The influence of cell elastic modulus on inertial positions in Poiseuille microflows

Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions

“…Before experimentation, all cell types were counted using a LUNA Automated Cell Counter and resuspended at a concentration of approximately 750 cells/µ L in serum-free Dulbecco's Modified Eagles Medium (DMEM, Sigma-Aldrich Inc, Dublin, Ireland) and 20% Percoll (Sigma-Aldrich Inc, Dublin, Ireland). This has previously been found to be the optimal percentage of Percoll to prevent cell settling and adhesion 43 and has also previously been shown to have no effect on cell viability 44 . The lower limit of the cell concentration used was dictated by the number of cells required to produce an accurate result following sample acquisition from the cell suspension reservoir, while the upper limit was dictated by the need to reduce the effects due to cellcell interactions.…”

“…Further study is therefore required in order to investigate if other factors can be used to more accurately predict cell viability in microchannels such as ∇τ p or the shear gradient across the channel width, as has been previously suggested 10,11 . Previous studies have also shown that the location occupied by suspended cells in a channel can be dictated by the deformation properties of that cell, a property which is unique to each cell line 43 . It remains to be seen whether this location ∇τ p or the shear gradient acting on the cell and therefore its viability.…”

Isosceles Trapezoidal Spiral Microchannel for Separation of Large particles and Giant Cancer cells

“…The decrease is mainly due to the difference in the deformability between cells (E = 0.1-15 kPa) [49][50][51] and PS particles (E = 2-8 GPa) [52]. Cells with low E experience an additional deformable induced lift by the ow [40,53]. This F L coupled with F D is known to make the focusing position of the cells shift away from the inner wall of the channel compared to the focusing point of particles with high E.…”

Hemodynamics and Hemorheology

“…On microscopic scale, the structuring of the suspension responsible for these phenomena is governed by two antagonistic processes which are, firstly, a migration of cells away from the walls and toward the center of the channel, and secondly, a shear-induced diffusion and interactions between red blood cells. These processes, which are common to all suspensions of deformable objects, have formed the subject of works permitting scaling laws to be proposed (Podgorski et al 2011;Grandchamp et al 2013;Losserand et al 2019), and are strongly influenced by the mechanical properties of circulating cells, which can make them tools of interest for diagnosis, indirect characterization of these properties or cell sorting (Geislinger and Franke 2013;Connolly et al 2021). In a polydisperse system such as blood, this sensitivity to cell properties also leads to margination and segregation phenomena, where the most rigid and smallest cells tend on average to be closer to the walls (white blood cells, platelets and, potentially, more rigid pathological red blood cells).…”