The infection process of two pathogenic races of<i>Colletotrichum truncatum</i>on lentil

Armstrong-Cho, Cheryl; Wang, J.; Wei, Yangdou; Banniza, Sabine

doi:10.1080/07060661.2012.664565

Cited by 16 publications

(16 citation statements)

References 32 publications

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“…Unlike other biotrophic and hemibiotrophic pathogens, such as Ustilago maydis, Blumeria graminis and Magnaporthe oryzae, C. lentis forms a weak plantpathogen interphase characterized by a swift pulling away of the lentil plasma membrane from the biotrophic primary hypha during plasmolysis, indicating that the pathogen employs a unique infection strategy during the quiescent period of infection (Bhadauria et al, 2011). The length of the biotrophic phase varies and can last up to several days depending on temperature and humidity (Armstrong-Cho et al, 2012). The biotrophic phase ceases once the pathogen deploys the effector CtNUDIX, which probably dismantles the integrity of the lentil plasma membrane (Bhadauria et al, 2013a,b).…”

Section: Introductionmentioning

confidence: 99%

“…Races of C. lentis lack the characteristic features of the classical physiological races, such as completely resistant host cultivars and clearly incompatible reactions characterized by the hypersensitive response. Although differences in responses to the races in partially resistant and fully susceptible lentil cultivars are distinct, there is a strong quantitative effect involved in the interactions (Armstrong-Cho et al, 2012). Sources of partial resistance against the less virulent race 1 have been identified in L. culinaris and were successfully introgressed into lentil cultivars, such as L. culinaris spp.…”

Section: Introductionmentioning

confidence: 99%

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Genetic map‐guided genome assembly reveals a virulence‐governing minichromosome in the lentil anthracnose pathogen Colletotrichum lentis

et al. 2018

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Colletotrichum lentis causes anthracnose, which is a serious disease on lentil and can account for up to 70% crop loss. Two pathogenic races, 0 and 1, have been described in the C. lentis population from lentil. To unravel the genetic control of virulence, an isolate of the virulent race 0 was sequenced at 1481-fold genomic coverage. The 56.10-Mb genome assembly consists of 50 scaffolds with N scaffold length of 4.89 Mb. A total of 11 436 protein-coding gene models was predicted in the genome with 237 coding candidate effectors, 43 secondary metabolite biosynthetic enzymes and 229 carbohydrate-active enzymes (CAZymes), suggesting a contraction of the virulence gene repertoire in C. lentis. Scaffolds were assigned to 10 core and two minichromosomes using a population (race 0 × race 1, n = 94 progeny isolates) sequencing-based, high-density (14 312 single nucleotide polymorphisms) genetic map. Composite interval mapping revealed a single quantitative trait locus (QTL), qClVIR-11, located on minichromosome 11, explaining 85% of the variability in virulence of the C. lentis population. The QTL covers a physical distance of 0.84 Mb with 98 genes, including seven candidate effector and two secondary metabolite genes. Taken together, the study provides genetic and physical evidence for the existence of a minichromosome controlling the C. lentis virulence on lentil.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic map‐guided genome assembly reveals a virulence‐governing minichromosome in the lentil anthracnose pathogen Colletotrichum lentis

et al. 2018

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“…For infection assays, fungal spores (conidia) were collected from 10-day-old oatmeal agar cultures by flooding Petri dishes with sterile water. Three-week-old lentil plants and fungal spore suspensions (4 × 10 4 conidia/mL) were used in attached and detached infection assays [4]. …”

Section: Methodsmentioning

confidence: 99%

“…Lentil cultivars Eston and CDC Robin were sprayed with C. truncatum CT-21, and incubated under controlled conditions conducive to anthracnose infection as described elsewhere [4]. Leaf tissues were harvested at 24 hours intervals until 7 dpi, frozen in liquid nitrogen and stored at -80°C until required.…”

Section: Methodsmentioning

confidence: 99%

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Identification of Lens culinaris defense genes responsive to the anthracnose pathogen Colletotrichum truncatum

et al. 2013

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BackgroundAnthracnose of lentil, caused by the hemibiotrophic fungal pathogen Colletotrichum truncatum is a serious threat to lentil production in western Canada. Colletotrichum truncatum employs a bi-phasic infection strategy characterized by initial symptomless biotrophic and subsequent destructive necrotrophic colonization of its host. The transition from biotrophy to necrotrophy (known as the biotrophy-necrotrophy switch [BNS]) is critical in anthracnose development. Understanding plant responses during the BNS is the key to designing a strategy for incorporating resistance against hemibiotrophic pathogens either via introgression of resistance genes or quantitative trait loci contributing to host defense into elite cultivars, or via incorporation of resistance by biotechnological means.ResultsThe in planta BNS of C. truncatum was determined by histochemical analysis of infected lentil leaf tissues in time-course experiments. A total of 2852 lentil expressed sequence tags (ESTs) derived from C. truncatum-infected leaf tissues were analyzed to catalogue defense related genes. These ESTs could be assembled into 1682 unigenes. Of these, 101 unigenes encoded membrane and transport associated proteins, 159 encoded proteins implicated in signal transduction and 387 were predicted to be stress and defense related proteins (GenBank accessions: JG293480 to JG293479). The most abundant class of defense related proteins contained pathogenesis related proteins (encoded by 125 ESTs) followed by heat shock proteins, glutathione S-transferase, protein kinases, protein phosphatase, zinc finger proteins, peroxidase, GTP binding proteins, resistance proteins and syringolide-induced proteins. Quantitative RT-PCR was conducted to compare the expression of two resistance genes of the NBS-LRR class in susceptible and partially resistant genotypes. One (contig186) was induced 6 days post-inoculation (dpi) in a susceptible host genotype (Eston) whereas the mRNA level of another ( LT21-1990) peaked 4 dpi in a partially resistant host genotype (Robin), suggesting roles in conditioning the susceptibility and conferring tolerance to the pathogen, respectively.ConclusionsData obtained in this study suggest that lentil cells recognize C. truncatum at the BNS and in response, mount an inducible defense as evident by a high number of transcripts (23% of the total pathogen-responsive lentil transcriptome) encoding defense related proteins. Temporal expression polymorphism of defense related genes could be used to distinguish the response of a lentil genotype as susceptible or resistant.

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Effect of temperature on Colletotrichum truncatum growth, and evaluation of its inoculum potential in soybean seed germination

et al. 2021

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The infection process of two pathogenic races ofColletotrichum truncatumon lentil

Cited by 16 publications

References 32 publications

Genetic map‐guided genome assembly reveals a virulence‐governing minichromosome in the lentil anthracnose pathogen Colletotrichum lentis

Genetic map‐guided genome assembly reveals a virulence‐governing minichromosome in the lentil anthracnose pathogen Colletotrichum lentis

Identification of Lens culinaris defense genes responsive to the anthracnose pathogen Colletotrichum truncatum

Effect of temperature on Colletotrichum truncatum growth, and evaluation of its inoculum potential in soybean seed germination

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