The Incorporation of Amino Acids Into Ribonucleic Acid

Schweet, Richard S.; Bovard, Freeman C.; Allen, Esther H.; Glassman, Edward

doi:10.1073/pnas.44.2.173

Cited by 81 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rapid non-enzymatic transfer of tryptophan from the adenyl tryptophan to the phosphate of free AMP as shown by the incorporation of AMP32 into adenyl tryptophan, might be analogous to the transfer of activated amino acids to soluble RNA. [20][21][22][23][24][25][26][27] The present experiments do not indicate whether or not this transfer to free AMP could oc('cur with enzynme-bound adenyl tryptophan.…”

Section: Discussionmentioning

confidence: 63%

Enzymatic Formation of Adenyl Tryptophan: Isolation and Identification

Kingdon

Webster

Davie

1958

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 63%

Enzymatic Formation of Adenyl Tryptophan: Isolation and Identification

Kingdon

Webster

Davie

1958

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

“…It has been proposed that after activation, the amino acids are attached to the end of the chain of the soluble, non-microsomal RNA of the final supernatant which acts as a carrier and transfers them to the mierosomal RNA, presumably the RNA of the attached RNP particles (43,(65)(66)(67)(68)(69)(70). At this latter level it is assumed that the amino acids are linked in proper sequence into newly synthesized proteins.…”

Section: Discussionmentioning

confidence: 99%

“…In a number of exploratory experiments we have tried to find out whether the RNA-amino acid intermediates detected and described in vitro (43,(65)(66)(67)(68)(69)(70), occur also in vivo. To this intent 5 minutes after the intravenous injection of DL-leucine-l-C 14, we have removed the liver and pancreas from both young and adult guinea pigs and isolated the whole proteins and RNA's of these organs.…”

Section: Discussionmentioning

confidence: 99%

A Cytochemical Study on the Pancreas of the Guinea Pig

Siekevitz

Palade

1959

The Journal of Cell Biology

View full text Add to dashboard Cite

Five ribonucteoprotein (RNP) fractions were isolated from the postmitochondrial supernatant of the pancreas of the guinea pig. Two were obtained from the microsomes which, by deoxycholate (DOC) treatment, were subdivided into a DOC-soluble and a DOC-insoluble fraction. The latter was taken to represent attached RNP particles. Two other fractions obtained from the microsomal supernatant supposedly represent free RNP particles existing as such in the cytoplasm, while a third fraction resisted sedimentation for 20 hours at 105,000 g and is considered to be a soluble nucleoprotein. These fractions exhibited different RNA/ protein ratios and also different RNA turnover patterns, as determined after in vivo labelling with adenine-8-C 14. However, little discernible differences could be detected in the nucleotide composition of the RNA moieties of these RNP fractions.Amino acid-"activating" enzymes were found to occur in the fraction containing the soluble nucleoproteins.The discussion focuses on the relationships between these fractions and protein synthesis in the pancreas, using data given in this and a previous paper, and data contained in the literature.In a detailed cytochemical investigation of the pancreas of the guinea pig (1-5), we have found that it is possible to separate several cytologically distinct types of nucleoprotein particles (2, 5) derived mostly from the cytoplasm of the exocrine cells of this organ. Since we have already studied (5) the in vivo turnover of the proteins of these particles, we have decided to determine, also in vivo, the turnover of their nucleic acid moiety.In this paper we present our findings on this point together with additional data pertaining to the chemical composition of the particles.The metabolic heterogeneity of the ribonudeic acid (RNA) at cellular and subcellular levels has

show abstract

“…Kikuo Ogata and Hiroyoshi Nohara at the Niigata University School of Medicine in Japan also had collected hints for an RNA-connected intermediate in protein synthesis [88]. By the end of 1957, amino acid-oligonucleotide compounds were being investigated by at least three other research groups: Victor Koningsberger, Olav Van der Grinten, and Johannes Overbeek [89] at the Van't Hoff Laboratory in Utrecht; Richard Schweet, Freeman Bovard, Esther Allen, and Edward Glassman [90] at the Biological Division of Caltech; and Samuel Weiss, George Acs, and Fritz Lipmann [91], who had moved from the Massachusetts General Hospital to the Rockefeller Institute in New York. All of them joined the race for adding items to the list of what these molecules and their activating enzymes did and what they failed to do.…”

Section: Amino Acid Activation and The Emergence Of Soluble Rnamentioning

confidence: 99%

A History of Protein Biosynthesis and Ribosome Research

Rheinberger

2004

Protein Synthesis and Ribosome Structure

View full text Add to dashboard Cite

The Incorporation of Amino Acids Into Ribonucleic Acid

Cited by 81 publications

References 8 publications

Enzymatic Formation of Adenyl Tryptophan: Isolation and Identification

Enzymatic Formation of Adenyl Tryptophan: Isolation and Identification

A Cytochemical Study on the Pancreas of the Guinea Pig

A History of Protein Biosynthesis and Ribosome Research

Contact Info

Product

Resources

About