The influence of electrically conductive and non-conductive nanocomposite scaffolds on the maturation and excitability of engineered cardiac tissues

Navaei, Ali; Eliato, Kiarash Rahmani; Ros, Robert; Migrino, Raymond Q.; Willis, Brigham C.; Nikkhah, Mehdi

doi:10.1039/c8bm01050a

Cited by 56 publications

(31 citation statements)

References 72 publications

(105 reference statements)

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“…Addition of nanosized PEDOT:PSS gives only modest improvements in measured conductivity, while simultaneously introducing topological features. As was presented by other researchers, conductivity can sometimes emerge subordinate to nanoscale roughness …”

Section: Conductive Scaffolds For Cardiac Tissue Engineeringmentioning

confidence: 63%

“…Resilience to gap‐junction decoupling has been attributed to conductive scaffold‐mediated intercellular communication; however, nonconductive silica nanoparticles embedded in a scaffold can produce strong cardiomyocyte tissues that show synonymous resilience to conductive AuNPs scaffolds . This is a result of topography‐induced protein adhesion, a feature that often correlates with amount of dopant .…”

Section: Discussion and Open Questions For The Use Of Conductive Tissmentioning

confidence: 99%

“…Although these studies present convincing hypotheses, reciprocal experiments show that there are alternative paths to produce similar results. Navaei et al discussed in further detail later, shows that both nonconductive Si nanoparticles (NPs) and conductive gold nanoparticles (AuNPs) doped onto a gelatin methacrylate substrate demonstrate equivalent resiliency in the heptanol synchronicity experiment. This finding implies that the conductivity of the dopant not only is secondary to topographical cues, but could even be irrelevant.…”

Section: Conductive Scaffolds For Cardiac Tissue Engineeringmentioning

confidence: 96%

“…Continuing with an important follow up 2018 study, Navaei et al sought to independently examine the often entangled roles of conductivity, topology, and stiffness in tissue engineering scaffold application. A control 5% GelMA hydrogel was compared to one loaded with nonconductive silica nanoparticle (SNP) and another with conductive AuNR of comparable size, concentration, and capping functionalization; also included was a 20% GelMA to increase stiffness intrinsically.…”

Section: Conductive Scaffolds For Cardiac Tissue Engineeringmentioning

confidence: 99%

“…The extracted beating signals of stimulated cardiac tissues upon 20 min of treatment with heptanol (2 × 10 −3 m ), demonstrating that GelMA–SNP and GelMA–GNR hydrogels accommodated electrical stimuli with higher frequencies (up to 2 Hz) in comparison with GelMA (20%) (up to 1 Hz). Reproduced with permission . Copyright 2018, The Royal Society of Chemistry.…”

Section: Conductive Scaffolds For Cardiac Tissue Engineeringmentioning

confidence: 99%

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Conductive Scaffolds for Cardiac and Neuronal Tissue Engineering: Governing Factors and Mechanisms

Burnstine‐Townley

Eshel

Amdursky

2019

Adv Funct Materials

119

102

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Tissue engineering is a promising therapeutic approach in medicine, targeting the replacement of a diseased tissue with a healthy one grown within an artificial scaffold. Due to the high prevalence of cardiac and brain‐related ailments that involve some necrosis of tissue, cardiac and neuronal tissue engineering are intensely studied fields in regenerative medicine. A growing trend in the use of conductive scaffolds for the growth of these tissues has been witnessed recently. While the results are irrefutable, the mechanism of how an electrically conducting scaffold interacts with an electroactive tissue remains has remained elusive. An up‐to‐date summary of all work done in the field is reported, with a special focus on the specific contribution of the conductive scaffold on the performance of the formed tissue. The cell–scaffold electronic interface is then explored from an electrical engineering perspective. The electronic configuration of the system and the mechanisms and governing factors controlling the ability of a conductive scaffold to support cardiac and neuronal tissues are discussed. Using several simulations, the required conductivity of the scaffold in order for it to be suitable for tissue engineering—which also depends on the nature of the charge carriers—is also discussed.

