The induction of a high resistance to 3‐(3,4‐dichlorophenyl‐1,1‐dimethylurea (DCMU) of oxygen evolution in spinach chloroplasts by trypsin treatment

Ренгер, Г.

doi:10.1016/0014-5793(76)80692-8

Cited by 28 publications

(4 citation statements)

References 21 publications

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“…3 a second order rate constant of kA--3.0" 105 M-a's -1 is obtained for the oxidation of QA" by exogenous K3[Fe(CN)6]. This value is comparable with that obtained previously for trypsin treated thylakoids [27]. After characterization of the functional properties it remains to be shown whether or not the non heme iron is really removed from PS II by the 'iron depletion' procedure.…”

Section: Resultssupporting

confidence: 75%

“…It leads in addition to the fluorescence quenching to an interruption of the electron transfer from Q2" to QB (Q~') and a drastic increase of the susceptibility of QA" towards direct oxidation by K3[Fe(CN)6] [26]. The kinetics of the latter reaction was shown to be strongly dependent on the K3[Fe(CN)a]-concentration [27]. K3[Fe(CN)6] the relaxation kinetics are at least biphasic (squares) and almost invariant to DCMU (circles) as expected for samples with a completely blocked electron transfer from QA" to QB (QB°).…”

Section: Resultsmentioning

confidence: 99%

“…Fig. 3 [27]. After characterization of the functional properties it remains to be shown whether or not the non heme iron is really removed from PS II by the 'iron depletion' procedure.…”

Section: M6ssbauer Measurementsmentioning

confidence: 99%

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Isolation and properties of PS II membrane fragments depleted of the non heme iron center

et al. 1996

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Section: Resultssupporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

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Isolation and properties of PS II membrane fragments depleted of the non heme iron center

et al. 1996

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“…Trypsin treatm ent was found to in te rru p t Q \ reoxidation in the sam e m anner as D C M U in n o r mal chloroplasts [6 ], to decrease the b in d in g o f D CM U -type inhibitors [7] and, concom itantly, to increase the accessibility o f QX to K3[F e (C N )6] [8 ].…”

Section: Bereitgestellt Von | Technische Universität Berlinmentioning

confidence: 99%

Studies on the Functional Mechanism of System II Herbicides in Isolated Chloroplasts

Ренгер

Hagemann

Vermaas

1984

Zeitschrift Für Naturforschung C

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The effect of specific proteolytic enzymes on variable fluorescence, p-benzoquinone-mediated oxygen evolution, PS II herbicide (atrazine and bromoxynil) binding, and protein degradation has been analyzed in isolated class II pea chloroplasts. It was found that: 1. Trypsin and a lysine-specific protease effectively reduce the maximum chlorophyll-a fluorescence yield, whereas the initial fluorescence remains almost constant. At the same number of enzymatic activity units both proteases have practically the same effect. 2. Trypsin and a lysine-specific protease inhibit the p-benzoquinone-mediated flash-induced oxygen evolution with trypsin being markedly more effective at the same number of activity units of both enzymes. Unstacked thylakoids exhibit a higher sensitivity to proteolytic degradation by both enzymes. 3. Trypsin and a lysine-specific protease reduce the binding capacity of [14C]atrazine, but enhance that of [14C]bromoxynil (at long incubation times trypsin treatment also impairs bromoxynil binding). At the same specific activity a markedly longer treatment is required for the lysine-specific protease in order to achieve the same degree of modification as with trypsin. 4. Trypsin was found to attack the rapidly-turned-over 32 kDa-protein severely, whereas the lysine-specific protease does not modify this polypeptide. On the other hand, the lysine-specific protease attacks the light harvesting complex II. 5. Under our experimental conditions an arginine-specific protease did not affect chlorophyll-a fluorescence yield, p-benzoquinone-mediated oxygen evolution, herbicide binding and the poly- peptide pattern. Based on these results a mechanism is proposed in which an as yet unidentified polypeptide with exposable lysine residues, as well as the lysine-free “QB-protein” regulate the electron transfer from Q-A to QB and are involved in herbicide binding.

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The Structural and Functional Organization of Water Cleavage in Photosynthesis

Ренгер

Eckert

Weiß

et al. 1985

Water and Ions in Biological Systems

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The induction of a high resistance to 3‐(3,4‐dichlorophenyl‐1,1‐dimethylurea (DCMU) of oxygen evolution in spinach chloroplasts by trypsin treatment

Cited by 28 publications

References 21 publications

Isolation and properties of PS II membrane fragments depleted of the non heme iron center

Isolation and properties of PS II membrane fragments depleted of the non heme iron center

Studies on the Functional Mechanism of System II Herbicides in Isolated Chloroplasts

The Structural and Functional Organization of Water Cleavage in Photosynthesis

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