The inflammatory responses to silk films in vitro and in vivo

Meinel, Lorenz; Hofmann, Sandra; Karageorgiou, Vassilis; Kirker‐Head, Carl A.; McCool, J.; Gronowicz, Gloria; Zichner, Ludwig; Langer, Robert; Vunjak-Novakovic, Gordana; Kaplan, David L.

doi:10.1016/j.biomaterials.2004.02.047

Cited by 740 publications

(530 citation statements)

References 21 publications

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“…Silk fibroin, the structural, hydrophobic, and approximately 370 kDa protein isolated from the cocoon of B. mori [43][44][45][46] that has emerged as a novel substrate for engineering biomaterials, has proven to be biocompatible [22,33], offers distinguishing mechanical properties when compared to other naturally derived or degradable polymeric biomaterials [47], and exhibits low inflammatory response [21,22].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Silk based biomaterials to heal critical sized femur defects

Meinel

Betz

Fajardo

et al. 2006

Bone

221

179

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Section: Discussionmentioning

confidence: 99%

“…These scaffolds have low immunogenicity when properly separated from immunogenic and glycosylated proteins [19][20][21][22]. Furthermore, they can be produced in a variety of formats including electrospun nets, three-dimensional scaffolds, or tendon-like structures [23][24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Silk based biomaterials to heal critical sized femur defects

Meinel

Betz

Fajardo

et al. 2006

Bone

221

179

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“…The biocompatibility of implant materials is of major concern in orthopedic surgery. [18][19][20][21] After implantation, hostmaterial interactions occurring at the site of implantation can cause adverse inflammatory reactions. All of the biomaterials derived from a nonautologous source will cause some level of foreign body response after implantation in vivo.…”

Section: Evaluation Of Biocompatibility In Vitromentioning

confidence: 99%

Increase in cell migration and angiogenesis in a composite silk scaffold for tissue‐engineered ligaments

Seo

Yoon

Song

et al. 2009

Journal Orthopaedic Research

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The purpose of this study was to evaluate the biocompatibility of silk and collagen-hyaluronan (HA) in vitro by assessing anterior cruciate ligament (ACL) cell and T-lymphocyte cultures on scaffolds. The use of composite scaffolds as artificial ligaments in ACL reconstruction and their effects on angiogenesis were evaluated in vivo. The silk scaffold was knitted by hand and dry coated with collagen-HA, whereas the composite silk scaffold was made by covering a silk scaffold with a lyophilized collagen-HA substrate. The initial attachment and proliferation of human ACL cells on the composite silk scaffold was superior to the attachment and proliferation observed on the silk scaffold. The immune response was higher in both scaffolds after 72 h ( p < 0.05) compared with the control culture condition without scaffolding, as assessed by T-lymphocyte cultures in vitro. There was no significant difference in the immune response in vitro between the silk and composite silk scaffolds. Silk and composite silk scaffolds were implanted as artificial ligaments in ACLs removed from the knees of dogs, and they were harvested 6 weeks after implantation. On gross examination, the onset of an inflammatory tissue reaction, such as synovitis, was seen in both the silk scaffold and the composite silk scaffold groups. An histological evaluation of the artificial ligament implants revealed the presence of monocytes in the silk composite scaffold and the absence of giant cells in all cases. MT staining in the composite silk scaffold-grafted group showed granulation tissue consisting of fibroblasts, lymphocytes, monocytes, and collagen fibers. In addition, CD31 staining revealed the formation of new blood vessels. On the other hand, no reparative tissues, such as blood vessels, collagen, and cells, were observed in the silk scaffold-grafted group. These results suggest that the lyophilized collagen-HA substrate is biocompatible in vitro and enhances new blood vessel and cell migration in vivo. ß

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“…Especially, fibroin as the main component of silk protein has found diverse applications in the biomedical field, which has a strong tensile strength, biodegradability and biocompatibility (Horan et al, 2005;Meinel et al, 2005). Hence, many researchers select silk fibroin as one of the materials for biomedical application.…”

