The influence of efflux pump, outer membrane permeability and β-lactamase production on the resistance profile of multi, extensively and pandrug resistant Klebsiella pneumoniae

Hussein, Rawaa A.; AL-Kubaisy, Shaymaa H.; Al-Ouqaili, Mushtak T.S.

doi:10.1016/j.jiph.2024.102544

Cited by 2 publications

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

An investigation of carbapenemase-encoding genes inBurkholderia cepaciaandAeromonas sobrianosocomial infections among Iraqi patients

Al-Ouqaili,

Hussein,

Kanaan

et al. 2024

Preprint

View full text Add to dashboard Cite

Burkholderia cepacia and Aeromonas sobria are difficult to eradicate due to their innate resistance to a variety of medications, and cause various diseases. The aim of this study was to investigate the occurrence of carbapenemase genes and patterns of antibiotic resistance in isolates of B. cepacia and A. sobria. A cross-sectional study was conducted in the Ramadi Teaching Hospitals in the Al-Anbar Governorate in 2024. Various study samples, were used to collect the studied bacteria. The antibiotic resistance was detected by the VITEK®2 System. The presence of carbapenemase genes was confirmed via PCR technique. In this investigation, seventy-five (75) isolates of A. sobria and B. cepacia were assessed. Of these, A. sobria made up 16.6% (n = 20) while B. cepacia accounted for 45.8% (n = 55). The study isolates showed highest antimicrobial resistance to piperacillin, cefepime, ceftriaxone (100%), ceftazidime (97.3%), and lowest antimicrobial resistance to imipenem (36%). The result showed 55/57 recA gene positive for differentiated B. cepacia complex from other Burkholderia spp. The overall prevalence of carbapenemase genes was 92.8%% (52/56) with blaKPC accounting for 80.8% (42/52) and blaGES for 19.2% (10/52) of the total. The 42 B. cepacia isolates that tested positive for carbapenem resistance were constituted of 38 blaKPC (n = 38) and two blaGES (n = 2); in contrast, four blaKPC (n = 4) and eight blaGES (n = 8) were present in the A. sobria isolates that tested positive for carbapenems resistance. None of isolates studied tested positive for the blaIMP gene. The recent study concluded that recA gene identification was more sensitive and specific technique for detection B. cepacia complex isolates. There was a notable predominance of blaKPC and blaGES carbapenemase producers among the isolates under investigation. The blaIMP gene was not found in any of the research isolates.

show abstract