The influence of 3‐glycidyloxypropyl trimethoxysilane on the rheological and in‐vitro behavior of injectable composites containing64Sbioactive glass, chitosan, and gelatin

Abstract

The effect of cross‐linker 3‐glycidyloxypropyl trimethoxysilane (GPTMS) on the injectability, bioactivity, rheology, washout resistance, and cellular behavior of bio‐nano composite pastes containing a sol–gel derived bioactive glass (BG), chitosan (Cn), and gelatin (Gel), were studied. The results indicated that the structural integrity of the samples in the stimulated body fluid (SBF) was improved from 80% to 90% with the increase in the GPTMS content. In addition, the viscosity and injectability of the paste were affected, so that the later changed from 96% to 73% and the former changed from 132 × 103 to 210 × 103 Pa.s, with addition of 20 wt% GPTMS. Furthermore, the bioactivity of the pastes was improved with the use of GPTMS. No toxicity was observed in the cellular studies of the GPTMS containing paste after 7 days, and the cells kept their shape and were attached firmly to the paste surface. The results of the present work showed that the use of GPTMS was beneficial in enhancing the properties of injectable paste.

Silane Grafted Biosourced Melanin: A Sustainable Approach for Nanobiosensing Applications

“…It is well established that a release of silicon ions can increase the gene expression, the proliferation and differentiation of cells, as well as the osteogenesis process. Therefore, since it was already proved that Si ions are released from the MNPs GPTMS, the results obtained were as expected …”

“…Therefore, since it was already proved that Si ions are released from the MNPs GPTMS, the results obtained were as expected. 77 SaOs-2 cytoskeleton was analyzed by phalloidin staining (Figure 6(a)) after 72 h of culture with MNPs GPTMS. Independent of particle concentration, cells proliferated and showed a spread morphology.…”

Antioxidant flavonoid-loaded nano-bioactive glass bone paste: in vitro apatite formation and flow behavior

“…53,54 To generate a layer of hydroxycarbonate apatite (HCA), which is identical to the hydroxyapatite found naturally in bone, on the surface of these biomaterials, contact with body fluids is suggested for bone-bonding creation. 51–53,55…”

“…53,54 To generate a layer of hydroxycarbonate apatite (HCA), which is identical to the hydroxyapatite found naturally in bone, on the surface of these biomaterials, contact with body uids is suggested for bone-bonding creation. [51][52][53]55 Signs of a poorly crystalline apatite phase as a broad diffraction pattern are observed before the immersion of the pastes in SBF solution. The presence of an apatite phase before immersion in SBF can be due to the dissolution-precipitation phenomenon of the glass particles and apatite within the water phase of the paste.…”

“…The absorption bands at 1630 and 3000-3750 cm −1 were assigned to the OH group. 52,55,56 The signals of the (CO 3 ) 2− group substituted for the (PO 4 ) 3− group in the apatite lattice were found at 870, 1420, and 1460 cm −1 . 8,51,57 When the Q content increased, the single C-O stretching band at 1420 cm −1 changed into a twin (at 1420 and 1460 cm −1 ), while the absorption intensity of the C-O band at 870 cm −1 increased.…”