The indirect immunofluorescence assay using cardiac tissue from chickens, quails and ducks for identification of influenza A virus during an outbreak of highly pathogenic avian influenza virus (H5N1): A rapid and simple screening tool for limited resource settings

Antarasena, Chongmas; Sirimujalin, Rungtiva; Prommuang, Porntip; Promkuntod, N.; Prommuang, Praison; Blacksell, Stuart D.

doi:10.1016/j.rvsc.2006.12.007

Cited by 6 publications

(4 citation statements)

References 5 publications

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“…These methods are categorized based on the type of detection target: nucleic acid-based detection, virus isolation and identification, antigen detection, antibody detection, etc. (Alberini et al 2009;Chen et al 2008;Ho et al 2009;Moore et al 2010;Payungporn et al 2006;Xie et al 2006;Yang et al 2008) The most commonly used detection methods are nucleic acid-based detection, such as reverse-transcription PCR, Real-time PCR, RT-LAMP, antibody detection and antigen detection (Antarasena et al 2007;Deng et al 2011;He et al 2007;Kang et al 2010;Khurana et al 2011;Shahsavandi et al 2011;Yea et al 2010;Zhao et al 2010). However, most of these methods require specialized equipment and highly trained operators, and these detection processes are time consuming, thereby making them impractical for point-of-care (POCT) detection (Yager et al 2008 Quantum dots (QDs) have been broadly used for biological labeling and imaging due to their unique optical properties, i.e., a broad excitation spectrum and a narrow and symmetric emission peak (Lu et al 2011;Medintz et al 2005;Zhou et al 2011b).…”

Section: Introductionmentioning

confidence: 99%

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Yuan

Zhou

et al. 2016

Biosensors and Bioelectronics

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A quantum dot-based lateral flow immunoassay system (QD-LFIAS) was developed to simultaneously detect both influenza A virus subtypes H5 and H9. Water-soluble carboxyl-functionalized quantum dots (QDs) were used as fluorescent tags. The QDs were conjugated to specific influenza A virus subtype H5 and H9 antibodies via an amide bond. When influenza A virus subtype H5 or H9 was added to the QD-LFIAS, the QD-labeled antibodies specifically bound to the H5 or H9 subtype viruses and were then captured by the coating antibodies at test line 1 or 2 to form a sandwich complex. This complex produced a bright fluorescent band in response to 365 nm ultraviolet excitation. The intensity of fluorescence can be detected using an inexpensive, low-maintenance instrument, and the virus concentration directly correlates with the fluorescence intensity. The detection limit of the QD-LFIAS for influenza A virus subtype H5 was 0.016 HAU, and the detection limit of the QD-LFIAS for influenza A virus subtype H9 was 0.25 HAU. The specificity and reproducibility were good. The simple analysis step and objective results that can be obtained within 15 min indicate that this QD-LFIAS is a highly efficient test that can be used to monitor and prevent both Influenza A virus subtypes H5 and H9.

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Section: Introductionmentioning

confidence: 99%

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Yuan

Zhou

et al. 2016

Biosensors and Bioelectronics

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“…In addition, there is a growing interest in deploying rapid detection methods in resource-limited settings (Ghosh and Vogt; Hanvoravongchai et al, 2010; Kubo et al, 2010; Ortiz et al, 2009; Oshitani, Kamigaki, and Suzuki, 2008) and expanding non-human influenza surveillance activities (e.g. (Antarasena et al, 2007; Samaan et al, 2011)).…”

Section: Introductionmentioning

confidence: 99%

Rapid, simple influenza RNA extraction from nasopharyngeal samples

Chandler

Griesemer

Cooney

et al. 2012

Journal of Virological Methods

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SUMMARY This report describes the development and pre-clinical testing of a new, random-access RNA sample preparation system (TruTip) for nasopharyngeal samples. The system is based on a monolithic, porous nucleic acid binding matrix embedded within an aerosol-resistant pipette tip and can be operated with single or multi-channel pipettors. Equivalent extraction efficiencies were obtained between automated QIAcube and manual TruTip methods at 106 gene copies influenza A per mL nasopharyngeal aspirate. Influenza A and B amended into nasopharyngeal swabs (in viral transport medium) were detected by real-time RT-PCR at approximately 745 and 370 gene copies per extraction, respectively. RNA extraction efficiency in nasopharyngeal swabs was also comparable to that obtained on an automated QIAcube instrument over a range of input concentrations; the correlation between threshold cycles (or nucleic acid recovery) for TruTip and QIAcube-purified RNA was R2 > 0.99. Preclinical testing of TruTip on blinded nasopharyngeal swab samples resulted in 98% detection accuracy relative to a clinically validated easyMAG extraction method. The physical properties of the TruTip binding matrix and ability to customize its shape and dimensions likewise make it amenable to automation and/or fluidic integration.

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“…This indicates that SP 9 -sCP 1 destroys the NDV structure. Immunofluorescence assays are widely used in antiviral research, and strong fluorescence indicates high antigen expression [ 20 – 22 ]. The results of the present study show that the fluorescence in the SP 9 -sCP 1 treatment group was significantly reduced.…”

Section: Discussionmentioning

confidence: 99%

Solomonseal Polysaccharide and Sulfated Codonopsis pilosula Polysaccharide Synergistically Resist Newcastle Disease Virus

Liu

Chen

et al. 2015

PLoS ONE

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Five combinations of three ratios (PS9-sPS1, PS7-sPS3 and PS6-sPS4) were prepared with polysaccharide (PS) and sulfated polysaccharide (sPS). The antiviral activities of these compounds were subsequently compared in vitro using the MTT assay, observation of the virus structure and immunofluorescence. The results demonstrated that SP9-sCP1, CP7-sCA3, EP7-sAP3, CA9-sEP1 and EP7-sCA3 presented higher activities, and SP9-sCP1 displayed the highest virus inhibition rate and clearly killed the virus and inhibited viral antigen expression. In an in vivo test, 28-day-old chickens were challenged with Newcastle disease virus (NDV) and were administered the five drug combinations. On day 14 after the challenge, the morbidity, mortality and cure rate in each group were calculated. The results indicated that SP9-sCP1 presented the lowest morbidity and mortality and the highest cure rate. These results indicate that Solomonseal polysaccharide and sulfated Codonopsis pilosula polysaccharide synergistically resist NDV. Moreover, SP9-sCP1 had the highest efficacy and may be used as a new antiviral drug.

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The indirect immunofluorescence assay using cardiac tissue from chickens, quails and ducks for identification of influenza A virus during an outbreak of highly pathogenic avian influenza virus (H5N1): A rapid and simple screening tool for limited resource settings

Cited by 6 publications

References 5 publications

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Rapid, simple influenza RNA extraction from nasopharyngeal samples

Solomonseal Polysaccharide and Sulfated Codonopsis pilosula Polysaccharide Synergistically Resist Newcastle Disease Virus

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