivotal coronary artery in an otherwise healthy heart.…”

Section: Discussionmentioning

confidence: 99%

A comprehensive protocol combining in vivo and ex vivo electrophysiological experiments in an arrhythmogenic animal model

Nyman,

Stølen,

Johnsen

et al. 2024

American Journal of Physiology-Heart and Circulatory Physiology

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Ventricular arrhythmias contribute significantly to cardiovascular mortality, with coronary artery disease as the predominant underlying cause. Understanding the mechanisms of arrhythmogenesis is essential to identify proarrhythmic factors and develop novel approaches for antiarrhythmic prophylaxis and treatment. Animal models are vital in basic research on cardiac arrhythmias, encompassing molecular, cellular, ex vivo whole heart and in vivo models. Most studies employ either in vivo protocols lacking important information on clinical relevance, or exclusively ex vivo protocols, thereby missing the opportunity to explore underlying mechanisms. Consequently, interpretation may be difficult due to dissimilarities in animal models, interventions, and individual properties across animals. Moreover, proarrhythmic effects observed in vivo are often not replicated in corresponding ex vivo preparations during mechanistic studies. We have established a protocol to perform both an in vivo and ex vivo electrophysiological characterization in an arrhythmogenic rat model with heart failure following myocardial infarction. The same animal is followed throughout the experiment. In vivo methods involve intracardiac programmed electrical stimulation and external defibrillation to terminate sustained ventricular arrhythmia. Ex vivo methods conducted on the Langendorff-perfused heart include an electrophysiological study with optical mapping of regional action potentials, conduction velocities, and dispersion of electrophysiological properties. By exploring the retention of the in vivo proarrhythmic phenotype ex vivo, we aim to examine whether the subsequent ex vivo detailed measurements are relevant to in vivo pathological behavior. This protocol can enhance greater understanding of cardiac arrhythmias by providing a standardized, yet adaptable model for evaluating arrhythmogenicity or antiarrhythmic interventions in cardiac diseases.

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Section: Discussionmentioning

confidence: 99%