The increased prevalence of Vibrio species and the first reporting of Vibrio jasicida and Vibrio rotiferianus at UK shellfish sites

Harrison, Jamie; Nelson, Kathryn; Morcrette, Helen; Morcrette, Cyril; Preston, Joanne; Helmer, Luke; Titball, Richard W.; Butler, Catherine; Wagley, Sariqa

doi:10.1016/j.watres.2021.117942

Cited by 37 publications

(13 citation statements)

References 79 publications

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“…Antimicrobial susceptibility assay of Vibrio isolates revealed that out of twenty isolates, thirteen (65%) and nine (45%) isolates were found to be ampicillin-resistant and cephalexin-resistant, respectively ( Figure 2 B and Supplementary Table S2 ). Those thirteen isolates were from V. proteolyticus [ 6 ], V. campbellii [ 2 ], V. cincinnatiensis [ 3 ], and V. nereis [ 2 ]. The nine isolates were from V. proteolyticus [ 5 ], V. campbellii [ 2 ], V. cincinnatiensis [ 1 ], and V. nereis [ 1 ].…”

Section: Discussionmentioning

confidence: 99%

“…It has been observed that environmental factors affect both eukaryotic and prokaryotic life through direct or indirect interaction. Jamie et al mentioned in their study that warm sea surface influences the growth of pathogenic Vibrio species which increases the occurrence of infections in humans as well as marine animals [ 6 ]. Tropical countries such as India and other Caribbean countries are the most favorable place for the growth of toxigenic Vibrio species [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Evidence of Antibiotic Resistance and Virulence Factors in Environmental Isolates of Vibrio Species

2023

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The outbreak of waterborne diseases such as cholera and non-cholera (vibriosis) is continuously increasing in the environment due to fecal and sewage discharge in water sources. Cholera and vibriosis are caused by different species of Vibrio genus which are responsible for acute diarrheal disease and soft tissue damage. Although incidences of cholera and vibriosis have been reported from the Vaishali district of Bihar, India, clinical or environmental strains have not been characterized in this region. Out of fifty environmental water samples, twelve different biochemical test results confirmed the presence of twenty Vibrio isolates. The isolates were found to belong to five different Vibrio species, namely V. proteolyticus, V. campbellii, V. nereis, V. cincinnatiensis, and V. harveyi. From the identified isolates, 65% and 45% isolates were found to be resistant to ampicillin and cephalexin, respectively. Additionally, two isolates were found to be resistant against six and four separately selected antibiotics. Furthermore, virulent hlyA and ompW genes were detected by PCR in two different isolates. Additionally, phage induction was also noticed in two different isolates which carry lysogenic phage in their genome. Overall, the results reported the identification of five different Vibrio species in environmental water samples. The isolates showed multiple antibacterial resistance, phage induction, and virulence gene profile in their genome.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evidence of Antibiotic Resistance and Virulence Factors in Environmental Isolates of Vibrio Species

2023

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“…The diffusion of filter-feeders, such as H. perlevis, in an aquaculture scenario could allow fish a better rearing environment, with lower risks related to the presence of pathogenic bacteria such as vibrios, which are one of the main causes of infections and mass deaths of farmed fish. Furthermore, this would also reduce human health risks and the financial losses resulting from the loss of farmed fish products [78][79][80].…”

Section: Discussionmentioning

confidence: 99%

Bioremediation Capabilities of Hymeniacidon perlevis (Porifera, Demospongiae) in a Land-Based Experimental Fish Farm

