The incorporation of labeled methionine by typhus rickettsiae

Bovarnick, Marianna R.; Schneider, Louis E.; Walter, Harry

doi:10.1016/0006-3002(59)90131-3

Cited by 37 publications

(41 citation statements)

References 9 publications

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“…It should be pointed out that the additions of ADP and AMP at the concentration indicated were rather inhibitory, even where ATP had been added to the reaction medium, indicating that the phosphorylation of ATP to ADP is predominant in this incorporation. The inhibitory effect of ADP reported here, seems not to be in harmony with the results in typhus rickettsiae previously obtained by Bovarnick (1959Bovarnick ( , 1960b. Further experiments are in progress to elucidate the role of ADP in this system.…”

Section: Effect Of Adenine Nucleotidescontrasting

confidence: 64%

Studies on Metabolism of Rickettsiae Ii. Incorporation of 14c Amino Acid Mixture and 35s Methionine by Rickettsia Mooseri

Kohno¹,

Natsume²,

Shishido³

1961

JJMSB

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Section: Effect Of Adenine Nucleotidescontrasting

confidence: 64%

Studies on Metabolism of Rickettsiae Ii. Incorporation of 14c Amino Acid Mixture and 35s Methionine by Rickettsia Mooseri

Kohno¹,

Natsume²,

Shishido³

1961

JJMSB

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“…The dehydrogenation of glutamine in rickettsia first discovered by the present study may be of special interest in connection with the work of Bovarnick (1959) who has shown that glutamine was utilized as energy source in the incorporation of methionine into R. prowazeki.…”

Section: Discussionmentioning

confidence: 52%

Studies on Metabolism of Rickettsiae I. Studies on Dehydrogenases of Rickettsia Mooseri

Kohno¹

1959

JJMSB

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Since a method was established by Wisseman et al. (1951) for obtaining rickettsiae in a purified state almost free from contamination of yolk sac material, a considerable amount of information has been accumulated concerning the metabolic activities of this group of parasites.The oxidation of various respiratory substrates was discovered in Rickettsia prowazeki and R. mooseri (Bovarnick and Snyder, 1949;Bovarnick and Miller, 1950;Wisseman et al., 1951 Wisseman et al., , 1952. The transamination and oxidative phosphorylation were demonst rated in the above mentioned typhus rickettsiae and in Rickettsia rickettsi (Bovarnick, 1956;Hopps et al., 1956;Price, 1953). Recently, Hays et al. (1957) have demonstrated the oxido-reductive activity of the cytochrome system in R. mooseri by the aid of the sensitive spectrophotometric method originally developed by Chance.Among the numerous difficulties encountered in this line of approach, the most serious are perhaps the extremely poor yield usually encountered in the purification of rikettsiae, and the fast unavoidable contamination of yolk sac components in the final rickettsial preparation. These difficulties are indeed doubled by the circumstance that the available methods of handling rikettsiae are very limited, because of their fragility towards some of the procedures ordinarily adopted in preparation of biological materials.In the present study, an attempt was made to improve the purification method, using an ion-exchange resin instead of celite to remove yolk sac materials (Yamamoto and Kawamura, 1958). A colorimetric method was also developed to determine the dehydro genase activities with as small quantities as possible of purified rickettsiae thus obtained.The presence of various dehydrogenases in R. mooseri were revealed by this method, with 2, 6-dichlorophenol indophenol (2,6 DPIP) as hydrogen acceptor. The effects of various poisons and the immune sera on the dehydrogenase activity were also investigated. The experimental results obtained will be briefly described in the following.

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“…This has been found to be the case in other invertebrates including ticks (Brinton & Burgdorfer, 1971) and tsetse flies (Reinhardt et al, 1972). Rickettsias are said to be energy parasites (Weiss, 1973) and they require exogenous ATP for protein and lipid synthesis (Bovarnick et al, 1959;Bovarnick, 1960). As muscle cells are rich in ATP and other metabolites it seems likely that the RLO reproduce more rapidly in them, than in other tissues.…”

Section: Muscle Tissuesmentioning

confidence: 99%

The distribution and effect of intracellular rickettsia‐ like micro‐ organisms infecting second‐ stage juveniles of the Potato cyst‐ nematode Globodera rostocbiensis

1983

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For the first time rickettsia‐like micro‐organisms are illustrated infecting most tissues of second‐stage juveniles of Globoderu rostochiensis. They were seen only inside host cells, except for the few degenerating forms in the intestinal lumen. They were most common in the non‐contractile (sarcoplasmic) region of body wall muscles. A study of the oesophageal glands of the nematode suggested that micro‐organisms present within these glands would be unlikely to pass out of them and that the nematode would not act as a vector of these micro‐organisms to its host plants. No micro‐organisms were found in the genital primordia of the nematodes. Stored, hatched juveniles appeared to contain more microorganisms than newly hatched juveniles, indicating an increase in the intensity of the infection subsequent to hatching. No obvious signs of pathogenicity were observed in the nematodes. Uninfected juveniles apparently live longer than infected ones in viro, probably because infected juveniles exhaust their lipid reserves sooner than uninfected juveniles. The possible effect of the micro‐organisms on their nematode hosts and their potential as biological control agents are discussed.

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The incorporation of labeled methionine by typhus rickettsiae

Cited by 37 publications

References 9 publications

Studies on Metabolism of Rickettsiae Ii. Incorporation of 14c Amino Acid Mixture and 35s Methionine by Rickettsia Mooseri

Studies on Metabolism of Rickettsiae Ii. Incorporation of 14c Amino Acid Mixture and 35s Methionine by Rickettsia Mooseri

Studies on Metabolism of Rickettsiae I. Studies on Dehydrogenases of Rickettsia Mooseri

The distribution and effect of intracellular rickettsia‐ like micro‐ organisms infecting second‐ stage juveniles of the Potato cyst‐ nematode Globodera rostocbiensis

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