The infection process of <i>Colletotrichum graminicola</i> and relative aggressiveness on four turfgrass species

Khan, A S; Hsiang, Tom

doi:10.1139/w03-059

Cited by 36 publications

(25 citation statements)

References 19 publications

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“…Latunde-Dada et al, (1996) reported acervuli sporulation in C. destructivum-cowpea pathosystem 120 hai. On C. graminicola-Agrostis palustris and C. graminicola-Lolium perenne pathosystems the acervuli sporulation appeared within 72 and 96 hai, respectively (Khan and Hsiang, 2003).…”

Section: Discussionmentioning

confidence: 96%

Infection Process of Olive Fruits by Colletotrichum acutatum and the Protective Role of the Cuticle and Epidermis

Gomes¹,

Bacelar²,

Martins-Lopes³

et al. 2012

JAS

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Colletotrichum acutatum is a serious concern for the Portuguese oliviculture and food industry, due to both olive oil yield and quality decrease. While the organism's phytopathogenic potential has been well documented, the pathogen adhesion and colonization process in olives remain poorly understood. This paper reports experiments conducted on C. acutatum-susceptible, C. acutatum-moderately resistant and C. acutatum-resistant olive fruits during infection process, in two consecutively years, to identify the physical differences related to the host cuticle promoting resistance and susceptibility to C. acutatum. Cuticle thickness, perimeter and area of epidermal cells of 'Galega' (susceptible), 'Cobrançosa' (moderate-resistant) and 'Picual' (resistant) fruits were measured. Here, we also describe the colonization process of olive fruits by C. acutatum. To achieve this goal olive fruits 102 from 'Galega', 'Cobrançosa' and 'Picual' were inoculated in a field trial with an aqueous suspension of C. acutatum (1x10 6 spores ml -1 ) under suitable humidity and temperature conditions. Light, fluorescent and scanning electron microscopy were used to view olive fruit sections. Significant differences were observed among the parameters studied: cuticle thickness, perimeter and area of olive fruit epidermal cells. The C. acutatum-resistant ('Picual') fruits showed highest mean values for each parameter in both years. Pathogen ultrastructures, such as spores, germ tube, and appressoria were clearly observed using different microscopy techniques. Acervuli sporulation was observed only 192 hours after inoculation in C. acutatum-susceptible ('Galega') fruits.

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Section: Discussionmentioning

confidence: 96%

Infection Process of Olive Fruits by Colletotrichum acutatum and the Protective Role of the Cuticle and Epidermis

Gomes¹,

Bacelar²,

Martins-Lopes³

et al. 2012

JAS

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“…Half of each section was directly mounted onto glass slide in a water droplet, covered with a cover slip and observed under light and GFP filters. Sections were subsequently stained with lactophenol cotton blue to verify the presence of the hyphae [31]. The other half was placed on PDA and incubated at 24 °C with 12 h photoperiod for 3 days to further validate the presence of the fungus.…”

Section: Pathogenicity Testing Of Transformants and Microscopymentioning

confidence: 99%

Genetic transformation of Colletotrichum truncatum associated with anthracnose disease of chili by random insertional mutagenesis

Auyong

Ford

Taylor

2011

J. Basic Microbiol.

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An Agrobacterium tumefaciens -mediated transformation (ATMT) system was successfully developed for Colletotrichum truncatum, the causal agent of chili anthracnose. A. tumefaciens carrying a hygromycin phosphotransferase gene (hph) and a green fluorescent protein (gfp) gene was used to transform the conidiospores of two C. truncatum pathotypes F8-3B and BRIP26974. Optimum transformation efficiency was obtained when equal volumes of A. tumefaciens strain AGL1 carrying either pJF1 or pPK2 binary vector was used to transform C. truncatum conidiospores at 10(6) /ml and co-cultivated at 24 °C for three days. Southern blot analysis indicated that 87.5% of the transformants contained randomly inserted, single copies of the T-DNA. Infection and colonisation of chili fruit at the mature red stage with F8-3B-GFP and BRIP26974-GFP confirmed the maintenance of virulence within these transformed pathotypes. In situ studies of infection and colonisation of the susceptible genotype fruit using fluorescent microscopy and transformed isolates of C. truncatum expressing GFP revealed that the pathogen was able to colonise healthy fruit tissue intercellularly in an endophytic manner without producing secondary biotrophic infection structures. The developed transformation system will be used to study the function of pathogenicity genes in C. truncatum using both forward and reverse genetics approaches.

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“…Members of the genus Colletotrichum are known as cosmopolitan plant pathogens (Sreenivasaprasad and Talhinhas 2005), many of which cause anthracnose in several commercially important crops (Byrne et al 1997; Lardner et al 1999; Khan and Hsiang 2003; Horowitz et al 2004; Xiao et al 2004). Literature on the phytopathogenic genus Colletotrichum (Bailey and Jeger 1992; Prusky et al 2000) includes a single report of the species C .…”

Section: Introductionmentioning

confidence: 99%

Entomopathogenic Activity of a Variety of the Fungus,Colletotrichum acutatum, Recovered from the Elongate Hemlock Scale,Fiorinia externa

Marcelino

Gouli

Parker

et al. 2009

Journal of Insect Science

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A fungal epizootic in populations of Fiorinia externa Ferris (Hemiptera: Diaspididae) infesting hemlock trees, Tsuga canadensis (L.) Carrière (Pinales: Pinaceae) in forests of the Northeastern US has been recently detected. The current known distribution of the epizootic spans 36 sites in New York, Pennsylvania, New Jersey and Connecticut. Colletotrichum acutatum Simmonds var. fioriniae Marcelino and Gouli var. nov. inedit. (Phyllachorales: Phyllachoraceae) was the most prevalent fungus recovered from infected scales. Bioassays indicated that this C. acutatum variety is highly pathogenic to F. externa. Mortality rates of >90 and >55% were obtained for F. externa crawlers and settlers, respectively. Significantly lower mortality levels, ≤ 22%, were obtained when three other species of insects were assayed. C. gleosporioides has also been shown to have pathogenic activity towards a scale insect. The data suggest that C. acutatum var. fioriniae from F. externa epizootics in the US, and the previously reported C. gloeosporioides f. sp. ortheziidae causing Orthezia praelonga epizootics in Brazil, may constitute distinct biotypes of Colletotrichum that have attained the ability to infect insects in addition to the commonly reported plant hosts.

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The infection process of Colletotrichum graminicola and relative aggressiveness on four turfgrass species

Cited by 36 publications

References 19 publications

Infection Process of Olive Fruits by Colletotrichum acutatum and the Protective Role of the Cuticle and Epidermis

Infection Process of Olive Fruits by Colletotrichum acutatum and the Protective Role of the Cuticle and Epidermis

Genetic transformation of Colletotrichum truncatum associated with anthracnose disease of chili by random insertional mutagenesis

Entomopathogenic Activity of a Variety of the Fungus,Colletotrichum acutatum, Recovered from the Elongate Hemlock Scale,Fiorinia externa

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