The induction of tumour cell adhesiveness and intercellular junctions by a glycoprotein of rat ascites hepatoma cell surface

Ishimaru, Yasuji; Kudo, Koji; Ishihara, Hideko; Hayashi, Hideo

doi:10.1038/bjc.1976.188

Cited by 13 publications

(3 citation statements)

References 17 publications

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“…As reported earlier (Ishimaru et al, 1975;Ishimaru et al, 1976), a mixed preparation of these 2 factors caused both aggregation (as shown in the form of simple apposition) of rat ascites hepatoma AH109A cells present in a free form in vivo, and adhesiveness of the cells, characterized by gradual development of well-defined junctional complexes (including intermediate junctions, desmosomes and focal tight junctions) during a 24 h incubation. Accordingly, it would be of interest to clarify which type of these factors may be associated with development of junctional complexes mentioned above.…”

supporting

confidence: 69%

“…The unabsorbed APF induced cell aggregation (in the form of simple apposition) and then developed well-defined tripartite junctional complexes, including intermediate junctions, desmosomes and focal tight junctions during a period of 24 h incubation; the electronmicroscopic features closely resembled those induced by a mixture of the unabsorbed and absorbed APFs (Ishimaru et al, 1975(Ishimaru et al, , 1976. On the other hand, the absorbed APF at a similar activity produced simple apposition with no development of intermediate junctions, desmosomes or focal tight junctions.…”

Section: Treatment With Trypsin and Periodatementioning

confidence: 86%

“…The frequency of simple apposition, intermediate junction, desmosome and focal tight junction observed at this stage was in the ratio of 10:7:4:0-2 respectively when counted for 50 cells. These electronmicroscope pictures of cell adhesiveness were essentially indistinguishable from those revealed at 24 h after contact with a mixed preparation of the unabsorbed and absorbed APFs (Ishimaru et al, 1975(Ishimaru et al, , 1976 (Gasic and Galanti, 1966) of purified aggregation factor of siliceous sponge (Muiller et al, 1976) and of porcine thyroid cell extract (Giraud et al, 1974). It is thus assumed that the aggregating effect of the absorbed and unabsorbed APFs may be initiated by interaction of the protein portions in their molecules with some components at the surface of the tumour cells.…”

Section: Treatment With Trypsin and Periodatementioning

confidence: 89%

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Biochemical and morphological comparison of two tumour-cell-aggregation factors from rat ascites hepatoma cells

Hanaoka¹,

Kudo²,

Ishimaru³

et al. 1978

Br J Cancer

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Summary.-Two tumour-cell-aggregation factors, derived from rat ascites hepatoma cells, had different antigenicity; one was not absorbed by immunoadsorbent chromatography with anti-rat serum antibody and the other was. Their activities were both lost by digestion with trypsin, but remained unchanged by oxidation with periodate, suggesting the role of the protein portions in their molecules. The potency of the unabsorbed factor was inhibited specifically by a-methyl-D-mannoside or D-mannose, while that of the absorbed factor was inhibited specifically by N-acetyl-D-glucosamine, suggesting that these carbohydrates may be concerned with the respective receptor structures at the tumour-cell surface. The unabsorbed factor induced not only cell aggregation (as shown in the form of simple apposition) but also cell adhesiveness characterized by development of intermediate junctions, desmosomes and tight junctions, while the absorbed factor produced only simple apposition, suggesting their functional difference.

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supporting

confidence: 69%

Section: Treatment With Trypsin and Periodatementioning

confidence: 86%

Section: Treatment With Trypsin and Periodatementioning

confidence: 89%

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Biochemical and morphological comparison of two tumour-cell-aggregation factors from rat ascites hepatoma cells

Hanaoka¹,

Kudo²,

Ishimaru³

et al. 1978

Br J Cancer

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Desmosomal Proteins

Skerrow

1986

Biology of the Integument

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Cell membrane polarity in rat ascites hepatoma cells

et al. 1985

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As previously described, a cell surface-associated adhesive factor (AF) was separated from differentiated rat ascites hepatoma AH136B cells (forming cell islands in vivo) and highly purified by chromatography. AF induces not only aggregation of dissociated AH136B cells or undifferentiated rat ascites hepatoma AH109A cells (present as free cells in vivo), but also adhesiveness characterized by the development of junctional complexes. The localization of AF on the surfaces of AH136B cell islands was investigated using anti-AF IgG (Fab fragment) coupled to peroxidase. AF was detected in the contact region of the lateral surfaces of the AH136B cells and in the intercellular spaces. In contacted free cell surfaces of AH136B cells. Fluorescence studies revealed that biotin-labeled AF did not bind to the apical surface of AH136B cell islands. These results indicate a distinct difference between apical and lateral surfaces of AH136B cells; neither AF nor binding site for AF were localized on the apical surface of AH136B cells, whereas both were localized on the lateral surface. On the other hand, AH136B cells detached from the cell islands, or during the process of partial dissociation from them, showed the loss of the AF localization and binding site of AF on the surfaces. The results suggest that AH136B cell surfaces may be polarized in terms of the AF localization, and this polarization may be lost after cell dissociation.

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The induction of tumour cell adhesiveness and intercellular junctions by a glycoprotein of rat ascites hepatoma cell surface

Cited by 13 publications

References 17 publications

Biochemical and morphological comparison of two tumour-cell-aggregation factors from rat ascites hepatoma cells

Biochemical and morphological comparison of two tumour-cell-aggregation factors from rat ascites hepatoma cells

Desmosomal Proteins

Cell membrane polarity in rat ascites hepatoma cells

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