The influence of direct laser metal sintering implants on the early stages of osseointegration in diabetic mini-pigs

Abstract

Background High failure rates of oral implants have been reported in diabetic patients due to the disruption of osseointegration. The aim of this study was to investigate whether direct laser metal sintering (DLMS) could improve osseointegration in diabetic animal models. Methods Surface characterizations were carried out on two types of implants. Cell morphology and the osteogenic-related gene expression of MG63 cells were observed under conditions of DLMS and microarc oxidation (MAO). A diabetes model in mini-pigs was established by intravenous injection of streptozotocin (150 mg/kg), and a total of 36 implants were inserted into the mandibular region. Micro-computed tomography (micro-CT) and histologic evaluations were performed 3 and 6 months after implantation. Results The Ra (the average of the absolute height of all points) of MAO surface was 2.3±0.3 µm while the DLMS surface showed the Ra of 27.4±1.1 µm. The cells on DLMS implants spread out more podia than those on MAO implants through cell morphology analysis. Osteogenic-related gene expression was also dramatically increased in the DLMS group. Obvious improvement was observed in the micro-CT and Van Gieson staining analyses of DLMS implants compared with MAO at 3 months, although this difference disappeared by 6 months. DLMS implants showed a higher bone–implant contact percentage (33.2%±11.2%) at 3 months compared with MAO group (18.9%±7.3%) while similar results were showed at 6 months between DLMS group (42.8%±10.1%) and MAO group (38.3%±10.8%). Conclusion The three-dimensional environment of implant surfaces with highly porous and fully interconnected channel and pore architectures can improve cell spreading and accelerate the progress of osseointegration in diabetic mini-pigs.

Restoration of electrical microenvironment enhances bone regeneration under diabetic conditions by modulating macrophage polarization

“…These results thus indicate that the polarized BTO/P(VDF-TrFE) nanocomposite membrane is a promising immunomodulatory biomaterial that can regulate local inflammation to provide a favorable osteo-immunomodulatory microenvironment, thereby accelerating bone regeneration under DM condition. Surface properties of implanted biomaterials have long been considered as key parameter that could guide macrophage polarization and improve bone healing efficiency under diabetic conditions [ [29] , [30] , [31] ]. Therefore, our results identified electrical properties as a new crucial parameter of biomaterials that can modulate macrophage polarization and bone regeneration.…”

Current trends of the choice and processing of materials for dental implantation

“…Модификация поверхности имплантатов важна для улучшения их остеоинтеграции [3,12,41,43,45,48,50,51].…”

“…Вместе с тем в литературе приводятся данные, согласно которым остеоинтеграция имплантатов практически не зависит от характера поверхности [1,19,25,45,46].…”

“…Через 3 мес после операции контакт имплантата с костной тканью составлял соответственно 33,2±11,2 и 18,9±7,3% для металла с увеличенной пористостью поверхности и без нее. Однако к 6 мес произошло выравнивание всех показателей; например, площадь контакта стала составлять соответственно 42,8±10,1 и 38,3±10,8% [45].…”

Evaluation of Effect of Low-Dose Methotrexate on Osseointegration of Implants: A Biomechanical Study on Dogs

“…The second stage is the attachment of osteoblasts to the surface of dental implant leading to a series of complex bone remodeling processes including osseointegration and bone resorption. The last stage is the formation of a close relation between bone and implant connected by collagenous filaments providing the long-term function of dental implant [ 3 ].…”