The influence of conjugated estrogens in radioimmunoassays using different antibodies against estradiol-17β

The effect of passive immunization against oestrogens on the onset of parturition in the ewe

“…Oestrogen sulphates may have oestrogenic effects on the uterus (Pierrepoint et al, 1971), but in the ovariectomized ewe oestradiol benzoate appears to be 20 times more effective in stimulating uterine motility than oestrone sulphate (unpublished results of J. Prud'Homme, cited by Bosc, Delouis & Terqui, 1977). The present experiment investigated only the involvement of unconjugated oestrogens in the maternal circulation and the antisera used would probably not have bound any oestrogen conjugates (Boilert, Edqvist, Johannson, Lindberg & Martinsson, 1973).…”

Plasma concentrations of 15-keto-13,14-dihydro-PGF-2 , oestrone sulphate, oestradiol-17 and progesterone in pregnant guinea-pigs treated with polychlorinated biphenyls

“…The oestradiol antiserum was raised in sheep against oestradiol-17ß-6-(0-carboxymethyl)-oxime-bovine serum albumin (Boilert et al, 1973). To each tube was added 100 µ diluted antiserum (1: 600 000) and 100 µ (01 pmol) [2,4,6,7-3H]oestradiol-17ß (sp.…”

Flushing of Mink

“…which had an 11 % cross-reaction with estrone (Boilert et al, 1973;Lindberg et al, 1974). Plasma (100 111 aliquots) was extracted once with 10-volumes of diethyl ether (Mallinckrodt) with an extration recovery of about 90%.…”