The influence of electron utilization pathways on photosystem I photochemistry in <i>Synechocystis</i> sp. PCC 6803

Smolinski, Sharon; Lubner, Carolyn E.; Guo, Zhanjun; Artz, Jacob H.; Brown, Katherine A.; Mulder, David W.; King, Paul W.

doi:10.1039/d2ra01295b

Cited by 3 publications

(1 citation statement)

References 49 publications

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“…These results indicate that flavodiiron activity has an influence on photosystem arrangement through its role as an alternative electron transport pathway. Although growth capabilities were not measured for the mutant strains, lowered reducing efficiency in P700 was observed, implying that the imbalance between electron generation and utilization at PSI emerged by taking away the electron sink, thus impairing the overall photosynthetic efficiency( Smolinski et al., 2022 ). This could represent a way to diminish photosystem I action to balance reduced light absorption at photosystem II light harvesting antenna.…”

Section: Cyanobacterial Photosynthesismentioning

confidence: 99%

Advances in light system engineering across the phototrophic spectrum

Dennis,

Posewitz

2024

Front. Plant Sci.

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Current work in photosynthetic engineering is progressing along the lines of cyanobacterial, microalgal, and plant research. These are interconnected through the fundamental mechanisms of photosynthesis and advances in one field can often be leveraged to improve another. It is worthwhile for researchers specializing in one or more of these systems to be aware of the work being done across the entire research space as parallel advances of techniques and experimental approaches can often be applied across the field of photosynthesis research. This review focuses on research published in recent years related to the light reactions of photosynthesis in cyanobacteria, eukaryotic algae, and plants. Highlighted are attempts to improve photosynthetic efficiency, and subsequent biomass production. Also discussed are studies on cross-field heterologous expression, and related work on augmented and novel light capture systems. This is reviewed in the context of translatability in research across diverse photosynthetic organisms.

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Section: Cyanobacterial Photosynthesismentioning

confidence: 99%

Advances in light system engineering across the phototrophic spectrum

Dennis,

Posewitz

2024

Front. Plant Sci.

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Cyanobacteria as whole-cell factories: current status and future prospectives

Toepel

Karande

Klähn

et al. 2023

Current Opinion in Biotechnology

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Alteration of phycobilisome excitation energy transfer properties in response to attenuations in peripheral electron flow

Guo,

Smolinski,

King

et al. 2024

Front. Plant Physiol.

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In Synechocystis sp. PCC 6803 (S. 6803), two types of phycobilisome (PBS) complexes, CpcG-PBS and CpcL-PBS, function to harvest light energy for photosynthetic reaction centers (RCs), photosystem I (PSI) and photosystem II (PSII). The compositional differences between these two forms of PBS and their specificity for RCs have led to suggestions that they may differ in function. To address this question, we examined how PBS-RC interactions, and the transfer of excitation energy from PBS to RCs, might be adjusted under conditions where electron demand and photon availability are modulated. The CpcG-PBS, CpcL-PBS, and RC complexes were isolated from a S. 6803 strain defective in expression of flavodiiron 1 (oxygen reduction reaction 1, ORR1) grown under varied light regimes. The energy transfer preference from CpcL-PBS to either PSI or PSII was investigated by in vitro crosslinking and 77 K fluorescence emission spectroscopy to assess energy transfer efficiency under photoexcitation. While the results demonstrate that the transfer of excitation energy from CpcL-PBS favors PSI over PSII in WT strains as previously shown, the preference of CpcL-PBS switches from PSI to PSII in ORR1 strains. Surprisingly, this change in preference was reproduced when ORR1 CpcL-PBS was crosslinked with WT RCs, or when WT CpcL-PBS was cross-crosslinked with ORR1 RCs, indicating there are physical modifications to both PBS and RCs that mediate the preference switch. In contrast, the analysis with ORR1 CpcG-PBS shows similar preferences to WT. Additionally, PBS populations in ORR1 shifted to a greater proportion of CpcL-PBS relative to CpcG-PBS. These results demonstrate that under conditions where electron utilization changes, there is a tuning of the excitation energy allocation from CpcL-PBS to RCs to manage the energy distribution for photosynthesis under dynamic flux conditions.

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The influence of electron utilization pathways on photosystem I photochemistry in Synechocystis sp. PCC 6803

Abstract: Alteration of electron utilization pathways affects PSI reaction center structure and photochemical properties.

Cited by 3 publications

References 49 publications

Advances in light system engineering across the phototrophic spectrum

Advances in light system engineering across the phototrophic spectrum

Cyanobacteria as whole-cell factories: current status and future prospectives

Alteration of phycobilisome excitation energy transfer properties in response to attenuations in peripheral electron flow

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