The influence of contact guidance on chemotaxis of human neutrophil leukocytes

Wilkinson, P. C.; Lackie, J. M.

doi:10.1016/0014-4827(83)90004-6

Cited by 53 publications

(21 citation statements)

References 8 publications

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“…These reconstruction data support previous work that suggests physical contact guidance mechanisms to direct leukocyte migration in scaffolds independently of proteolytic ECM breakdown. 6,44,45 In regions of narrow space, depending on the pore size, 2 different mechanisms sustain nonproteolytic movement. First, squeezing through narrow gaps by adapting the cell shape and the formation of a constriction ring, 38 followed by cytoplasmic streaming through this immobile external confinement zone.…”

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confidence: 99%

“…on May 11, 2018. by guest www.bloodjournal.org From Amoeboid shape change: a nonproteolytic, biophysical migration mechanism bypassing matrix barriers Previous dynamic reconstruction on nonlabeled neutrophils, T cells, and dendritic cells show leukocyte movement in collagen or amnionic membrane as a contact guidance-dependent process that appears to follow pre-existing matrix scaffolds. 4,6,[44][45][46] We have used improved dynamic real-time reconstruction of fluorescently labeled cells together with high-resolution imaging of the 3D matrix architecture. The typical random paths and turns developed by T cells in 3D collagen lattices result from stringent alignment of the cell body in parallel to fibrils and fiber bundles that border random gaps and trails pre-existing in the lattice.…”

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confidence: 99%

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Amoeboid shape change and contact guidance: T-lymphocyte crawling through fibrillar collagen is independent of matrix remodeling by MMPs and other proteases

Wolf

Mueller

Borgmann

et al. 2003

Blood

391

314

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The passage of leukocytes through basement membranes involves proteolytic degradation of extracellular matrix (ECM) components executed by focalized proteolysis. We have investigated whether the migration of leukocytes through 3-dimensional collagenous tissue scaffolds requires similar ECM breakdown. Human T blasts and SupT1 lymphoma cells expressed mRNA of MMP-9, MT1-MMP, MT4-MMP, cathepsin L, uPA, and uPAR as well as ADAM-9, -10, -11, -15, and -17. Upon long-term migration within 3-dimensional collagen matrices, however, no in situ collagenolysis was obtained by sensitive fluorescein isothiocyanate-collagen fragmentation analysis and confocal fluorescence/backscatter microscopy. Consistent with nonproteolytic migration, T-cell crawling and path generation were not impaired by protease inhibitor cocktail targeting MMPs, serine proteases, cysteine proteases, and cathepsins. Dynamic imaging of cell-ECM interactions showed T-cell migration as an amoebalike process driven by adaptive morphology, crawling along collagen fibrils (contact guidance) and squeezing through pre-existing matrix gaps by vigorous shape change. The concept of nonproteolytic amoeboid migration was confirmed for multicomponent collagen lattices containing hyaluronan and chondroitin sulfate and for other migrating leukocytes including CD8 ؉ T blasts, monocyte-derived dendritic cells, and U937 monocytic cells. Together, amoeboid shape change and contact guidance provide constitutive protease-independent mechanisms for leukocyte trafficking through interstitial tissues that are insensitive toward pharmacologic protease inhibitors. IntroductionThe migration and recirculation of T lymphocytes through the tissues is a multistep process that requires cell adhesion coupled with cellular strategies to overcome physical tissue barriers. 1 The principles of T-cell migration follow the paradigm of "amoeboid" movement established for the single cell state of the lower eucaryote Dictyostelium discoideum. 2,3 Amoeboid movement is a fast lowaffinity migration type driven by a roundish yet flexible cell morphology, dynamic and polarized pseudopod protrusions and retractions, which are independent of focal contact formation and stress fibers. 2 Similar to Dictyostelium, migrating T lymphocytes show an elliptoid polarized shape with a smooth yet dynamically ruffling leading edge, and an additional trailing uropod. 4 This shape generates fast (5-25 m/min) low-affinity gliding across surfaces and through 3-dimensional (3D) collagenous scaffolds 4 driven by a diffuse cortical actin cytoskeleton along the inner leaflet of the plasma membrane devoid of focal contacts at substrate interactions and stress fibers. 5,6 Amoeboid T-cell migration within extracellular matrix (ECM) occurs independently of ␤1 integrin-mediated adhesion both in vitro 6 and in vivo, 7 suggesting differences of the molecular basis between the movement of lymphocytes and other mobile cells, such as fibroblasts and tumor cells. 8,9 After transendothelial migration, T cells and other leukocytes ent...

