The influence of amifostine administration prior to cyclophosphamide on in vitro maturation of mouse oocytes

Abstract

Purpose The protective effect of amifostine against cyclophosphamide (CP) was evaluated on mouse oocytes. Materials and methods Female mice were divided into four groups as follows: group1: cyclophosphamide (CP) (75 mg/kg, i.p) injection, group2: amifostine (250 mg/kg, i.p) injection, group3: amifostine (250 mg/kg, i.p) administered prior to CP (75 mg/kg, i.p) injection, Control group with injection of saline. About 21 days after injection, in vitro maturation (IVM) of oocytes was recorded. Furthermore the percentage of aneuploid oocytes was determined. Results In the CP group IVM rate was significantly decreased and aneploidy rate was significantly increased when compared to other groups (p<0.05). With the administration of Amifostine prior to CP injection IVM rate was increased and aneploidy rate was decreased. Discussion Depletion in IVM rate with administration of CP is due its adverse effects on oocyte quality. Amifostine administration prior to CP injection appears to modulate deleterious effects of CP on oocytes.

A universal method for generating knockout mice in multiple genetic backgrounds using zygote electroporation

“…The percentage of super-ovulated females, fertilisation rates, and embryo development rates in IVF and embryo transfer (Table 1) were not significantly different from those in previous reports (Byers et al, 2006). We did not examine the optimal conditions for performing super-ovulation treatments in each inbred strain, so there might be even better conditions for strains, such as BALB/c, which tend to have low fertilisation rates in a medium with low osmolarity or calcium concentration (Golkar-Narenji et al, 2012). The ovulation rate and number of oocytes obtained per female might be improved by hyper-ovulation treatment with anti-inhibin serum (Hasegawa et al, 2012) or in combination with oestrous cycle synchronisation (Hasegawa et al, 2022).…”