The Influence of a Combination of Antioxidants on Sub Fertile Male Rat.

Abstract

Background: Prescription of a combination of antioxidants might increase the quality of sperm parameters and decrease the serum level of cholesterol and serum level of triglycerine in men who suffer from infertility. Therefore, the present study investigated protective effects of alpha-Lipoic acid (ALA), L-Carnitine (CAR), N-Acytel cysteine (NAC) on sperm parameters and seminiferous tubules epithelium in high-fat diet model. Objectives: is to investigate the serum cholesterol and its effect on male rat's fertility and evaluate the effect of treating them with a certain combination of antioxidants mixed together they were (ALA), L-(CAR) and N-Acytel (NAC). Material and Methods: thirty male rats were administered cholesterol for 2 months to induce hypercholesterolemia to reduce fertility in male rats and consequently treated with a combination of antioxidants to repair the harmful effect of cholesterol. Results: cholesterol administered group were significantly different with those of control group (P<0.05) cholesterol levels were153.51±5.39 and 93.61±4.68 mg/dl while triglyceride was 176.71±12.49 and 83.14±7.08 mg/dl in treated and control group respectively. Also semen parameters showed significant difference between treated and control group (P<0.05) they were 28.46±3.56 and 74.4±6.36 ×106 sperm/ml, 34.23±2.12 and 89.34±2.41%, 32.45±5.23 and3.76±1.22%, 20.34±3.81 and 3.21±1.08% for sperm count, live sperms, sperm agglutination and abnormal sperm percentage in treated and control groups respectively. While after antioxidant administration showed significant difference (P<0.05) with the control group in sperm count, bigger live sperms percentage and lesser abnormal sperms was seen in combination of three antioxidants were (P<0.05) compared to positive control group (non-antioxidant group), sperm agglutination had a significant difference (P<0.05) between all antioxidant treated group compared with positive control group (non-treated group). Conclusion: As it is shown in our study, Oral administration of a combination of antioxidants with diet, decreasing the serum levels of cholesterol and serum level of TG, improving the sperm parameters by decreasing the ROS and protect the liver tissue from the harmful effect of cholesterol. Finally, it seems that the antioxidants in patients with subfertility or infertility is a new and efficient strategy with few side effects.