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confidence: 99%

mentioning

confidence: 99%

Amoeboid shape change and contact guidance: T-lymphocyte crawling through fibrillar collagen is independent of matrix remodeling by MMPs and other proteases

Wolf

Mueller

Borgmann

et al. 2003

Blood

391

314

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“…Interest in illuminating these phenomena led to the development of first-generation biomolecule gradient platforms including chambers designed by Boyden, 1 Zigmond, 2 and Dunn, 3 as well as 3D gel matrices for diffusible molecules. 4,5 Limitations in spatiotemporal control and a lack of quantitative characterization of these systems have motivated the development of flow-based microfluidic devices that exploit the unique mass-transfer phenomena present at the microscale to more closely reproduce in vivo cellular environments. [6][7][8][9][10] While these microfluidic systems provide powerful methods for controlling a gradient's shape and function, they suffer from a number of drawbacks: ͑1͒ Gradient flow tends to remove cell-secreted molecules essential for cell-cell communication, ͑2͒ flow generates potentially damaging shear forces on cellular membranes, and ͑3͒ enclosed microfluidic channels are required for many flow-based devices, limiting access to cultured cells.…”

Section: A Microfluidic Platform For Generation Of Sharp Gradients Inmentioning

confidence: 99%

A microfluidic platform for generation of sharp gradients in open-access culture

2010

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Control of the 3D microenvironment for cultured cells is essential for understanding the complex relationships that biomolecular concentration gradients have on cellular growth, regeneration, and differentiation. This paper reports a microfluidic device for delivering gradients of soluble molecules to cells in an open reservoir without exposing the cells to flow. The cells are cultured on a polyester membrane that shields them from the flow that delivers the gradient. A novel "lid" design is implemented which prevents leakage from around the membrane without requiring sealing agents or adhesives. Once layers are molded, device fabrication can be performed within minutes while at room temperature. Surface gradients were characterized with epifluorescence microscopy; image analysis verified that sharp gradients (∼33 μm wide) can be reproducibly generated. We show that heterogeneous laminar flow patterns of Orange and Green Cell Tracker (CT) applied beneath the membrane can be localized to cells cultured on the other side; concentration profile scans show the extent of CT diffusion parallel to the membrane's surface to be 10-20 μm. Our device is ideal for conventional cell culture because the cell culture surface is readily accessible to physical manipulation (e.g., micropipette access), the cell culture medium is in direct contact with the incubator atmosphere (i.e., no special protocols for ensuring proper equilibration of gas concentrations are required), and the cells are not subjected to flow-induced shear forces, which are advantageous attributes not commonly found in closed-channel microfluidic designs.

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“…Such a peak of leukocyte chemotaxis occurring at a much lower concentration than that causes a prominent respiratory burst [7], Therefore. CL in neutrophils was observed to increase dose-dependently.…”

Section: Discussionmentioning

confidence: 99%

“…A premature activation of oxidative metabolism in neutrophils was thought to start only after they have moved up a certain gradient of chemotactic fac tors [7], However, we have offered evidence that CL was measurable in neutrophils even during chemotactic mi gration [8], Furthermore, in this report we suggest that the chemoattractant itself or to other chemical mediators chemotactic factors for neutrophils may be classified into two types, that is, oxidative metabolism-related and nonrelated and that the generation of superoxide in neutro phils seems to play an important role in the initial stage of chemotactic locomotion to some chemotactic factors.…”

Section: Introductionmentioning

confidence: 99%

Lucigenin-Induced Chemiluminescence in Human Neutrophils in the Process of Chemotactic Migration Measured in a Modified Boyden Chamber

Kato¹,

Terada²,

Iizawa³

et al. 1989

Dermatology

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To the question of whether an oxidative metabolism of neutrophils occurs in the process of chemotactic migration, we have already demonstrated that formyl-peptide and zymosan-activated serum effectively induced lucigenin-dependent chemiluminescence (CL) in a specially devised Boyden chamber. In the present study we confirmed this and, furthermore, demonstrated that superoxide dismutase partially suppressed the neutrophil chemotaxis to formyl-peptide and concanavalin A, suggesting the participation of superoxide in a chemotactically migrating mechanism. However, monocyte-derived chemotactic factor did not cause any light emission, irrespective of its chemotactic activity. Based on these results, neutrophil chemotactic factors seem to be divided into two types, that is, oxidative metabolism-related and nonrelated. To the former group of chemotactic factors a premature activation of oxidative metabolism in neutrophils may start even at the initial stage of chemotaxis, and these primed cells may respond with a respiratory burst to higher concentrations of the chemoattractant itself or to other chemical mediators present at the site of inflammation.

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The influence of contact guidance on chemotaxis of human neutrophil leukocytes

Cited by 53 publications

References 8 publications

Amoeboid shape change and contact guidance: T-lymphocyte crawling through fibrillar collagen is independent of matrix remodeling by MMPs and other proteases

Amoeboid shape change and contact guidance: T-lymphocyte crawling through fibrillar collagen is independent of matrix remodeling by MMPs and other proteases

A microfluidic platform for generation of sharp gradients in open-access culture

Lucigenin-Induced Chemiluminescence in Human Neutrophils in the Process of Chemotactic Migration Measured in a Modified Boyden Chamber

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