2022
DOI: 10.3389/fmicb.2022.911751
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The Infection Route of Tomato Zonate Spot Virus in the Digestive System of Its Insect Vector Frankliniella occidentalis

Abstract: Tomato zonate spot virus (TZSV) is a phytopathogen of the genus Orthotospovirus (Bunyaviridae) that is widespread in many areas of Southwest China. TZSV is mainly transmitted by Frankliniella occidentalis, but its exact infection route remains unclear. To explore this issue, we detected the nucleocapsid protein of TZSV in the digestive systems of first-instar F. occidentalis nymphs fed with TZSV-infected pepper leaves. TZSV infection in the F. occidentalis digestive system begins within 4 h post-first access t… Show more

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Cited by 2 publications
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“…Female thrips (2-day-old) antennae were dissected, fixed, dehydrated, and embedded as described previously [ 54 ]. The samples were sectioned using an ultramicrotome (Leica UC5, Germany) and incubated with FintCSP1-specific IgG and immunogold-labeled anti-goat antibodies against rabbit IgG that had been conjugated with 12-nm-diameter gold particles (Jackson).…”
Section: Methodsmentioning
confidence: 99%
“…Female thrips (2-day-old) antennae were dissected, fixed, dehydrated, and embedded as described previously [ 54 ]. The samples were sectioned using an ultramicrotome (Leica UC5, Germany) and incubated with FintCSP1-specific IgG and immunogold-labeled anti-goat antibodies against rabbit IgG that had been conjugated with 12-nm-diameter gold particles (Jackson).…”
Section: Methodsmentioning
confidence: 99%