The increasing application of multiplex nucleic acid detection tests to the diagnosis of syndromic infections

Gray, Jim; Coupland, Lindsay

doi:10.1017/s0950268813002367

Cited by 69 publications

(53 citation statements)

References 69 publications

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“…Specimens collected for culture-independent testing may, in some cases, be incompatible with culture because of the collection methods or media that are used for collection. And, a strict reliance on culture-independent diagnostics would limit our ability to detect new causes of diarrheal disease ( 26,(48)(49)(50)(51). Th e future may hold a therapies, and avoidance of antibiotics for viral pathogens or more specifi c anti-microbials for parasitic protozoan etiologies.…”

Section: Diagnosismentioning

confidence: 99%

ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults

Riddle

DuPont

Connor

2016

American Journal of Gastroenterology

328

250

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Acute diarrheal infections are a common health problem globally and among both individuals in the United States and traveling to developing world countries. Multiple modalities including antibiotic and non-antibiotic therapies have been used to address these common infections. Information on treatment, prevention, diagnostics, and the consequences of acute diarrhea infection has emerged and helps to inform clinical management. In this ACG Clinical Guideline, the authors present an evidence-based approach to diagnosis, prevention, and treatment of acute diarrhea infection in both US-based and travel settings. Am J Gastroenterol 2016; 111:602-622; doi: 10.1038/ajg.2016 A follow-up survey where 3,568 respondents (median age 51) were asked at random about illness in the previous 7 days or previous month found that recall bias had an important eff ect on estimates of acute gastrointestinal illness ( 10 ). Using a 7-day exposure window, the estimated incidence of acute diarrhea was 1.6 episodes per person-year, compared with 0.9 episodes per person-year if asked about illness within the preceding month. Other population-based studies from Canada and western European countries using varied methodologies estimate annual incidence between 0.1 to 3.5 episodes per person-year ( 11 18. Antibiotic chemoprophylaxis has moderate to good effectiveness and may be considered in high-risk groups for short-term use. (Strong recommendation, high level of evidence) TD, traveler's diarrhea.

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Section: Diagnosismentioning

confidence: 99%

ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults

Riddle

DuPont

Connor

2016

American Journal of Gastroenterology

328

250

View full text Add to dashboard Cite

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“…It is anticipated that multiplex testing will increase diagnostic positivity, identify co-infections, faster turnaround time and may reduce the use of antibiotics. There are multiplex RT-PCR based laboratory developed test (LDT) and commercial assays (mostly not FDA approved yet) available for gastrointestinal pathogen testing 25, 39 . As more multiplex testing becomes available, the value of using such a test will be assessed and recognized.…”

Section: Detection and Identification Of Gi Pathogens In A Multiplex mentioning

confidence: 99%

Multiplex Polymerase Chain Reaction Tests for Detection of Pathogens Associated with Gastroenteritis

Zhang

Morrison

Tang

2015

Clinics in Laboratory Medicine

152

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Synopsis A wide range of enteric pathogens can cause infectious gastroenteritis. Conventional diagnostic algorithms including culture, biochemical identification, immunoassay and microscopic examination are time consuming and often lack sensitivity and specificity. Advances in molecular technology have as allowed its use as clinical diagnostic tools. Multiplex PCR based testing has made its way to gastroenterology diagnostic arena in recent years. In this article we present a review of recent laboratory developed multiplex PCR tests and current commercial multiplex gastrointestinal pathogen tests. We will focus on two FDA cleared commercial syndromic multiplex tests: Luminex xTAG GPP and Biofire FimArray GI test. These multiplex tests can detect and identify multiple enteric pathogens in one test and provide results within hours. Multiplex PCR tests have shown superior sensitivity to conventional methods for detection of most pathogens. The high negative predictive value of these multiplex tests has led to the suggestion that they be used as screening tools especially in outbreaks. Although the clinical utility and benefit of multiplex PCR test are to be further investigated, implementing these multiplex PCR tests in gastroenterology diagnostic algorithm has the potential to improve diagnosis of infectious gastroenteritis.

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“…Recent studies have demonstrated the test sensitivity range to be between 90 and 100%, depending on the pathogen present, and specificity range between 91 and 99% [27], [28]. The main advantages of this test are the high sample throughput that can be achieved; the number of different analytes detected by the test; and also the ability to determine co-infection in patients [29], [30], [31]. However, a disadvantage of the test is it is not an integrated platform.…”

Section: End Point Pcr – Array Technologiesmentioning

confidence: 99%

A current overview of commercially available nucleic acid diagnostics approaches to detect and identify human gastroenteritis pathogens

Reddington

Tuite

Minogue

et al. 2014

Biomolecular Detection and Quantification

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Purpose of reviewGastroenteritis is caused by a wide range of viral, bacterial and parasitic pathogens and causes millions of deaths worldwide each year, particularly in infant populations in developing countries. Traditional microbiological culture and immunological based tests are time consuming, laborious and often lack diagnostic specificity and sensitivity. As a result patients can receive suboptimal and/or inappropriate antimicrobial treatment. In recent years, rapid nucleic acid diagnostics (NAD) technologies have become available to complement or even bypass and replace these traditional microbiological culture and immunological based tests.The main purpose of this review is to describe a number of recently available multiparametric commercial tests, to support the rapid and accurate clinical diagnosis of human gastroenteritis. These state of the art technologies have the ability to identify a wide range of microorganisms associated with enteric gastroenteritis. Following further technological innovation and more comprehensive clinical validation studies, these NAD tests have the potential to impact on the economic burden of health care systems. These rapid NAD tests can also be used to guide improved patient therapy in a timely manner which will reduce the extent of morbidity and mortality associated with these infections globally.

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The increasing application of multiplex nucleic acid detection tests to the diagnosis of syndromic infections

Cited by 69 publications

References 69 publications

ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults

ACG Clinical Guideline: Diagnosis, Treatment, and Prevention of Acute Diarrheal Infections in Adults

Multiplex Polymerase Chain Reaction Tests for Detection of Pathogens Associated with Gastroenteritis

A current overview of commercially available nucleic acid diagnostics approaches to detect and identify human gastroenteritis pathogens

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