The influence of combined Fpg- and MutY-deficiency on the spontaneous and γ-radiation-induced mutation spectrum in the lacZα gene of M13mp10

Kuipers, Gitta K.; Slotman, Ben J.; Poldervaart, Hester A; Reitsma-Wijker, Carola A; Lafleur, M.V.M.

doi:10.1016/s0921-8777(00)00050-1

Cited by 12 publications

(7 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The overwhelming abundance of A·T to G·C transitions in the pre-existing spectra does not permit an easy correlation of these mutations with oxidative DNA damage. However, it cannot be ruled out, because, DNA glycosylases present in the cell remove selected oxidized bases from DNA, and can greatly alter the spectrum of mutations [70][71][72][73]. This question can be addressed by analyzing the profiles of oxidized base lesions and DNA glycosylases in the mammary tissue.…”

Section: Discussionmentioning

confidence: 99%

Induction of A·T to G·C mutations by erroneous repair of depurinated DNA following estrogen treatment of the mammary gland of ACI rats

Mailander

Meza

Higginbotham

et al. 2006

The Journal of Steroid Biochemistry and Molecular Biology

117

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Induction of A·T to G·C mutations by erroneous repair of depurinated DNA following estrogen treatment of the mammary gland of ACI rats

Mailander

Meza

Higginbotham

et al. 2006

The Journal of Steroid Biochemistry and Molecular Biology

117

View full text Add to dashboard Cite

“…8-OxoG is believed to represent a major source of ROS-induced mutagenesis in all aerobic cells because of their persistent generation, relative abundance, and potent mutagenicity. An Ogg1 deficiency in yeast, as well as an Fpg deficiency in bacteria, results in a mutator phenotype (31,78). Similarly, Ogg1-deficient mice show an abnormal accumulation of 8-oxoG in their genome and exhibit a significantly higher mutation rate (79).…”

Section: Discussionmentioning

confidence: 99%

Cadmium Down-regulates Human OGG1 through Suppression of Sp1 Activity

Youn

Kim

Lee

et al. 2005

Journal of Biological Chemistry

View full text Add to dashboard Cite

Cadmium is a well known human and animal carcinogen and is a ubiquitous contaminant in the environment. Although the carcinogenic mechanism of cadmium is a multifactorial process, oxidative DNA damage is believed to be of prime importance. In particular, cadmium suppresses the capacity of cells to repair oxidative DNA damage. In this study, cadmium treatment led to a significant increase in ␥-ray-induced 8-oxoguanine (8-oxoG) formation. Western blotting and semiquantitative reverse transcription-PCR revealed that cadmium treatment caused a decrease in the expression level of human OGG1 (8-oxoguanine-DNA glycosylase-1; hOGG1) in human fibroblast GM00637 and HeLa S3 cells. In addition, the cadmium-mediated decrease in hOGG1 transcription was the result of decreased binding of the transcription factor Sp1 to the hOGG1 promoter. Finally, we show that an increase in the functional hOGG1 expression level could inhibit the cadmium-mediated increase in ␥-ray-induced 8-oxoG accumulation as well as in ␥-radiation-induced mutation frequency at the HPRT (hypoxanthine-guanine phosphoribosyltransferase) gene locus. These results suggest that cadmium attenuates removal of ␥-ray-induced 8-oxoG adducts, which in turn increases the mutation frequency, and that this effect might, at least in part, result from suppression of hOGG1 transcription via inactivation of Sp1 as a result of cadmium treatment.

show abstract

“…This is one of the main types of mutation induced by some oxidative agents [Retél et al, 1993;Akasaka and Yamamoto, 1995;Agnez-Lima et al, 1999]. Recently, MutY-glycosylase has also been implicated in mismatch repair involving oxidized and normal guanines [Kuipers et al, 2000;Agnez-Lima et al, 2001], which can lead to G3 C transversions. This is another important type of mutation induced by oxidative agents [Retél et al, 1993;Akasaka and Yamamoto, 1995;Agnez-Lima et al, 1999.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of mutagenic activity in an extract of pepper tree stem bark (Schinus terebinthifolius Raddi)

Carvalho

Barca

Agnez-Lima

et al. 2003

Environ and Mol Mutagen

View full text Add to dashboard Cite

An extract (decoction) from pepper tree stem bark (Schinus terebinthifolius Raddi) is widely used in Brazil as a topical antiinflammatory agent and to cicatrize wounds. The extract contains catechin, tannins, terpenes, flavonoids, and saponins; of these components, both mutagenic potential and antioxidant properties have been ascribed to flavonoids. The mutagenicity of some flavonoids is believed to be associated with the formation of reactive oxygen species and seems to depend on the number and position of hydroxyl groups. In the present study, we evaluated an extract of S. terebinthifolius in a series of cell-free and bacterial assays in order to determine its genotoxic potential. The extract was negative in a cell-free plasmid DNA test, indicating that it did not directly break DNA. Positive results, however, were obtained in the SOS chromotest, in a forward mutagenesis assay employing CC104 and CC104mutMmutY strains of Escherichia coli, and in the Salmonella reversion assay, using strains TA97, TA98, TA100, and TA102. All the bacterial tests were performed without exogenous metabolic activation due to the topical use of this preparation. The results indicate that pepper tree stem bark extract produces DNA damage and mutation in bacteria, and that oxidative damage may be responsible for the genotoxicity.

show abstract

The influence of combined Fpg- and MutY-deficiency on the spontaneous and γ-radiation-induced mutation spectrum in the lacZα gene of M13mp10

Cited by 12 publications

References 25 publications

Induction of A·T to G·C mutations by erroneous repair of depurinated DNA following estrogen treatment of the mammary gland of ACI rats

Induction of A·T to G·C mutations by erroneous repair of depurinated DNA following estrogen treatment of the mammary gland of ACI rats

Cadmium Down-regulates Human OGG1 through Suppression of Sp1 Activity

Evaluation of mutagenic activity in an extract of pepper tree stem bark (Schinus terebinthifolius Raddi)

Contact Info

Product

Resources

About