The Influence of Enamel Matrix Derivative Associated With Insulin‐Like Growth Factor‐I on Periodontal Ligament Fibroblasts

Palioto, Daniela Bazan; Coletta, Ricardo D.; Graner, Edgard; Joly, Júlio César; Lima, Antônio Fernando Martorelli de

doi:10.1902/jop.2004.75.4.498

Cited by 45 publications

(52 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several studies have demonstrated that IGF-1 can stimulate the proliferation of PDL cells, promote the wound healing of PDL, maintain periodontal homeostasis, and even elongate the molar roots (Fujiwara et al 2005;Palioto et al 2004;Gestrelius et al 1997;Kheralla et al 2010;Lossdorfer et al 2009;Caton et al 2005;Han et al 2007;Giannobile 1996;GraneroMolto et al 2011). Thus, it is reasonable that IGF-1 may have some influence on the differentiation of PDLSCs.…”

Section: Discussionmentioning

confidence: 99%

Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways

Fan

et al. 2012

Histochem Cell Biol

124

View full text Add to dashboard Cite

Insulin-like growth factor 1 (IGF-1) is a potent mitogenic protein which can enhance the osteogenic differentiation of periodontal ligament (PDL) fibroblasts. However, it remains unclear whether IGF-1 can stimulate the osteogenic differentiation and osteogenesis of human periodontal ligament stem cells (PDLSCs). In this study, STRO-1(+) PDLSCs were isolated from human PDL tissues, treated with IGF-1, and their osteogenic capacity was investigated in vitro and in vivo. Dimethyl-thiazol-diphenyl tetrazolium bromide assay and flow cytometry results demonstrated that 10-200 ng/mL IGF-1 can stimulate the proliferation ability of PDLSCs and 100 ng/mL is the optimal concentration. Exogenous IGF-1 can modify the ultrastructure, enhance the alkaline phosphatase activity, the mineralization ability of PDLSCs, and increase the expression of osteogenic markers (runt-related transcription factor 2, osterix, and osteocalcin) at mRNA and protein levels. In vivo transplantation illustrated that IGF-1 treated implants generated more mineralized tissues, and presented stronger expression of RUNX2, OSX, and OCN than control group. Moreover, the expression of phosphor-ERK and phosphor-JNK in these stem cells was upregulated by IGF-1, indicating that MAPK signaling pathway was activated during the osteogenic differentiation of PDLSCs mediated by IGF-1. Together, the results showed that IGF-1 can promote the osteogenic differentiation and osteogenesis of STRO-1(+) PDLSCs via ERK and JNK MAPK pathway, suggesting that IGF-1 is a potent agent for stem cell-based periodontal tissue regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways

Fan

et al. 2012

Histochem Cell Biol

124

View full text Add to dashboard Cite

show abstract

“…This strategy that exploits the regenerative capacity of stem cells grown in a three-dimensional scaffold and subsequently implanted into the defect, in the presence of molecule modulators, may help overcoming several limitations of current regeneration modalities 1 . In an in vitro study, the application of growth factors found in PRP (TGF-β1 and IGF-I) in cultures of periodontal ligament cells, increased their mitotic activity and their capacity for adhesion on the root fragments surface 20,23 . The use of platelet growth factors associated with cell therapy could be an alternative favoring the process of periodontal regeneration.…”

Section: Discussionmentioning

confidence: 99%

Platelet-rich plasma plus bioactive glass in the treatment of intra-bony defects: a study in dogs

et al. 2011

View full text Add to dashboard Cite

ObjectiveThis study was designed to evaluate, histomorphometrically, the association of platelet-rich plasma (PRP) and bioactive glass (BG) in the treatment of periodontal intrabony defects.Material and MethodsNine mongrel dogs were included in the study. Three-wall intrabony defects were surgically created at the mesial and distal aspect of first mandibular molar and exposed to plaque accumulation for 1 month. The defects were randomly assigned to the groups: control, BG, PRP, PRP+BG. Dogs were sacrificed 90 days after the surgeries. The histometric parameters evaluated were: length of sulcular and junctional epithelium, connective tissue adaptation, new cementum, new bone, defect extension and area of new bone filling the defect.ResultsA superior area of new bone was observed in PRP+BG and BG (13.80±2.32 mm2 and 15.63±2.64 mm2, respectively) when compared to the other groups (8.19±1.46 mm2 and 8.81±1.47 mm2 for control and PRP, respectively). No statistically significant differences were observed in the remaining parameters.ConclusionWithin the limits of this study, it may be concluded that PRP failed to provide statistically significant improvements in the histometric parameters.

