Three trials of 6-month duration each were conducted with 24-week old Leghorn pullets in five environmental chambers. In each trial two birds were placed in each of 54 cages in each chamber. All birds were exposed to a 14-hour light regime and to one of four relative humidity levels (50, 60, 70, 80%) and one of three dry bulb temperatures (13°, 21.5°, 29.5° C). Isonitrogenous diets of varying energy levels [3080, 2648, 2220 kilocalories of metabolizable energy per kilogram (kcal. M.E./kg.)] were fed ad libitum in each chamber. Parameters in all trials included body weight gains, hen-day production, egg weight, specific gravity, Haugh units, feed intake and excretal output.Statistical analysis indicated that the best overall performance was obtained with a dry bulb temperature of either 13° or 21.5° C. and with a dietary energy level of 2648 kcal. M.E./kg. The relative humidity exerted no significant effects on any of the parameters except excretal output at 29.5° C. At this temperature, a significant linear relationship was observed between relative humidity and excretal output as well as excretal moisture. The most striking observation was the inverse relationship of dietary energy to feed intake and excretal output, irrespective of temperature and relative humidity, and also the inverse relationship of temperature to excretal output, irrespective of relative humidity and dietary energy.