The influence of epidermal growth factor on cat corneal endothelial wound healing

Rich, Larry F.; Hatfield, Joanna; Louiselle, I

doi:10.3109/02713689109013878

Cited by 15 publications

(2 citation statements)

References 12 publications

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“…HA has been used in other systems to deliver growth factors, including the eye. Rich et al 39 showed improved wound healing in cat eyes treated with EGF solubilized in HA. Similarly, Raphael et al .4°i n 1993 showed that, after an endothelial cell injury, a single intraocular injection of EGF formulated in sodium hyaluronate significantly enhanced multiple parameters that are closely related to improved endothelial cell regeneration .…”

Section: Wound Repair and Regeneration January-march 7997mentioning

confidence: 98%

Hyaluronan: a potential carrier for growth factors for the healing of ligamentous tissues

Berry¹,

Green

Amiel³

1997

Wound Repair Regeneration

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The anterior cruciate ligament has little capacity to heal after injury, in contrast to the medial collateral ligament. Cell migration and cell proliferation are essential steps in the wound healing of connective tissue, and we have developed an in vitro assay to study cell outgrowth (i.e., both cell migration and cell proliferation) from tissue explants. Using this assay, we have previously shown a synergistic effect of four growth factors on the early stages of cell outgrowth (i.e., at days 3 and 6) from these explants. Hyaluronan, a linear polysaccharide with a molecular weight of 3 x 10(6) daltons, has been shown to enhance the anterior cruciate ligament healing capacity in vivo by over 50%. The purpose of this study was to assess in vitro whether hyaluronan and the combination of growth factors (transforming growth factor-beta1 25 ng/ml, platelet-derived growth factor-BB 100 ng/ml, basic fibroblast growth factor 100 ng/ml, and insulin 25 microg/ml) would enhance cell outgrowth from explants at 3 and 6 days. At 3 days the mean cell outgrowth from the explants treated with hyaluronan and the combination of growth factors exceeded the growth in the explants treated with growth factors alone for the anterior cruciate ligament (mean cell count +/- standard error: 19 +/- 4 and 2 +/- 1 cells, respectively) and for the medial collateral ligament (297 +/- 40 and 87 +/- 18 cells, respectively). At 6 days, the mean cell outgrowth in the presence of growth factors was enhanced sixfold by hyaluronan for the anterior cruciate ligament (160 +/- 27 compared with 26 +/- 15 cells) and fourfold for the medial collateral ligament (1363 +/- 219 compared with 330 +/- 74 cells). As previously shown, little cell outgrowth occurred from the anterior cruciate or medial collateral ligament explants when no growth factors were added to the modified Eagle's media at 3 and 6 days (<20 cells). For the medial collateral ligament only, hyaluronan had a small stimulatory effect on cell outgrowth in minimal essential media in the absence of growth factors. At 3 days the stimulation by hyaluronan was fourfold (31 +/- 8 cells compared with 7 +/- 4 cells), and at 6 days the stimulation by hyaluronan was nearly fourfold (73 +/- 17 compared with 20 +/- 12 cells). These results suggest that hyaluronan could be used together with growth factors in efforts to enhance the healing of ligamentous tissue injuries.

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Section: Wound Repair and Regeneration January-march 7997mentioning

confidence: 98%

Hyaluronan: a potential carrier for growth factors for the healing of ligamentous tissues

Berry¹,

Green

Amiel³

1997

Wound Repair Regeneration

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“…These proteins constitute less than 0.4% of the protein in MGF-I1 but retain greater than 90% of MGF-II's capacity to stimulate DNA synthesis in cultured AKR-2B cells and rabbit stromal fibroblasts(22).Together, the current cell culture data, the 1982 report, and the results of our more recent fractionation studies indicate that only a limited amount of the R-MGF prepa-ration could be EGF: on the order of 10 to 500 n g / d at the 2.5 to 100 pg/ml MGF dosages used in this study.Other studies in this laboratory have shown that final aqueous dosages of EGF ranging from 3.3 to 167 p g / d , based on an anterior chamber volume of 600 pl in the cat, did not stimulate endothelial wound healing when administered in PBS. Even when EGF was administered in the viscoelastic agent hyaluronic acid, dosages of pure EGF ranging from 3.3 to 50 pg/ml were required to accelerate wound closure(23).The possibility exists that low levels of EGF may be interacting with other components of R-MGF to stimulate the wound healing seen in the present study. Alternatively, the kallikreins identified in MGF-I and -11 (21) along with the kallikrein activities identified in MGF-I11 and -1V…”

mentioning

confidence: 73%

Stimulation of corneal endothelial wound healing in cats by mesodermal growth factor

Louiselle

et al. 1990

Current Eye Research

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Several growth factors have been evaluated for their effects on corneal wound healing but few studies have yet demonstrated an acceleration of endothelial repair in vivo. Mesodermal Growth Factor (MGF) was tested in vivo by making standardized freeze wounds in cat corneas and immediately injecting one of four concentrations of MGF in sterile phosphate buffered saline (PBS), or PBS alone, into the anterior chamber. Seven days later, the animals were sacrificed and the corneas excised and stained. Descemet's membrane and endothelium were dissected and mounted onto glass slides. The wound areas were photographed, measured and compared statistically. Those cats receiving the three lowest doses of MGF had significantly smaller wounds than controls (p less than 0.05).

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Specific simple sugars promote chemotaxis and chemokinesis of corneal endothelial cells

Vogel

Blake

Whikehart

et al. 1993

Journal Cellular Physiology

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Bovine corneal endothelial cells showed a strong migratory response to specific simple sugars (D-glucose and sucrose, but not L-glucose, sorbitol, lactose, or D-galactose) at concentrations above 10 mM. Checkerboard analysis of the migratory responses in modified Boyden chambers indicated both chemotactic and chemokinetic effects. Serum starvation of the cultures increased the chemotaxis towards D-glucose and 2-deoxy-D-glucose, but not towards sucrose. Migration to sucrose and glucose was inhibited by chelation of extracellular calcium or by inhibition of Na+, K+ ATPase with ouabain. To date, this migratory response has been found only in corneal endothelial cells. Neither human melanoma cells, human breast carcinoma cells, bovine aortic endothelial cells, nor bovine microvascular endothelial cells migrated towards simple sugars, although all cell types migrated toward fibronectin in chemotaxis assays. After 16-19 passages in culture, bovine corneal endothelial cells retained their ability to migrate towards fibronectin, but lost their ability to migrate towards sugars. This loss of migratory response was accompanied by a sevenfold decrease in Na+, K+ ATPase activity. Although loss of Na+, K+ ATPase activity accompanied the loss of migratory response, pretreatment of cell cultures with 25 mM glucose did not stimulate, but rather lowered Na+, K+ ATPase activity in low or high passage cultures.

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The influence of epidermal growth factor on cat corneal endothelial wound healing

Cited by 15 publications

References 12 publications

Hyaluronan: a potential carrier for growth factors for the healing of ligamentous tissues

Hyaluronan: a potential carrier for growth factors for the healing of ligamentous tissues

Stimulation of corneal endothelial wound healing in cats by mesodermal growth factor

Specific simple sugars promote chemotaxis and chemokinesis of corneal endothelial cells

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