The influence of copper levels on <i>in vitro</i> ruminal fermentation, bacterial growth and methane production

Hernández-Sánchez, David; Cervantes-Gómez, Daniel; Ramírez-Bribiesca, J. Efrén; Cobos-Peralta, Mario A.; Pinto-Ruíz, René; Astigarraga, Laura; Gere, José Ignacio

doi:10.1002/jsfa.9274

Cited by 17 publications

(8 citation statements)

References 27 publications

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“…However, digestibility of NDF and ADF, ruminal microbial population and enzyme activity were similar for MCCS and HCCS addition, indicating that higher level of Cu in the rumen might not be necessary. Similarly, Hernández-Sánchez et al (30) found that ruminal total bacteria population and VFA concentration were unchanged with increasing addition of Cu (as CS) from 20, 40 to 60 mg/kg DM and were decreased by addition of 80 and 100 mg/kg DM of Cu as CS in vitro. The decrease in ruminal propionate percentage was consistent with the lower activity of α-amylase and populations of P. ruminicola and Rb.…”

Section: Discussionmentioning

confidence: 86%

Effects of copper sulphate and coated copper sulphate addition on lactation performance, nutrient digestibility, ruminal fermentation and blood metabolites in dairy cows

et al. 2020

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Coated copper sulfate (CCS) could be used as copper supplement in cows. To investigate the influences of copper sulfate (CS) and CCS on milk performance, nutrient digestion and rumen fermentation, fifty Holstein dairy cows were arranged in a randomized block design to five groups: control, CS addition (7.5 mg Cu/kg dry matter [DM] from CS) or CCS addition (5, 7.5 and 10 mg Cu/kg DM from CCS, respectively). When comparing copper source at equal inclusion rates (7.5 mg/kg DM), cows receiving CCS addition had higher yields of fat corrected milk, milk fat and protein, digestibility of DM, organic matter (OM) and neutral detergent fibre (NDF), ruminal total volatile fatty acid (VFA) concentration, activities of carboxymethyl-cellulase, cellobiase, pectinase and α-amylase, populations of Ruminococcus (R.) albus, R. flavefaciens and Fibrobacter succinogenes and liver copper content than cows receiving CS addition. Increasing CCS addition, DM intake was unchanged, yields of milk, milk fat and protein, feed efficiency, digestibility of DM, OM, NDF and acid detergent acid fibre, ruminal total VFA concentration, acetate to propionate ratio, activity of cellulolytic enzyme and populations of total bacteria, protozoa and dominant cellulolytic bacteria and concentrations of copper in serum and liver increased linearly, but ruminal propionate percentage, ammonia-N concentration, α-amylase activity and populations of Prevotella ruminicola and Ruminobacter amylophilus decreased linearly. The results indicated that supplement of CS could be substituted with CCS, and addition of CCS improved milk performance and nutrient digestion in dairy cows.

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Section: Discussionmentioning

confidence: 86%

Effects of copper sulphate and coated copper sulphate addition on lactation performance, nutrient digestibility, ruminal fermentation and blood metabolites in dairy cows

et al. 2020

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“…However, when the concentration of the mineral reaches this threshold, bacterial proteolysis occurs [37]. Hernández-Sánchez et al [38] reported that raising the amount of copper sulfate from 20, 40 to 60 mg/kg DM had no effect on VFA concentration on in vitro trials. On the other hand, increasing the concentration of copper sulfate to 80 and 100 mg/kg DM decreased the total amount of rumen bacteria and, therefore, the concentration level of VFA.…”

Section: Discussionmentioning

confidence: 99%

Effects of Copper Sulfate and Encapsulated Copper Addition on In Vitro Rumen Fermentation and Methane Production

Wilk

Pecka-Kiełb

Pastuszak³

et al. 2022

Agriculture

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Copper is a microelement crucial for the proper functioning of animals’ metabolic processes. The function of copper in rumen fermentation processes and methanogenesis is not well analyzed. The aim of the study was to evaluate the different types of copper supplement, their rumen decomposition and effect on in vitro ruminal fermentation as well as methanogenesis. Two different copper additives were used in the experiment: CS—copper sulfate (CuSO4 × 5 H2O)—and EC—encapsulated copper (tribasic copper chloride and copper sulfate enclosed within a polysaccharide polymer coating). A total mixed ration without copper additive was used as a control (C). In vitro rumen fermentation was conducted, and fermentation profile, gas production and methanogenesis were evaluated. After 24 h of fermentation, the amount of copper in the rumen fluid was significantly higher in the CS group. EC was protected against rumen degradation to a greater extent. The type of used copper supplement affects rumen fermentation. However, the effect on methanogenesis is ambiguous. CS supplement increases rumen gas production but does not affect methanogenesis. The obtained results suggest that the EC supplement may reduce the risk of low-fat milk and may improve the economic indicators of milk production. An in vivo experiment is necessary to compare the obtained in vitro results with animal productivity.

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“…It has been previously reported that different trace minerals have toxic effects on methanogens. Hernandez-S´anchez ´ et al [25] reported that Cu decreases CH4 production because it is toxic to some rumen methanogens. Liu et al [26] further reported that mineral supplementation reduces enteric methane emissions by altering the rumen microbiome.…”

Section: A C C E T E Dmentioning

confidence: 99%

Influence of dietary organic trace minerals on enteric methane emissions and rumen microbiota of heat-stressed dairy steers

Son¹,

Islam²,

Kim³

et al. 2023

J Anim Sci Technol

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The influence of copper levels on in vitro ruminal fermentation, bacterial growth and methane production

Abstract: Overall, the addition of increasing levels of Cu to 40 µg Cu/g DM did not have an adverse impact on ruminal bacteria growth and decreased CH production, without affecting the ruminal kinetics. © 2018 Society of Chemical Industry.

Cited by 17 publications

References 27 publications

Effects of copper sulphate and coated copper sulphate addition on lactation performance, nutrient digestibility, ruminal fermentation and blood metabolites in dairy cows

Effects of copper sulphate and coated copper sulphate addition on lactation performance, nutrient digestibility, ruminal fermentation and blood metabolites in dairy cows

Effects of Copper Sulfate and Encapsulated Copper Addition on In Vitro Rumen Fermentation and Methane Production

Influence of dietary organic trace minerals on enteric methane emissions and rumen microbiota of heat-stressed dairy steers

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