The influence of early cleavage on embryo developmental potential and IVF/ICSI outcome

Fu, Jing; Wang, Xianjing; Wang, Yongwei; Sun, Jian; Gemzell‐Danielsson, Kristina; Sun, Xiaoxi

doi:10.1007/s10815-009-9342-6

Cited by 26 publications

(14 citation statements)

References 18 publications

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“…In contrast, higher embryo production was obtained in autumn and spring. These results support the cleavage rate as a good predictor of the in vitro blastocyst development (Cetinkaya et al., ; Fu et al., ). Accordingly, the results show a significant decrease in cleavage rates and blastocyst production during winter, corroborating our hypothesis.…”

Section: Discussionsupporting

confidence: 81%

Decrease in bovine in vitro embryo production efficiency during winter season in a warm-summer Mediterranean climate

et al. 2016

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Retrospective analysis of monthly embryo production from December 2011 to May 2015 and its correlation with meteorological data in our geographic zone was made. We had observed that in certain time of the year, in vitro blastocyst production decreases. Accordingly, was examined the association between blastocyst production and climatological parameters. Cleavage rates correlate positively with blastocyst rates (p < .05). Significant differences in cleavage rates between autumn and summer (79.8%; 71.5%), and between winter and autumn (71.8%; 79.8%), were found. Blastocyst production had lower efficiency in June (9 ± 12%) and July (4.9 ± 5.7%), which coincides with winter season. In contrast, higher embryo production was obtained in February (22.2 ± 9.7%), March (22.9 ± 14%) and September (25.2 ± 6.6%), which coincides with autumn and spring season. Similarly, embryo production correlates with meteorological parameters: blastocyst production positively correlates with sunshine hours, maximum temperature and average temperature. Similarly, blastocyst production inversely correlates with total precipitation and days >1 mm precipitation (p < .05). There is a significant decrease in bovine in vitro embryo production efficiency during winter season in our warm-summer Mediterranean climate zone. It remains to be investigated the direct effect of environmental factors on oocyte quality and its impact on in vitro production efficiency.

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Section: Discussionsupporting

confidence: 81%

Decrease in bovine in vitro embryo production efficiency during winter season in a warm-summer Mediterranean climate

et al. 2016

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“…Following fertilization, the superiority of early cleaving embryos over that of late cleaving ones has been reported across many species (Gonzales et al 1995;Totey et al 1996;Van Soom et al 1997;Lonergan et al 1999;Dang-Nguyen et al 2010;Orozco-Lucero et al 2014) establishing that the early cleaving IVF embryos are more likely to develop to the blastocyst earlier and that they are likely to have better quality compared to late cleaving IVF embryos. In humans, extensive studies have been conducted to correlate the time of cleavage with the developmental competence of IVF embryos (Fenwick et al 2002;Salumets et al 2003;Fu et al 2009;Lee et al 2012). Also, transfer of early cleaving bovine IVF embryos has been found to result in pregnancy more often than from late cleaving embryos .…”

Section: Discussionmentioning

confidence: 99%

Early Cleavage of Handmade Cloned Buffalo (Bubalus bubalis) Embryos is an Indicator of Their Developmental Competence and Quality

Kaith

Saini

Raja

et al. 2015

Reprod Domestic Animals

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Following IVF, embryos which cleave early have been shown to have higher developmental competence and quality than those that cleave relatively later across many species. We investigated the effect of time of cleavage on the developmental competence, quality, epigenetic status and gene expression in buffalo embryos produced by handmade cloning (HMC). Following classification of embryos as early cleaving (EC) or late cleaving (LC) based on whether they had cleaved or not at 24 h post in vitro culture, 54% (164/303) were found to be EC and the rest to be LC. The blastocyst rate (58.1 ± 3.4 vs 36.9 ± 1.6%, p < 0.01) and the total cell number (285.5 ± 41.9 vs 141.4 ± 36.1, p < 0.05) were higher, whereas the apoptotic index (3.6 ± 0.6 vs 12.2 ± 1.7, p < 0.01) and the global level of H3K9ac and H3K27me3 were lower (p < 0.05) in the blastocysts produced from EC than in those produced from LC embryos. The relative transcript level of CASPASE3, CASPASE7, DNMT1, DNMT3a and CDX2 was higher (p < 0.05) and that of SOX2 was lower (p < 0.05) in blastocysts produced from LC than in those produced from EC embryos, whereas the expression level of CASPASE6, P53, P21, HDAC1, OCT4 and NANOG was not significantly different between the two groups. These results show that (i) following HMC, blastocysts produced from embryos that cleave early differ from those produced from late cleaving embryos in terms of epigenetic status and expression level of many important apoptosis-, pluripotency-, trophectoderm- and epigenetics-related genes, and (ii) EC embryos are superior to LC embryos in view of their higher developmental competence and quality.

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“…Better oocyte and embryo quality led to high pregnancy rates [33]. However, a follicular fluid biomarker that directly indicates embryo quality has not yet been discovered.…”

Section: Discussionmentioning

confidence: 99%

Do follicular fluid gelatinase levels affect fertilization rates and oocyte quality?

Bilen

Tola

Oral

et al. 2014

Arch Gynecol Obstet

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The influence of early cleavage on embryo developmental potential and IVF/ICSI outcome

Abstract: Early cleavage is a strong indicator of embryo quality, and may be used as an additional criterion in the selection of embryos for transfer to increase pregnancy rate and reduce multiple pregnancy rate.

Cited by 26 publications

References 18 publications

Decrease in bovine in vitro embryo production efficiency during winter season in a warm-summer Mediterranean climate

Decrease in bovine in vitro embryo production efficiency during winter season in a warm-summer Mediterranean climate

Early Cleavage of Handmade Cloned Buffalo (Bubalus bubalis) Embryos is an Indicator of Their Developmental Competence and Quality

Do follicular fluid gelatinase levels affect fertilization rates and oocyte quality?

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