The influence of capillarization on satellite cell pool expansion and activation following exercise‐induced muscle damage in healthy young men

Nederveen, Joshua P.; Joanisse, Sophie; Snijders, Tim; Thomas, Aaron; Kumbhare, Dinesh; Parise, Gianni

doi:10.1113/jp275155

Cited by 60 publications

(94 citation statements)

References 56 publications

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“…These novel insights provided by Nederveen and colleagues () extend the group's previous work demonstrating that satellite cells are located significantly further away from capillaries in muscle of older (∼67 years) compared to younger (∼24 years) individuals (Nederveen et al . ).…”

supporting

confidence: 68%

“…A recent article published in The Journal of Physiology by Nederveen and colleagues () assessed the expansion and activation status of the satellite cell niche following exercise‐induced muscle fibre damage. Twenty‐nine previously untrained healthy young men undertook a bout of unilateral lower‐limb resistance exercise.…”

mentioning

confidence: 99%

“…Nonetheless, Nederveen and colleagues () are to be commended for their novel insights into the critical relationship between muscle capillarization and satellite cells during skeletal muscle repair following exercise. Characterizing the satellite cell's ‘orbit’ around capillaries provides one small step in understanding mechanisms regulating skeletal muscle repair, and one giant leap for exploration of the endomysium space.…”

mentioning

confidence: 99%

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supporting

confidence: 68%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Where do satellite cells orbit? An endomysium space odyssey

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“…; Nederveen et al . ). Human umbilical vein endothelial cells (HUVECs) have been shown to elicit mitogenic and myogenic effects on HMuSC in culture models, with secreted growth factors playing a major role (Christov et al .…”

Section: Introductionmentioning

confidence: 97%

Factors secreted from high glucose treated endothelial cells impair expansion and differentiation of human skeletal muscle satellite cells

Kargl¹,

Nie²,

Evans³

et al. 2019

The Journal of Physiology

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Key points Cellular communication occurs between endothelial cells and skeletal muscle satellite cells and is mitogenic for both cell types under normal conditions. Skeletal muscle atrophy and endothelial cell dysfunction occur in tandem in cardiovascular disease, type II diabetes and ageing. The present study investigated how induction of endothelial cell dysfunction via high glucose treatment impacts growth and differentiation of human skeletal muscle satellite cells in vitro. Secreted factors from high glucose treated endothelial cells impaired satellite cell expansion and differentiation via decreased proliferation and dysregulation of p38 mitogen‐activated protein kinase in satellite cells committed to myogenesis. These findings highlight a novel potential role for endothelial cells in the development and pathology of skeletal muscle atrophy, which is common in patients with endothelial dysfunction related pathologies. Abstract Cross‐talk between endothelial cells (ECs) and skeletal muscle satellite cells (MuSC) has been identified as an important regulator of cellular functions in both cell types. In healthy conditions, EC secreted factors promote MuSC growth and differentiation. Endothelial and satellite cell dysfunction occur in tandem in many disease states; however, no data exist examining the impact of dysfunctional EC signalling on satellite cells. Therefore, the present study aimed to evaluate the effect that factors secreted from high glucose (HG) treated ECs have on the growth and differentiation of human satellite cells (HMuSC) using a conditioned medium (CM) cell culture model. Satellite cells were isolated from human skeletal muscle and grown in CM from normal or HG treated human umbilical vein ECs (HUVECs). Satellite cells grown in CM from HG treated HUVECs reduced growth (25%), differentiation (25%) and myonuclear fusion (35%). These responses were associated with increased superoxide (50%) and inflammatory cytokines (25–50%) in HG treated HUVECs and HG‐CM. Decreased expansion of HG‐CM treated HMuSCs was driven by a decrease in proliferation. Impaired gene expression and protein content of myogenic differentiation factors were preceded by decreased phosphorylation of p38 mitogen‐activated protein kinase in HMuSC treated with CM from HG treated HUVECs. The results obtained in the present study are the first to show that factors secreted from HG treated ECs cause impairments in human muscle satellite cell growth and differentiation in vitro, highlighting endothelial cell health and secretion as a potential target for treating vascular disease‐associated skeletal muscle dysfunction.

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“…In line, the acute muscle satellite cell activation response during post‐exercise recovery has been reported to depend on muscle fibre capillarization in young men (Nederveen et al . , ). As muscle fibre capillarization declines with advancing age, it has been hypothesized that the impaired acute satellite cell response during post‐exercise recovery may, in part, be due to age‐related reduction in muscle fibre capillarization in older adults (Joanisse et al .…”

Section: Introductionmentioning

confidence: 99%

Prolonged exercise training improves the acute type II muscle fibre satellite cell response in healthy older men

Snijders

Nederveen

Bell

et al. 2018

The Journal of Physiology

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Key points Skeletal muscle stem cells, termed satellite cells, play a crucial role in repair and remodelling of muscle in response to exercise An age‐related decline in satellite cell number and/or function has been hypothesized to be a key factor in the development of sarcopenia and/or the blunted muscle fibre adaptive response to prolonged exercise training in older persons We report that performing prolonged exercise training improves the acute type II muscle fibre satellite cell response following a single bout of resistance exercise in older men. The observed improvement in muscle satellite function is associated with an increase in muscle fibre capillarization following exercise training suggesting a possible functional link between capillarization and satellite cell function. Abstract Age‐related type II muscle fibre atrophy is accompanied by a fibre type‐specific decline in satellite cell number and function. Exercise training restores satellite cell quantity in older adults; however, whether it can restore the impaired satellite cell response to exercise in older adults remains unknown. Therefore we assessed the acute satellite cell response to a single exercise session before and after prolonged exercise training in older men. Fourteen older men (74 ± 8 years) participated in a 12‐week exercise training programme (resistance exercise performed twice per week, high intensity interval training once per week). Before and after training, percutaneous biopsies from the vastus lateralis muscle were taken prior to and following 24 and 48 h of post‐exercise recovery. Muscle fibre characteristics were evaluated by immunohistochemistry and mRNA expression by RT‐PCR. Whereas no changes were observed in type II muscle fibres, type I muscle fibre satellite cell content increased significantly at 24 and 48 h after a single bout of resistance exercise before the exercise training programme (P < 0.01). Following the exercise training programme, both type I and type II muscle fibre satellite cell content increased significantly at 24 and 48 h after a single bout of resistance exercise (P < 0.05). The greater acute increase in type II muscle fibre satellite cell content at 24 h post‐exercise recovery after training was correlated with an increase in type II muscle fibre capillarization (r = 0.671, P = 0.012). We show that the acute muscle satellite cell response following exercise can be improved by prolonged exercise training in older men.

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The influence of capillarization on satellite cell pool expansion and activation following exercise‐induced muscle damage in healthy young men

Cited by 60 publications

References 56 publications

Where do satellite cells orbit? An endomysium space odyssey

Where do satellite cells orbit? An endomysium space odyssey

Factors secreted from high glucose treated endothelial cells impair expansion and differentiation of human skeletal muscle satellite cells

Prolonged exercise training improves the acute type II muscle fibre satellite cell response in healthy older men

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