Longo

Pierri

Mercurio

et al. 2022

JMSE

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The expansion of aquaculture practices in coastal areas can alter the balance of microbial communities in nearby marine ecosystems with negative impacts on both farmed and natural species, as well as on human health through their consumption. Among marine filter-feeder invertebrates, poriferans are known as effective microbial bioremediators, even though they are currently still underutilized in association with fish mariculture plants. In this study, we investigate the microbial bioremediation capability of the demosponge Hymeniacidon perlevis in an experimental land-based fish farm where this species occurred consistently in the drainage conduit of the wastewater. Microbiological analyses of cultivable vibrios, total culturable bacteria (37 °C), fecal and total coliforms, and fecal enterococci were carried out on the fish farm wastewater in two sampling periods: autumn and spring. The results showed that H. perlevis is able to filter and remove all the considered bacterial groups from the wastewater, including human potential pathogens, in both sampling periods. This finding sustains the hypothesis of H. perlevis use as a bioremediator in land-based aquaculture plants as well.

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“…Vibrio species are among the most common and widespread opportunistic bacteria in aquatic systems (Ina‐Salwany et al, 2019; Sanches‐Fernandes et al, 2022). Rising sea‐surface temperature has increased their prevalence, with the genus labelled as the ‘microbial barometer of climate change’ (Baker‐Austin et al, 2017; Harrison et al, 2022; Vezzulli et al, 2013). Vibrio‐ related infections are increasing worldwide, affecting humans and aquatic species alike (Baker‐Austin et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Cold temperature stress and damaged skin induced high mortality in barramundi (Lates calcarifer) challenged with Vibrio harveyi

Samsing

Zhang

Zadoks

et al. 2023

Journal of Fish Diseases

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Most diseases in aquaculture are caused by opportunistic pathogens. One of them, Vibrio harveyi, is a widespread Gram-negative bacterium that has become an important pathogen of aquatic species in marine environments. Here, we propose the use of the causal pie model as a framework to conceptualize the causation of vibriosis in juvenile barramundi (Lates calcarifer) and to establish an effective challenge model. In the model, a sufficient cause, or the causal pie, is a constellation of component causes that lead to an outcome (e.g. vibriosis). In the pilot study, a high cumulative mortality (63.3% ± 10.0%, mean ± SE) was observed when V. harveyi was administered by intraperitoneal injection using a high challenge dose [10 7 colony-forming units (CFU) fish −1 ], but low or no mortality was observed in fish subject to cold stress or fish with intact skin when challenged by immersion. We, therefore, tested the use of a skin lesion (induced with a 4-mm biopsy punch) combined with cold temperature stress to induce vibriosis following the causal pie model. After challenge, fish were immediately subject to cold stress (22°C) or placed at an optimal temperature of 30°C. All groups were challenged with 10 8 CFU mL −1 for 60 min. A considerably higher mortality level (72.7% ± 13.9%) was observed in fish challenged with both a skin lesion and cold stress compared with mortality in fish only having a skin lesion (14.6% ± 2.8%). V. harveyi was re-isolated from all moribund fish and was detected by species-specific real-time PCR in gills, head kidney and liver, regardless of challenge treatment confirming vibriosis as the cause of disease. Parenchymal tissues had histopathological changes consistent with vibriosis. Whole-genome sequence (WGS) is provided for the Vibrio harveyi isolate examined in this study. Overall, the causal pie model was a useful framework to conceptualize the design of the experimental challenge model, in which both cold stress and skin damage were identified as component causes of vibriosis with high mortality. This conceptual framework can be applied to other opportunistic pathogens in aquaculture or to the study of co-infections in fish.

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The increased prevalence of Vibrio species and the first reporting of Vibrio jasicida and Vibrio rotiferianus at UK shellfish sites

Cited by 37 publications

References 79 publications

Evidence of Antibiotic Resistance and Virulence Factors in Environmental Isolates of Vibrio Species

Evidence of Antibiotic Resistance and Virulence Factors in Environmental Isolates of Vibrio Species

Bioremediation Capabilities of Hymeniacidon perlevis (Porifera, Demospongiae) in a Land-Based Experimental Fish Farm

Cold temperature stress and damaged skin induced high mortality in barramundi (Lates calcarifer) challenged with Vibrio harveyi

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