The influence of dietary conjugated linoleic acid isomers and Se on the fatty acid profile in rat blood plasma and selected tissues

Abstract

The objective of this study was to determine the conjugated linoleic acid (CLA) isomer composition of blood plasma and other organ tissues of Wistar rats fed a diet enriched with 1 or 2% CLA isomer(s) and 2 ppm Se as sodium selenate for 4 weeks. The dietary supplement of CLA isomers significantly elevated the concentration of CLA isomers and other fatty acids containing conjugated double bonds in plasma, spleen, pancreas, heart, and kidney tissues. cis9,trans11CLA was preferentially accumulated in plasma and organ tissues of rats fed the CLA isomer mixture with or without Se, while the percentage contribution of trans10,cis12CLA in the tissues of organs tended to be lower than its percentage in the administered CLA isomer mixture. Dietary CLA isomer(s) increased the Ca content of whole blood, while the diet with the CLA isomer(s) and Se increased the concentration of Mg and Se in spleen tissue. The experimental diets did not produce any substantial changes in the Fe or Zn concentrations in blood or spleen tissue. The diet enriched in trans10,cis12CLA and Se significantly increased spleen, pancreas and heart weights. Regardless of the presence of Se, the diet enriched in CLA isomer(s) stimulated the accumulation of polyunsaturated fatty acids in blood plasma, and in pancreas, heart, and kidney tissues. The diets enriched in the CLA isomer(s) usually resulted in a decrease of the monounsaturated fatty acid concentration and Δ9-desaturase capacity in plasma, spleen, pancreas, and kidney tissues, but not in noticeably decreased concentrations in heart and brain tissues. The presence of both Se and the CLA isomer(s) produced less consistent changes in the capacity of Δ9 desaturase and monounsaturated fatty acid concentrations compared with the effect of the diet with the CLA isomer(s) only.

The influence of dietary conjugated linoleic acid isomers and selenized yeast on the fatty acid profile of the spleen, pancreas and kidneys of rats

“…So, our current results are in agreement with the observations of Alasnier et al (2002) and our previous studies on rats (Czauderna et al, 2003b(Czauderna et al, , 2004aNiedźwiedzka et al, 2006) showing that in the cells of the pancreas, kidneys, brain, liver, muscles and adipose tissues, t10c12 and t10t12CLA isomers are more efficiently driven through β-oxidation than their 9,11 homologues. In contrast, the amount of c9t11 and t10c12 in the spleen of rats fed the diets enriched in the CLA isomer mixture were similar.…”

“…In contrast, the amount of c9t11 and t10c12 in the spleen of rats fed the diets enriched in the CLA isomer mixture were similar. So, the current results are in agreement with our previous observations in which the amounts of these two CLA isomers in the spleen of rats fed diets enriched in the CLA isomer mixture, irrespective of the presence of selenate, was also similar to their amount in dietary CLA isomer mixtures (Niedźwiedzka et al, 2006). The presented results suggest that in rat spleen, the rates of metabolism of dietary c9t11 and t10c12 are similar.…”

“…Our current results as well as our previous studies (Niedźwiedzka et al, 2006) have clearly shown that dietary CLA isomer(s), regardless of the addition of Se-yeast to diets, usually showed a tendency to increase or statistically increased the concentration of linoleic acid (LA) in all examined organs (Tables 3-8). The stimulating influence of the CLA isomer mixture on the accumulation of LA in these organs is stronger if the diet is enriched in a higher concentration of CLA isomers.…”

“…However, it is also possible that the yield of c9t11 accumulation is lower in comparison with t10c12. This second suggestion is fully supported by the results summarized in Table 3 and our previous studies (Niedźwiedzka et al, 2006); indeed, the concentration of c9t11 was lower than the concentration of t10c12 in spleen of rats fed diets enriched in the same concentration of the individual isomer (i.e. c9t11 and t10c12, respectively; see the groups c9t11 and t10c12).…”

Influence of selenate and linseed oil on fatty-acid and amino-acid profiles in the liver, muscles, fat tissues and blood plasma of sheep

“…Indeed, many epidemiological and experimental studies (Niedźwiedzka et al, 2006;Murphy et al, 2007) documented that A-SFA (C12:0, C14:0 and C16:0) and T-SFA (C14:0, C16:0 and C18:0) possessed atherogenic and thrombogenic properties, while α-linolenic acid and its products of elongation and desaturation (i.e. long-chain n-3PUFA) improved anti-infl ammatory status, immune response, as well as benefi ted the cardiovascular system by decreasing platelet aggregation, serum triglycerides and cholesterol (Raes et al, 2004;Flachowsky et al, 2006).…”