The objective of this experiment was to evaluate the effects of orally administered Megasphaera elsdenii NCIMB 41125 as a microbial supplement in steers abruptly transitioned from a receiving diet with 4% dietary starch [dry matter (DM) basis] to a growing diet with 38% dietary starch (DM basis). Steers [n = 192; initial shrunk body weight (SBW) = 309 kg ± 20.6 kg] were assigned to microbial supplement treatment in a randomized complete block design. Treatments were control (CON): no microbial supplement prior to diet transition, and (DFM): microbial supplement orally administered prior to diet transition [20 mL of microbial supplement (Lactipro NXT, Axiota Animal Health, Fort Collins, CO) containing 1 × 1010 colony forming units Megasphaera elsdenii NCIMB 41125]. Steers were source from a previously conduced 49 d feedlot receiving period experiment and abruptly transitioned from a receiving diet including soybean hulls and wheatlage containing 4% dietary starch (DM basis) to a growing diet including high-moisture ear corn, dry-rolled corn, and wheatlage containing 38% dietary starch (DM basis). Diets were switched on an equal DM intake basis to achieve the abrupt change and steers were fed the 38% starch diet for 49 d until experiment completion. Prior to experiment initiation, steers (n = 72; n = 3/pen) were fitted with wireless rumination tags to track daily activity and rumination time. No differences (P ≥ 0.20) were observed between treatments for final SBW, average daily gain, dry matter intake, feed efficiency, calculated net energy (NE) for maintenance and gain, or observed-to-expected ratio of NE for maintenance and gain. Additionally, no treatment × day or treatment differences (P ≥ 0.12) were observed for activity or rumination measures. Minutes ruminating and active both differed (P < 0.01) for the main effect of day. Compared to non-supplemented steers, oral administration of Megasphaera elsdenii NCIMB 41125 did not improve growth performance or efficiency of dietary net energy utilization in steers transitioned from a receiving diet containing 4% starch (DM basis) to a growing diet containing 38% starch (DM basis).