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Section: Conductive Scaffolds For Cardiac Tissue Engineeringmentioning

confidence: 63%

Section: Discussion and Open Questions For The Use Of Conductive Tissmentioning

confidence: 99%

Section: Conductive Scaffolds For Cardiac Tissue Engineeringmentioning

confidence: 96%

Section: Conductive Scaffolds For Cardiac Tissue Engineeringmentioning

confidence: 99%

Section: Conductive Scaffolds For Cardiac Tissue Engineeringmentioning

confidence: 99%

See 3 more Smart Citations

Conductive Scaffolds for Cardiac and Neuronal Tissue Engineering: Governing Factors and Mechanisms

Burnstine‐Townley

Eshel

Amdursky

2019

Adv Funct Materials

119

102

View full text Add to dashboard Cite

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Conductive and injectable hyaluronic acid/gelatin/gold nanorod hydrogels for enhanced surgical translation and bioprinting

Kiyotake

Thomas

Homburg

et al. 2021

J Biomedical Materials Res

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There is growing evidence indicating the need to combine the rehabilitation and regenerative medicine fields to maximize functional recovery after spinal cord injury (SCI), but there are limited methods to synergistically combine the fields. Conductive biomaterials may enable synergistic combination of biomaterials with electric stimulation (ES), which may enable direct ES of neurons to enhance axon regeneration and reorganization for better functional recovery; however, there are three major challenges in developing conductive biomaterials: (1) low conductivity of conductive composites, (2) many conductive components are cytotoxic, and (3) many conductive biomaterials are pre‐formed scaffolds and are not injectable. Pre‐formed, noninjectable scaffolds may hinder clinical translation in a surgical context for the most common contusion‐type of SCI. Alternatively, an injectable biomaterial, inspired by lessons from bioinks in the bioprinting field, may be more translational for contusion SCIs. Therefore, in the current study, a conductive hydrogel was developed by incorporating high aspect ratio citrate‐gold nanorods (GNRs) into a hyaluronic acid and gelatin hydrogel. To fabricate nontoxic citrate‐GNRs, a robust synthesis for high aspect ratio GNRs was combined with an indirect ligand exchange to exchange a cytotoxic surfactant for nontoxic citrate. For enhanced surgical placement, the hydrogel precursor solution (i.e., before crosslinking) was paste‐like, injectable/bioprintable, and fast‐crosslinking (i.e., 4 min). Finally, the crosslinked hydrogel supported the adhesion/viability of seeded rat neural stem cells in vitro. The current study developed and characterized a GNR conductive hydrogel/bioink that provided a refinable and translational platform for future synergistic combination with ES to improve functional recovery after SCI.

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Recent Development of Conductive Hydrogels for Tissue Engineering: Review and Perspective

Gao

Song

et al. 2022

Macromolecular Bioscience

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In recent years, tissue engineering techniques have been rapidly developed and offer a new therapeutic approach to organ or tissue damage repair. However, most of tissue engineering scaffolds are nonconductive and cannot establish effective electrical coupling with tissue for the electroactive tissues. Electroconductive hydrogels (ECHs) have received increasing attention in tissue engineering owing to their electroconductivity, biocompatibility, and high water content. In vitro, ECHs can not only promote the communication of electrical signals between cells, but also mediate the adhesion, proliferation, migration, and differentiation of different kinds of cells. In vivo, ECHs can transmit the electric signal to electroactive tissues and activate bioelectrical signaling pathways to promote tissue repair. As a result, implanting ECHs into damaged tissues can effectively reconstruct physiological functions related to electrical conduction. In this review, an overview about the classifications and the fabrication methods of ECHs is first presented. And then, the applications of ECHs in tissue engineering, including cardiac, nerve, skin, and skeletal muscle tissue, are highlighted. At last, some rational guidelines for designing ECHs toward clinical applications are provided.

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The influence of electrically conductive and non-conductive nanocomposite scaffolds on the maturation and excitability of engineered cardiac tissues

Abstract: We developed different classes of hydrogels, with conductive and non-conductive nanomaterials, to study cardiac tissue maturation and excitability.

Cited by 56 publications

References 72 publications

Conductive Scaffolds for Cardiac and Neuronal Tissue Engineering: Governing Factors and Mechanisms

Conductive Scaffolds for Cardiac and Neuronal Tissue Engineering: Governing Factors and Mechanisms

Conductive and injectable hyaluronic acid/gelatin/gold nanorod hydrogels for enhanced surgical translation and bioprinting

Recent Development of Conductive Hydrogels for Tissue Engineering: Review and Perspective

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