Section: Introductionmentioning

confidence: 99%

“…Silk fibroin is a natural fibrous protein with a semicrystalline structure and it consists of heavy chain (390 kDa), light chain (25 kDa) and glycoprotein. Each polypeptides linked by disulfide bond (Zhou et al, 2001).Especially, fibroin as the main component of silk protein has found diverse applications in the biomedical field, which has a strong tensile strength, biodegradability and biocompatibility (Horan et al, 2005;Meinel et al, 2005). Hence, many researchers select silk fibroin as one of the materials for biomedical application.…”

mentioning

confidence: 99%

Cytocompatibility of silkworm cocoon layer extracts

Jo¹,

Kim²,

Lee³

et al. 2016

International Journal of Industrial Entomology

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Recently silk polymer produced by Bombyx mori silkworm has been considered as biological macromolecules. Silk polymer was extracted in PBS solution at 37 o C for 72 h or 72 o C for 24 h. The effect of EtOH treatment on the cocoon extraction was also examined. The extraction yield of cocoon was less than 1 wt% regardless of extraction conditions. UV spectroscopy showed that the experimental extracts have absorption bands at 280 nm. There is no cytotoxicity effect on the mouse fibroblast L929 cell. The phenotype of L929 cell was not changed under the experimental conditions. The proliferation behavior of L929 cell was not affected by the addition of cocoon extract. Therefore, cocoon extract might be cytocompatible and can be used as promising biomaterials. IntroductionA silkworm cocoon of Bombyx mori provides silk fiber for textile industries for more than 4,000 years. Recently, silk polymer has been revealed several advantages for biological materials and then applied various functional materials such as cosmetics, suture and artificial eardrum (Kundu et al., 2013;Ju et al., 2014;Vepari and Kaplan, 2007; Kim et al., 2010), and so on.In the view point of material, silk is a natural protein polymer composed of two proteins: one is a central protein called silk fibroin and another is gum-like protein called silk sericin. Silk fibroin is a natural fibrous protein with a semicrystalline structure and it consists of heavy chain (390 kDa), light chain (25 kDa) and glycoprotein. Each polypeptides linked by disulfide bond (Zhou et al., 2001).Especially, fibroin as the main component of silk protein has found diverse applications in the biomedical field, which has a strong tensile strength, biodegradability and biocompatibility (Horan et al., 2005;Meinel et al., 2005). Hence, many researchers select silk fibroin as one of the materials for biomedical application.Silk sericin is a protein polymer, which acts as an adhesive binder to maintain the structure of the fibers. Sericin is an albuminoid protein composed of serine (30%) and other amino acids (Teramoto and Miyazawa, 2005;Garel et al., 1997). Sericin was removed easily from Bombyx mori cocoon using a thermo or chemical process called degumming (Mo et al., 2009) Preparation of cocoon extractsEach cocoon was washed with tap water and then dried absolutely with room temperature in the dark. The cocoon was sliced into 5 mm ⅹ 5 mm pieces. Each cocoon slices were divided into two groups; one group was treated with absolute ethyl alcohol (EtOH) for 24 h at R.T. and another group was not treated EtOH. After treatment, cocoon was dried with R.T. for overnight. The extraction condition were followed; weigh 1 g of cocoon and add into 10 mL of 1X PBS buffer at 37 o C for 72 h and/or 72 o C for 24 h. Save the cocoon extracts and then filtered it with 0.22 um syringe filter (Sartorius Ltd., Epsom, UK) and protein concentration of all extracts was determined by bicinchronic acid (BCA) assay. Forty ug of each extracts used for cell cytotoxicity and proliferation assay. UV vis sp...

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The inflammatory responses to silk films in vitro and in vivo

Cited by 740 publications

References 21 publications

Silk based biomaterials to heal critical sized femur defects

Silk based biomaterials to heal critical sized femur defects

Increase in cell migration and angiogenesis in a composite silk scaffold for tissue‐engineered ligaments

Cytocompatibility of silkworm cocoon layer extracts

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