The Induction of Myeloid Leukaemias by Rauscher Murine Leukaemia Virus

Both, N.J. de; Stoof, T.J.; Kranendonk, Mariëtte E.G.; Stoker, K.; Vonk, W. P.; Mol, J.G.J.

doi:10.1099/0022-1317-66-4-909

Cited by 7 publications

(7 citation statements)

References 19 publications

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“…In contrast to the Friend virus complex, where both SFFV and MuLV components can induce rapid erythroleukaemia (Troxler & Scolnick, 1978), the Rauscher virus components have a different tissue tropism. R-SFFV always causes acute erythroid disease, whereas RMuLV generally causes lymphoid and myeloid leukaemia (de Both et al, 1985). This difference in target cell specificity cannot be deduced from differences in the LTR.…”

Section: Short Communicationmentioning

confidence: 99%

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Nucleotide Sequence of the Rauscher Murine Leukaemia Virus Long Terminal Repeat

Feltz

Kranendonk-Odijk

Stark

et al. 1986

Journal of General Virology

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Section: Short Communicationmentioning

confidence: 99%

“…R-MuLV is an ecotropic replication-competent virus which predominantly causes chronic lymphoid leukaemia but sometimes causes myeloid leukaemia (de Both et al, 1985). R-MCFV is dualtropic, replication-competent and responsible for a slow erythroleukaemia (Van Griensven & Vogt, 1980).…”

mentioning

confidence: 99%

Nucleotide Sequence of the Rauscher Murine Leukaemia Virus Long Terminal Repeat

Feltz

Kranendonk-Odijk

Stark

et al. 1986

Journal of General Virology

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“…Subcutaneous tumors were induced after injection of l07 myeloid leukemic cells (RMB-1) which were induced by Rauscher murine leukemia virus (R-MuLV) and which grow permanently in vivo and in vitro [11,12]. Animals with disseminated tumor foci in lungs and hemopoietic organs were obtained 3 days after i.v.…”

Section: Mice and Tumorsmentioning

confidence: 99%

The detection of virally induced tumors by 131I- and 125I-labeled syngeneic monoclonal antibodies

Berends

et al. 1988

Cancer Immunol Immunother

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The binding of the syngeneic monoclonal antibodies IC5F5 and 4D2B4 to Rauscher virus-induced myeloid leukemic (RMB-1) cells was analyzed in vivo in tumor-bearing BALB/c mice. To verify it these antibodies bind specifically to RMB-1 cells, purified antibodies were iodinated with the isotopes 125I and 131I. Mice previously inoculated with tumor cells were injected with these labeled monoclonal antibodies and the plasma clearance and the tissue distribution were determined. The clearance in tumor-bearing animals was faster than in control mice. The tissue distribution was corrected for nonspecific accumulation by scoring for an unrelated antibody. Calculation of a localization index showed that IC5F5 binds at least 4.5 times more specifically to tumor cells than to other tissues. A preferential localization of radioactivity in s.c. tumor tissue was seen in the scanning of animals injected with 131I-labeled antibodies. The most direct proof of specific binding was observed in autoradiograms of animals treated with 125I-labeled antibodies. Small islands of tumor cells in the livers of mice inoculated i.v. had a high density of grains compared to other tissues and also compared to tumor cells in mice treated with unrelated monoclonal antibodies. These results show efficient targeting of these monoclonal antibodies and make immunotherapy of these myeloid leukemic cells possible.

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“…1) and R-MCFV [13] although these both contain an EcoRI site in the env gene. These leukemias were diagnosed on the basis of both pathology and cytology [5]. After transfection [9] the cells were grown in medium containing 300 gg/ml of the antibiotic G 418.…”

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confidence: 99%

“…I III I I I II t I I I , I I I / S S X X S P H Pv BB H B B P C R-MuLV RV-1 III I II I I I I Here we describe the testing of the biological activity of R-MuLV clone 9 by cotransfection of ligated viral DNA insert together with the selection marker pKOneo into NIH 3 T 3 cells and subsequent infection of newborn Balb/c mice. Three leukemias were erythroid and the others were lymphoid, a pattern closely resembling that of R-MuLV (XC +) cloned by endpoint dilution [5,14]. After the cells had become confluent polybrene (8 gg/ml) was added for 6 h, the cells were then refed and the following day the culture fluid was tested for reverse transcriptase activity [ 19].…”

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confidence: 99%