The influence of clinical and demographic risk factors on the establishment of head and neck squamous cell carcinoma cell lines

Abstract

The purpose of this study was to generate stable cell cultures from head and neck squamous cell carcinomas (HNSCC), and retrospectively analyze the factors associated with successful cell line establishment. Fifty-two HNSCC cell lines were isolated from a series of 199 tumors collected between 1992 and 1997 at the University of Pittsburgh Medical Center. Cell lines were characterized at the molecular and cellular level to determine the features associated with cell line formation. Successful cell line formation was dependent on multiple factors, including gene amplification involving chromosomal band 11q13, local and/or regional involvement of lymph nodes, and alcohol usage. The establishment of HNSCC cell lines enriches the resources available for cancer research. Our findings indicate that generation of stable cell lines from HNSCC is biased towards tumors with a poor prognosis. Our 52 stable lines comprise one of the largest series of HNSCC cell lines in the literature, with complete demographic, histopathologic, clinical, and survival data.

Genome-wide analysis of HPV integration in human cancers reveals recurrent, focal genomic instability

“…UM-SCC-47 (Lansford et al 1999) and UM-SCC-104 (Tang et al 2012) were kindly provided by Thomas Carey, University of Michigan; UPCI:SCC090 (White et al 2007) were from Susanne M. Gollin, University of Pittsburgh; UD-SCC-2 (Ballo et al 1999) were from Henning Bier, University of Dusseldorf; and HMS001 cells were from James Rocco, Harvard Medical School. Please see Table 1 and Supplemental Table 1. CAL 27, D562, SCC-25, SiHa, and CaSki cells were cultured as recommended by ATCC.…”

Evaluation of human papillomavirus testing for squamous cell carcinoma of the tonsil in clinical practice

“…HPV DNA was analysed by PCR using the improved general primer set GP5+/6+ which amplifies part of the HPV L1 gene encoding the HPV major capsid protein, detecting low-risk genotypes -6, -11, -40, -42, -43 and -44 and high-risk genotypes -16, -18, -31, -33, -35, -39, -45, -51, -52, -56, -58, -59, -66 and -68 as previously described 10. DNA from CaSki cells was used as a positive control, and DNA from the UPCI:SCC089 oropharyngeal squamous cell carcinoma cell line13 and omission of template DNA were used as negative controls.…”

Chemosensitization of head and neck cancer cells by PUMA

“…Cell Culture and DrugTreatment T h e c e l l l i n e s U P C I : S C C 0 0 3 , U P C I : S C C 1 0 4 , UPCI:SCC105, UPCI:SCC116 (28,29), 1483 (30), UPCI-15B (15B; ref. 31), UM-SCC-22A, UM-SCC-22B, JHU-012 (32), and two early-passage normal human keratinocyte primary cultures, HN1771 and HN1871 (28), used in the study were from the University of Pittsburgh Cancer Institute (UPCI) Head and Neck cancer program.…”