The global navigation satellite system has achieved great success in the civil and military fields and is an important resource for space-time information services. However, spoof interference has always been one of the main threats to the application security of satellite navigation receivers. In order to further improve the application security of satellite navigation receivers, this paper focuses on the application scenarios where narrowband and spoofing interference exist at the same time, studies the problem of spoofing interference detection under mixed interference conditions, then proposes a spoofing interference detection method based on the tracking loop identification curve. This method can effectively deal with the detection of spoofing interference under the conditions of narrowband interference and, at the same time, it can effectively detect the spoofing interference of gradual deviation. Simulation experiments verify the effectiveness of the spoofing interference detection method, based on the tracking loop discrimination curve. In typical jamming and spoofing scenarios, when the spoofing signal is about 7.5 m away from the real signal, the method used in this paper can achieve effective detection. The proposed detection method is of great significance for improving the anti-spoofing capability of satellite navigation receivers.