The Influence of Ca²⁺ Concentration around Myofibrillar Mg²⁺-ATPase on the Speed and Pattern of Rigor Mortis in Fish Species or Cultured and Wild Fish

Abstract

The influence of Ca2+ concentration around myofibrillar Mg2+-ATPase on the speed and pattern of rigor mortis in fish species or cultured and wild fish was studied. The change of contraction in pillar form muscle with the lapse of storage time reflected very well the temporal change of gram of tension in muscle of cultured red sea bream. This result showed that the contractile percentage of muscle is very useful on the progress of rigor mortis. The progress of muscular contraction differed among fish species and between cultured and wild fish of the same species, and which were seen in four parameters of rigor mortis: the progressive speed, pattern, and the time and level of peak reached. These four parameters were closely related to one another.The ranking among fishes in myofibrillar Mg2+-ATPase activity was not consistent with the difference of speed of progression of muscular contraction. The progress of superprecipitation reaction of actomyosin in muscle of cultured red sea bream was changed remarkably in four parameters by a very small difference of Ca2+ concentration, coinciding very well with the mutual relation among the four parameters on the progress of muscular contraction. Therefore, it was considered that the difference of the progress of muscular contraction among fish species and between cultured and wild fish can be mainly caused by the difference of Ca2+ concentration around myofibrils.

Comparison of biochemical and physiological characteristics among white, pink, and red muscle fibers in carp (cultured)

“…The change of muscle length accompanying caffeine contraction was measured with the lapse of time, and was expressed as the contractile percentage of muscle. 14 …”

“…Previous reports have attributed the differences in rigor mortis progress of fish to several causes such as: the resolution speed of total ATP and the accumulation of lactic acid, 3–13 myofibrillar Mg 2+ ‐ATPase activity, 4,13–16 and Ca 2+ uptake by sarcoplasmic reticulum(SR). 4,16 Regarding the difference between cultured and wild red sea bream, we reported that the rigor mortis progress reached the maximum level earlier in cultured fish than in wild fish, and the muscular contractile percentage was also higher in cultured fish than in wild fish.…”

“… 4,16 Regarding the difference between cultured and wild red sea bream, we reported that the rigor mortis progress reached the maximum level earlier in cultured fish than in wild fish, and the muscular contractile percentage was also higher in cultured fish than in wild fish. 14 Furthermore, Iwamoto and Yamanaka 9 reported that the difference in rigor mortis progress between cultured and wild red sea bream was closely related to the discrepancies of ATP degradation and lactic acid accumulation in muscle, although the difference among fishes was not similar to these discrepancies of ATP and lactic acid. Tsuchimoto et al 14 considered that the difference among several fishes might be due to the discrepancy in the increasing speed of Ca 2+ concentration around the myofibrils.…”

“… 14 Furthermore, Iwamoto and Yamanaka 9 reported that the difference in rigor mortis progress between cultured and wild red sea bream was closely related to the discrepancies of ATP degradation and lactic acid accumulation in muscle, although the difference among fishes was not similar to these discrepancies of ATP and lactic acid. Tsuchimoto et al 14 considered that the difference among several fishes might be due to the discrepancy in the increasing speed of Ca 2+ concentration around the myofibrils.…”

Comparison of Ca2+ pump of sarcoplasmic reticulum in dorsal ordinary muscle between cultured red sea bream and Japanese flounder

“…The fish bodies of both fish sample species differed from specimens in our previous papers ( Table 1). 4,8,9 The 2‐year‐old fish had been cultivated at Toisu Bay, Nagasaki prefecture, and were purchased from a fish farmer in the summer with an ambient temperature of ~25°C. The fish were held in a laboratory water tank at an ambient temperature of 25°C for more than 2 months, and adequately fed the same moist pellets as the farm, before being used as experimental sample fish.…”