The induction of revertants by heavy particles and γ-rays in Salmonella tester strains

Kozubek, Stanislav; Krasavin, E. A.; Amirtayev, K.G.; Tokarova, B.; Soska, I.; Drasil, V.; Bonev, M.

doi:10.1016/0027-5107(89)90082-1

Cited by 18 publications

(3 citation statements)

References 7 publications

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“…Its sensitivity was comparable to that of other bioassays, such as the other SOS bioassays mentioned and the Ames mutatest (Table 2). An example is given for ␥ rays: in the Ames mutatest, the lower limit of detection, i.e., the dose required to double the number of revertants over the spontaneous level, is in the range of 10 to 15 Gy for S. typhimurium TA98 and TA100 and 3 to 4 Gy for strain TA102 (9,12). Similarly low detection limits are given for the SOS chromotest, with a range of 5 to 10 Gy (10,11,29).…”

Section: Discussionmentioning

confidence: 99%

“…The Ames mutatest uses a set of strains of Salmonella typhimurium that revert to histidine prototrophy upon exposure to mutagens of specific mechanisms (1,2). Examples are 9-aminoacridine or diethylsulfate, causing frameshift mutations (15), and N-methyl-NЈ-nitro-N-nitrosoguanidine (MNNG), sodium azide, or methylmethanesulfonate, causing base pair substitutions (18), as well as oxidative compounds or ionizing radiation (12,16). The Ames mutatest was validated in studies using several hundred chemicals (reviewed in reference 20).…”

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confidence: 99%

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A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells

Ptitsyn¹,

Horneck²,

Komova³

et al. 1997

Appl Environ Microbiol

141

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A genetically controlled luminescent bacterial reporter assay, the SOS lux test, was developed for rapid detection of environmental genotoxins. The bioassay is based on the recombinant plasmid pPLS-1, which was constructed as a derivative of pBR322, carrying the promoterless luxCDABFE genes of Photobacterium leiognathi downstream of a truncated cda gene from ColD with a strong SOS promoter. E. coli recA ؉ strains containing this construction are inducible to high levels of light production in the presence of substances or agents that cause damage to the DNA of the cells. The light signal, reflecting the SOS-inducing potency, is recorded from the growing culture within 1 s, and the test results are available within 1 to 2 h. Induction of bioluminescence was demonstrated by treatment of E. coli C600(pPLS-1) with 6 genotoxic chemicals (mitomycin C, N-methyl-N-nitro-N-nitrosoguanidine, nalidixic acid, dimethylsulfate, hydrogen peroxide, and formaldehyde) and with UV and ␥ radiation. A clear dose-response relationship was established for all eight genotoxins. The sensitivity of the SOS lux test is similar to that of other bioassays for genotoxicity or mutagenicity, such as the SOS chromotest, umu test, and Ames mutatest. These results indicate that the SOS lux test is potentially useful for the in situ and continuous detection of genotoxins.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells

Ptitsyn¹,

Horneck²,

Komova³

et al. 1997

Appl Environ Microbiol

141

View full text Add to dashboard Cite

show abstract

“…Since the early 1980s, there have been several trials to monitor the effects of ionizing radiation on microorganisms using bacterial cells including the Ames test [1,2]. Before finally applying these methods, several limitations including detection time, detection methods, and sensitivity must be overcome.…”

Section: Introductionmentioning

confidence: 99%

Detection of radiation effects using recombinant bioluminescent Escherichia coli strains

Min

Lee

Moon

et al. 2000

Radiat Environ Biophys

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Effects of ionizing radiation (0.1-500 Gy) on recombinant Escherichia coli cells containing the stress promoters recA, grpE, or katG, fused to luxCDABE, were characterized by monitoring transcriptional responses reflected by the bioluminescent output. The minimum dose of gamma-irradiation detected by E. coli DPD2794 (recA::luxCDABE) was about 1.5 Gy, while the maximum response was obtained at 200 Gy. The amount of emitted bioluminescence increased proportionally with the gamma-ray doses which were found to elicit a DNA damage response in a range of 1-50 Gy. In addition, the cell growth rate was severely, but transiently, retarded by about 50 Gy. Quantification of the gamma-ray dose may be possible using the recA promoter fusion, since linear enhancement of the bioluminescence emission with increasing gamma-ray dose was observed. Other irradiated strains (50 Gy) responsive to either oxidative stress (DPD2511, katG::luxCDABE) or protein-damaging stress (TV1061, grpE::luxCDABE) did not display an increased bioluminescent output, while DPD2794 irradiated by the same dose of gamma-rays gave a significant bioluminescent output. This indicates that the recA promoter is the one most suitable for developing a biosensor for ionizing radiation.

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Recombinant Escherichia Coli Cells as Biodetector System for Genotoxins

Horneck

Ptitsyn²,

Rettberg

et al. 1998

Biosensors for Environmental Diagnostics

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The induction of revertants by heavy particles and γ-rays in Salmonella tester strains

Cited by 18 publications

References 7 publications

A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells

A biosensor for environmental genotoxin screening based on an SOS lux assay in recombinant Escherichia coli cells

Detection of radiation effects using recombinant bioluminescent Escherichia coli strains

Recombinant Escherichia Coli Cells as Biodetector System for Genotoxins

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