show abstract

“…In summary, it appears that EMD stimulates growth in numerous mesenchymal cell types and is thought to function through enhancing cell attachment [31][32][33][34] and possessing growth factor-like activity. 26,27,30,31,[35][36][37][38][39][40][41][42][43][44][45][46][47][48] The findings of these previous studies led us to hypothesize that EMD could have a therapeutic use in ligament tissue engineering. Our hypothesis is that EMD may, through the promotion of good, early tissue induction, protect an in situ tissue-engineered ligament prosthesis from abrasion against bone tunnels and (consequent) resultant rupture.…”

Section: Introduction I Njury Of the Anterior Cruciate Ligament (Acl) Ismentioning

confidence: 99%

The Potential Use of Enamel Matrix Derivative for In Situ Anterior Cruciate Ligament Tissue Engineering: A Translational In Vitro Investigation

et al. 2007

View full text Add to dashboard Cite

Polyester scaffolds have been used as an alternative to autogenous tissues for the reconstruction of the anterior cruciate ligament (ACL). They are biocompatible and encourage tissue infiltration, leading to neoligament formation. However, rupture can occur, caused by abrasion of the scaffold against the bone tunnels through which it is implanted. Good early tissue induction is therefore considered essential to protect the scaffold from this abrasion. Enamel matrix derivative (EMD) is used clinically in the treatment of periodontal disease. It is a complex mix of proteins with growth factor-like activity, which enhances periodontal ligament fibroblast attachment, proliferation, and differentiation, leading to the regeneration of periodontal bone and ligament tissues. We hypothesized that EMD might, in a similar manner, enhance tissue induction around scaffolds used in ACL reconstruction. This preliminary investigation adopted a translational approach, modelling in vitro 3 possible clinical modes of EMD administration, to ascertain the suitability of each protocol for application in an animal model or clinically. Preliminary investigations in monolayer culture indicated that EMD had a significant dose-dependent stimulatory effect (p < 0.05, n = 6) on the proliferation of bovine primary synovial cells. However, pre-treating culture plates with EMD significantly inhibited cell attachment (p < 0.01, n = 6). EMD's effects on synovial cells, seeded onto ligament scaffolds, were then investigated in several in vitro experiments modelling 3 possible modes for clinical EMD administration (pre-, intra-, and post-operative). In the pre-operative model, EMD was adsorbed onto scaffolds before the addition of cells. In the intra-operative model, EMD and cells were added simultaneously to scaffolds in the culture medium. In the post-operative model, cells were pre-seeded onto scaffolds before EMD was administered. EMD significantly inhibited cell adhesion in the pre-operative model (p < 0.05, n = 6) and had no significant benefit in the intra-operative model. In the post-operative model, the addition of EMD to previously cell-seeded scaffolds significantly increased their total deoxyribonucleic acid content (p < 0.01, n = 5). EMD's stimulative effect on cell proliferation in vitro suggests that it may accelerate scaffold colonization by cells (and in turn tissue induction) in situ. However, its inhibitory effect on synovial cell attachment in vitro implies that it may only be suited to post-operative administration.

show abstract

The Influence of Enamel Matrix Derivative Associated With Insulin‐Like Growth Factor‐I on Periodontal Ligament Fibroblasts

Abstract: Our results showed that EMD, IGF-I, and the combination of both factors stimulated PDL fibroblast proliferation, whereas these factors did not affect adhesion, migration, or expression of type I collagen of these cells.

Cited by 45 publications

References 37 publications

Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways

Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways

Platelet-rich plasma plus bioactive glass in the treatment of intra-bony defects: a study in dogs

The Potential Use of Enamel Matrix Derivative for In Situ Anterior Cruciate Ligament Tissue Engineering: A Translational In Vitro Investigation

Contact Info

Product

Resources

About