The influence of bio-conjugation on photoluminescence of CdSe/ZnS quantum dots

Torchynska, T. V.; Vorobiev, Yuri V.; Makhniy, V. P.; Horley, Paul

doi:10.1016/j.physb.2014.05.026

Cited by 7 publications

(6 citation statements)

References 44 publications

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“…Let us discuss other reasons for the PL energy shift at the bioconjugation of QDs to antibodies. These factors could be assigned to (i) the quantum-confined Stark effect stimulated by the charge of antibodies [43,44], (ii) the quantum-confined effect related to the shift of QD energy levels and stimulated by the change of electric potential at the QD surface [45], or (iii) decreasing the effective QD size in bioconjugated QDs [46]. The Stark effect is shifting and splitting of energy levels in atoms and molecules due to the presence of external electric field.…”

Section: Discussion Of the Cdse/zns Qd Bioconjugationmentioning

confidence: 99%

“…The other reason for the "blue" shift of QD energy levels at the quantum-confined condition, owing to decreasing the effective QD size in bioconjugated QDs, was considered by Torchynska et al [46]. When QD is bioconjugated, its energy balance changes very much.…”

Section: Discussion Of the Cdse/zns Qd Bioconjugationmentioning

confidence: 99%

“…We proposed to approximate it with a cylinder with a height c and a circular base of the diameter a. The Schrödinger equation for such system was solved using the mirror boundary conditions [46,47], providing the ground state energy relative to the material band gap [47]:…”

Section: Discussion Of the Cdse/zns Qd Bioconjugationmentioning

confidence: 99%

“…For these cases, the parameters of the effective cylinder are as follows: a = 1.6 R, c = 1.55 R (for 4 nm blocking radius) and a = 1.65 R, c = 1.6 R (for 3 nm blocking radius), where R = 3.2 nm is the radius of the CdSe core of the particle. Using these values, one can obtain the expressions for the ground state of conjugated nanoparticle with 4 nm blocking [46]:…”

Section: Discussion Of the Cdse/zns Qd Bioconjugationmentioning

confidence: 99%

“…As we mentioned above, these reasons can be related to (i) the emission of antibody molecules or PBS at UV excitation; (ii) the compressive strain applied to QDs at the bioconjugation [33]; (iii) the compound material degradation owing to core/shell intermixing [53] or oxidation [32] at the QD bioconjugation; (iv) the quantum-confined Stark effect [43,44], stimulated by electric charges of antibodies; (v) the dominated emission of excitons localized at the excited QD states; (vi) the change of energy band profile at the application of electric field of charged Abs; and (vi) the quantum-confined effect owing to the shift of QD energy levels stimulated by the change of potential barrier at the QD surface [45] or by decreasing the effective QD size in bioconjugated QDs [46]. To make the decision concerning the most probable physical reasons of the PL transformation in CdSeTe/ZnS QDs, let us analyze all of these factors in details.…”

Section: Discussion Of the Cdsete/zns Qd Bioconjugationmentioning

confidence: 99%

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Physical Reasons of Emission Varying in CdSe/ZnS and CdSeTe/ZnS Quantum Dots at Bioconjugation to Antibodies

Torchynska¹

2015

Quantum Dots - Theory and Applications

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Photoluminescence, its excitation power dependence, and Raman scattering spectra have been studied in CdSe/ZnS and CdSeTe/ZnS QDs for the nonconjugated states and after the QD conjugation to the anti-Interleukin-10, Human papilloma virus and Pseudo rabies virus antibodies. The QD bioconjugation to charged antibodies stimulates the "blue" energy shift of PL bands related to exciton emission in the CdSe or CdSeTe cores. The "blue" energy shift of PL spectrum in bioconjugated CdSe/ZnS QDs has been attributed to the electronic quantum confined effects stimulated by decreasing the effective QD size at its bioconjugation to charged antibodies. It was shown that the attachment of a charge deals with the antibody to the exterior shell of CdSe/ZnS QDs, leads to blocking away a fraction of core's volume. The energy band diagrams of CdSeTe/ZnS QDs in the nonconjugated and bioconjugated states have been designed, which permit to explain the types of optical transitions in QDs and their transformations at the QD bioconjugation. It is shown that the change of energy band profile and the "blue" shift of QD energy levels, owing to the change of potential barrier at the QD surface, are the dominant reasons of PL spectrum transformation in the double core CdSeTe/ZnS QDs conjugated to charged antibodies. Better understanding the QD bioconjugation to specific antibodies is expected to produce the major advances in biology and medicine and can be a powerful technique for early medical diagnostics.

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Section: Discussion Of the Cdse/zns Qd Bioconjugationmentioning

confidence: 99%

Section: Discussion Of the Cdse/zns Qd Bioconjugationmentioning

confidence: 99%

Section: Discussion Of the Cdse/zns Qd Bioconjugationmentioning

confidence: 99%

Section: Discussion Of the Cdse/zns Qd Bioconjugationmentioning

confidence: 99%

Section: Discussion Of the Cdsete/zns Qd Bioconjugationmentioning

confidence: 99%

See 3 more Smart Citations

Physical Reasons of Emission Varying in CdSe/ZnS and CdSeTe/ZnS Quantum Dots at Bioconjugation to Antibodies

Torchynska¹

2015

Quantum Dots - Theory and Applications

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Aspects of emission variation in CdSeTe/ZnS quantum dots conjugated to antibodies

Polupan

Torchynska

Gómez

et al. 2016

J Mater Sci: Mater Electron

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Biocompatible Water Soluble Polyacrylic Acid Coated CdSe/Cu Quantum Dot Conjugates for Biomolecule Detection

et al. 2017

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Biocompatible polyacrylic acid functionalized CdSe/Cu quantum dot conjugates were synthesized to be used for biomolecules detection. The study results demonstrate the conjugation of the 2.5-3 nm QD with gram negative bacteria with a low detection limit of 28 cfu/ml. The photoluminescence (PL) intensity was correlated to bacterial count, cellular proteins and exopolysaccharides in the tested samples. Confocal Scanning Laser Microscopy (CSLM) images showed significant QD uptake within the cells, both cytoplasm and DNA were the predominant targeted biomolecules, higher fluorescent uptake was shown in gram negative bacteria than that observed for gram positive bacteria. Moreover, PL showed that there was a distinction between live and dead cells as well as gram negative and gram positive cells. Cell viability was not affected even after 6 days (100% viability) rendering it a non-toxic QD. The method is simple and is performed in a single step within approximately 10 min as compared to multi-step protocols for classical microbial count or fluorescent dye staining. All the above results indicate that the CdSe/Cu-PAA QDs are suitable for biomolecule detection, bio-labeling and bioimaging applications.

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The influence of bio-conjugation on photoluminescence of CdSe/ZnS quantum dots

Cited by 7 publications

References 44 publications

Physical Reasons of Emission Varying in CdSe/ZnS and CdSeTe/ZnS Quantum Dots at Bioconjugation to Antibodies

Physical Reasons of Emission Varying in CdSe/ZnS and CdSeTe/ZnS Quantum Dots at Bioconjugation to Antibodies

Aspects of emission variation in CdSeTe/ZnS quantum dots conjugated to antibodies

Biocompatible Water Soluble Polyacrylic Acid Coated CdSe/Cu Quantum Dot Conjugates for Biomolecule Detection

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