The influence of age on Na-K-ATP-ase activity in erythrocytes in fetal and newborn guinea pigs

Rosén, Karl G.; Sigström, L

doi:10.1515/jpme.1978.6.3.154

Cited by 9 publications

(10 citation statements)

References 12 publications

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“…In agreement with our findings, most previous studies have also found higher erythrocyte Na + ,K + -ATPase activity6 10 11 and ouabain binding capacity12 in the immature animal and neonate than in the mature newborn. On the other hand, contrary to our present and the above mentioned previous findings,6 10-12 a recent study by Bistritzer et al 13 found that cord blood erythrocyte ATPase activity is lower in preterm neonates of between 30 and 34 weeks of gestation than neonates of ⩾ 35 weeks gestation.…”

Section: Discussionsupporting

confidence: 93%

“…As for the [K + ] ic , previous studies8 11 have also found that [K + ] ic does not necessarily change with maturation (table 2). However, because of the high [K + ] ic and the unique stoichiometry of the enzyme, it is generally believed that [K + ] ic is a less sensitive marker of Na + ,K + -ATPase cation pump function than [Na + ] ic 10. Finally, as mentioned above, developmentally regulated changes in the expression and/or function of the Na + and K + leak pathways may have contributed to the changes in intracellular cation concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…Development also appears to have an impact on enzyme activity and cation pump function, but the findings are scanty and somewhat contradictory 610-13 Most studies on erythrocyte Na + ,K + -ATPase activity during development have shown higher enzyme activity6 10 11 and ouabain binding capacity12 in the immature animal and neonate than in the mature newborn. However, a recent study found that cord blood erythrocyte Na + ,K + -ATPase activity is lower in preterm than term neonates 13.…”

mentioning

confidence: 99%

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Developmental changes in erythrocyte Na+,K+-ATPase subunit abundance and enzyme activity in neonates

Vásárhelyi

2000

Archives of Disease in Childhood - Fetal and Neonatal Edition

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Developmental changes in erythrocyte Na+,K+-ATPase subunit abundance and enzyme activity in neonates

Vásárhelyi

2000

Archives of Disease in Childhood - Fetal and Neonatal Edition

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“…Marsh and colleagues (1981) also measured ouabain-sensitive myocardial #6frubidium uptake in dogs and guinea-pigs and found this to be significantly higher in young animals. Values Parker & Hoffman, 1967) and it has been suggested that increased cellular Na+K+ ATPase activity is related to the ability of the foetus to withstand hypoxia (Rosen & Sigstrom, 1978).…”

Section: Discussionmentioning

confidence: 99%

The effect of digoxin on 86rubidium uptake by erythrocytes from mothers and babies.

Kelly¹,

McMillen²,

McDevitt³

1983

Brit J Clinical Pharma

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1The uptake of 86rubidium by erythrocytes from mothers and babies has been used as a model system to investigate possible differences in sensitivity to digoxin in the very young. 2 While total 86#rubidium uptake was not significantly different between mothers and babies, the digoxin-sensitive proportion was higher in neonatal erythrocytes. Neonatal cells were less sensitive to digoxin, denionstrated by the requirement for a larger amount of digoxin to inhibit 816rubidium uptake and this was accompanied by an increase in numbers of specific erythrocyte binding sites for digoxin.3 These results provide further evidence in support of the hypothesis of decreased sensitivity to digoxin in the very young.

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“…No correlation could be found between serum potassium concentrations and fractional excretion of potassium or sodium. DISCUSSION A variety of animal studies has documented less sensitivity of the newborn to digitalis when compared to adults (4,5,12). Achieving either clinical or toxic effects of digitalis requires larger doses of the glycosides in the immature animal.…”

Section: Resultsmentioning

confidence: 99%

Comparison of the Digitalis Receptor in Erythrocytes from Preterm Infants and Adults

et al. 1988

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ABSTRACT. We compared adrubidium by erythrocytesThe membrane Na", K' , ATPase is considered by most auof preterm infants and adults as a measurement of their thorities to be the pharmacologic receptor for digitalis glycosides Na+, K+, ATPase enzyme system. In neonates, total uptake (7). By specifically inhibiting this enzyme, higher intracellular (0.92 2 0.13 pg/106 cells) and specific uptake (0.64 f concentrations of sodium are achieved; exchange of increased 0.076 pg/106 cells) were significantly higher than in adults amounts of sodium with more extracellular calcium results in (0.52 f 0.1 and 0.29 2 0.06 pg/106 cells, respectively; p < increased cardiac ionotrophy (8). However, the mechanism of 0.025). The percentage of specific uptake from total uptake action of digitalis in increasing cardiac inotrophy is still largely was higher in infants (73.3 2 2.3%) than in adults (57.9 f conjectural. 4.6%) ( p < 0.005). No differences were found in the affinityRb is a kaliumimetic cation that moves across cell membranes constant of 86Rb uptake between infants (4.35 f 0.48 ng/ in a fashion similar to potassium (9). Thus, by measuring Rb+ ml) and adults (4.85 2 0.48 nglml). Stratification of infants uptake, one may assess the activity of the membrane Na+, K", according to their serum K+ concentrations revealed that ATPase enzyme both in terms of binding capacity and affinity levels above 5.4 mEq/liter were associated with a higher (10). Rb, rubidiumAll were hospitalized in our neonatal ICU because of prema- RBC, red blood cellsturity, suspected sepsis, or respiratory distress syndrome. Twelve healthy adult volunteers served as a second group for the 86Rb Bmax, total specific uptake capacity uptake studies.Clinical studies. At the day of the study 1-ml heparinized blood samples were drawn from an existing intravenous indwellDespite continuous controversy over their clinical efficacy, ing catheter for 86Rb uptake studies. Electrolytes and creatinine digitalis glycosides are still one of the most commonly prescribed were determined in the serum and in 6-8 h urine collection. group of drugs (1). In infants and children, only a few controlled Creatinine clearance and fractional excretion of sodium and studies assessed the effect of digoxin in heart failure associated potassium were determined using standard methods. In each with congenital heart defects, and their results are inconclusive infant, potassium intake was calculated for the 2 days before the (2). Pharmacokinetic analysis has revealed that infants and chil-study and during the day of the study. dren need higher doses per kg of digoxin than adults to achieve 86Rb uptake studies. For the uptake assay of Rb in RBC the comparable serum concentrations due to faster clearance rates method described by Aronson et al. (1 1) was used with some (3). However, in some cases these differences are minimized by modifications. calculation of dose according to surface area.Erythrocytes were separated by centrifugation and washed The hypothesis that the immature organism is less s...

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The influence of age on Na-K-ATP-ase activity in erythrocytes in fetal and newborn guinea pigs

Cited by 9 publications

References 12 publications

Developmental changes in erythrocyte Na+,K+-ATPase subunit abundance and enzyme activity in neonates

Developmental changes in erythrocyte Na+,K+-ATPase subunit abundance and enzyme activity in neonates

The effect of digoxin on 86rubidium uptake by erythrocytes from mothers and babies.

Comparison of the Digitalis Receptor in Erythrocytes from Preterm Infants and Adults

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