The induction of ornithine decarboxylase and DNA synthesis in rat hepatocytes after a single administration of diethylnitrosamine

Abstract

In primary monolayer culture of adult rat liver cells, the presence of a mixture of hormones stimulates DNA synthesis and proliferation. Under conditions of suboptimal hormone stimulation diethylnitrosamine was found to be effective in stimulation of DNA synthesis. Diethylnitrosamine also influences ornithine decarboxylase activity. The effect of some carcinogens and their structural related non-carcinogenic analogs on ornithine decarboxylase activity in primary cultured rat hepatocytes was studied. The addition of the carcinogens diethylnitrosamine, benzo[a]pyrene, methylmethanesulphonate and ethylmethanesulphonate caused significant increases in ornithine decarboxylase activity. Under the same conditions the non-carcinogenic analogs diethylformamide and pyrene did not show this change in enzyme activity.

Stimulation of Hepatic Polyamine Metabolism Following Intraperitoneal Injection of some Dietary Oils

“…Measurements of acyl-CoA oxidase were carried out as described by Eliassen & Osmundsen (1984). Statistical analysis was carried out using one-way analysis of variance, together with a modified t-test in which confidence limits were determined by the method of Bonferroni (Wallenstein et al 1980). Values of P > 0.05 were taken to indicate nonsignificant differences.…”