The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803

Abstract

Background: The dspA (hik33) gene, coding for a putative sensory histidine kinase, is conserved in plastids (ycf26) and cyanobacteria. It has been linked with a number of different stress responses in cyanobacteria.

Effects of Quantum Dots Adsorption on Algal Photosynthesis

“…A fluorescence spectrophotometer (Varian Cary Eclipse) was used to examine the effects of QDs fluorescence on the photo properties of algal cells. The excitation was set at 435 nm, a wavelength known to induce strong light absorption by the chlorophyll in algae. , Fluorescence spectra were recorded for algae, QDs (50 ppm), and algae/QDs solution (QDs: 50 ppm; incubation time: 2 h) within the wavelength range of 500−800 nm, at 1 nm/step.…”

Synthetic Biology and Metabolic Engineering Approaches To Produce Biofuels

“…Six genes were deleted from the Synechocystis genome: The gene slr1609 , which codes for an acyl-ACP synthetase (homologous to fadD in E. coli); sll1951 , which codes for a hemolysin-like surface layer protein with function in cell membrane stability; slr1710 , a gene with a role in peptidoglycan assembly; pta , which codes for a phosphotransacetylase; and two cyanophycin synthetase genes to avoid formation of the chemical storage molecule cyanophycin . Modifications were maintained in various combinations in a total of 18 strains.…”

Chloroplast two-component systems: evolution of the link between photosynthesis and gene expression

“…In these two species, ycf26 is likely to be a soluble stromal protein with only the PAS sensor domain and the kinase domain. It appears that in most ycf26 proteins the predicted lumenal loop and the internal PAS domain act as two separate sensor domains (Morrison et al 2005).…”

“…PCC 6803, histidine kinases 33 and 2 (hik33 and hik2) are homologues of ycf26 and CSK, respectively (Ashby & Houmard 2006;Puthiyaveetil et al 2008). hik33 is also known as 'drug sensory protein A' (dspA; Hsiao et al 2004;Morrison et al 2005) in Synechocystis sp. PCC 6803 and as NblS in Synechococcus elongatus PCC 7942.…”

“…This multifunctional role could easily qualify hik33 as a global regulator in cyanobacteria. With its lumenal loop and PAS domain acting as two separate sensor domains, hik33 will be able to combine or integrate multiple signals and act as a multi-sensor (Morrison et al 2005). Environmental factors such as low temperature, nutrient stress and osmotic stress may slow down photosynthetic electron transport, generating redox signals that will themselves feed into the regulatory loop of hik33.…”